Effects of floral symmetry and orientation on the consistency of pollinator entry angle

Since the publication of Sprengel’s (1793) observations, it has been considered that flowers with zygomorphic (or bilaterally symmetrical) corollas evolved to restrict the movement of pollinators into the flower by limiting the pollinator’s direction of approach. However, little empirical support has been accumulated so far. Our aim was to build on previous research that showed zygomorphy reduces variance in pollinator entry angle, aiming to observe whether floral symmetry or orientation had an impact on pollinator entry angle in a laboratory experiment using bumble bees, Bombus ignitus. Using nine different combinations of artificial flowers created from three symmetry types (radial, bilateral and disymmetrical) and three orientation types (upward, horizontal, and downward), we tested the effects of these two floral aspects on the consistency of bee entry angle. Our results show that horizontal orientation significantly reduced the variance in entry angle, while symmetry had little effect. We also found either little or no significant interactions between angle and symmetry in their effect on entry angle. Thus, our results suggest that horizontal orientation forces the bees to orient themselves relative to gravity rather than the corolla and stabilizes their flower entry. This stabilizing effect may have been mistaken for the effect of zygomorphic corolla as it is presented horizontally in most species. Consequently, we suggest that the evolution of horizontal orientation preceded that of zygomorphy as indicated by some authors, and that the reason behind the evolution of zygomorphy should be revisited. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00114-023-01845-w.