Cargando…
Implications of Peptidyl Arginine Deiminase 4 gene transcription and polymorphisms in susceptibility to rheumatoid arthritis in an Iranian population
BACKGROUND: Peptidyl arginine deiminase 4 (PADI4) has been implicated in Rheumatoid arthritis (RA) pathogenesis. Here we aimed to evaluate the association of PADI4 gene rs11203367 and rs1748033 single nucleotide polymorphisms (SNPs) with RA proneness. METHODS: The mRNA expression of PADI4 was determ...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10186752/ https://www.ncbi.nlm.nih.gov/pubmed/37193992 http://dx.doi.org/10.1186/s12920-023-01532-9 |
_version_ | 1785042622680662016 |
---|---|
author | Bagheri-Hosseinabadi, Zahra Mirzaei, Mohammad Reza Esmaeili, Ozrasadat Asadi, Fatemeh Ahmadinia, Hassan Shamsoddini, Banafshe Abbasifard, Mitra |
author_facet | Bagheri-Hosseinabadi, Zahra Mirzaei, Mohammad Reza Esmaeili, Ozrasadat Asadi, Fatemeh Ahmadinia, Hassan Shamsoddini, Banafshe Abbasifard, Mitra |
author_sort | Bagheri-Hosseinabadi, Zahra |
collection | PubMed |
description | BACKGROUND: Peptidyl arginine deiminase 4 (PADI4) has been implicated in Rheumatoid arthritis (RA) pathogenesis. Here we aimed to evaluate the association of PADI4 gene rs11203367 and rs1748033 single nucleotide polymorphisms (SNPs) with RA proneness. METHODS: The mRNA expression of PADI4 was determined in the whole blood samples. The genotyping of PADI4 polymorphisms was conducted using allelic discrimination TaqMan genotyping Real-time PCR. RESULTS: The alleles and genotypes of rs11203367 polymorphism were not associated with susceptibility to RA risk. The T allele (OR = 1.58, 95%CI: 1.21–2.04, P = 0.0005), TT genotype (OR = 2.79, 95%CI: 1.53–5.06, P = 0.0007), TC genotype (OR = 1.52, 95%CI: 1.04–2.23, P = 0.0291), dominant (OR = 1.72, 95%CI: 1.19–2.47, P = 0.0034) and recessive (OR = 2.19, 95%CI: 1.25–3.82, P = 0.0057) models of rs1748033 SNP were associated with higher risk of RA. There was a significant upregulation of PADI4 mRNA in the RA patients compared to controls. mRNA expression of PADI4 had significantly positive correlation with anti-CCP level (r = 0.37, P = 0.041), RF level (r = 0.39, P = 0.037), and CRP level (r = 0.39, P = 0.024). CONCLUSION: PADI4 gene rs1748033 SNP was associated with increased RA risk. This polymorphism might affect the RA pathogenesis regardless of impressing the levels of PADI-4 in serum. |
format | Online Article Text |
id | pubmed-10186752 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-101867522023-05-17 Implications of Peptidyl Arginine Deiminase 4 gene transcription and polymorphisms in susceptibility to rheumatoid arthritis in an Iranian population Bagheri-Hosseinabadi, Zahra Mirzaei, Mohammad Reza Esmaeili, Ozrasadat Asadi, Fatemeh Ahmadinia, Hassan Shamsoddini, Banafshe Abbasifard, Mitra BMC Med Genomics Research BACKGROUND: Peptidyl arginine deiminase 4 (PADI4) has been implicated in Rheumatoid arthritis (RA) pathogenesis. Here we aimed to evaluate the association of PADI4 gene rs11203367 and rs1748033 single nucleotide polymorphisms (SNPs) with RA proneness. METHODS: The mRNA expression of PADI4 was determined in the whole blood samples. The genotyping of PADI4 polymorphisms was conducted using allelic discrimination TaqMan genotyping Real-time PCR. RESULTS: The alleles and genotypes of rs11203367 polymorphism were not associated with susceptibility to RA risk. The T allele (OR = 1.58, 95%CI: 1.21–2.04, P = 0.0005), TT genotype (OR = 2.79, 95%CI: 1.53–5.06, P = 0.0007), TC genotype (OR = 1.52, 95%CI: 1.04–2.23, P = 0.0291), dominant (OR = 1.72, 95%CI: 1.19–2.47, P = 0.0034) and recessive (OR = 2.19, 95%CI: 1.25–3.82, P = 0.0057) models of rs1748033 SNP were associated with higher risk of RA. There was a significant upregulation of PADI4 mRNA in the RA patients compared to controls. mRNA expression of PADI4 had significantly positive correlation with anti-CCP level (r = 0.37, P = 0.041), RF level (r = 0.39, P = 0.037), and CRP level (r = 0.39, P = 0.024). CONCLUSION: PADI4 gene rs1748033 SNP was associated with increased RA risk. This polymorphism might affect the RA pathogenesis regardless of impressing the levels of PADI-4 in serum. BioMed Central 2023-05-16 /pmc/articles/PMC10186752/ /pubmed/37193992 http://dx.doi.org/10.1186/s12920-023-01532-9 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Bagheri-Hosseinabadi, Zahra Mirzaei, Mohammad Reza Esmaeili, Ozrasadat Asadi, Fatemeh Ahmadinia, Hassan Shamsoddini, Banafshe Abbasifard, Mitra Implications of Peptidyl Arginine Deiminase 4 gene transcription and polymorphisms in susceptibility to rheumatoid arthritis in an Iranian population |
title | Implications of Peptidyl Arginine Deiminase 4 gene transcription and polymorphisms in susceptibility to rheumatoid arthritis in an Iranian population |
title_full | Implications of Peptidyl Arginine Deiminase 4 gene transcription and polymorphisms in susceptibility to rheumatoid arthritis in an Iranian population |
title_fullStr | Implications of Peptidyl Arginine Deiminase 4 gene transcription and polymorphisms in susceptibility to rheumatoid arthritis in an Iranian population |
title_full_unstemmed | Implications of Peptidyl Arginine Deiminase 4 gene transcription and polymorphisms in susceptibility to rheumatoid arthritis in an Iranian population |
title_short | Implications of Peptidyl Arginine Deiminase 4 gene transcription and polymorphisms in susceptibility to rheumatoid arthritis in an Iranian population |
title_sort | implications of peptidyl arginine deiminase 4 gene transcription and polymorphisms in susceptibility to rheumatoid arthritis in an iranian population |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10186752/ https://www.ncbi.nlm.nih.gov/pubmed/37193992 http://dx.doi.org/10.1186/s12920-023-01532-9 |
work_keys_str_mv | AT bagherihosseinabadizahra implicationsofpeptidylargininedeiminase4genetranscriptionandpolymorphismsinsusceptibilitytorheumatoidarthritisinaniranianpopulation AT mirzaeimohammadreza implicationsofpeptidylargininedeiminase4genetranscriptionandpolymorphismsinsusceptibilitytorheumatoidarthritisinaniranianpopulation AT esmaeiliozrasadat implicationsofpeptidylargininedeiminase4genetranscriptionandpolymorphismsinsusceptibilitytorheumatoidarthritisinaniranianpopulation AT asadifatemeh implicationsofpeptidylargininedeiminase4genetranscriptionandpolymorphismsinsusceptibilitytorheumatoidarthritisinaniranianpopulation AT ahmadiniahassan implicationsofpeptidylargininedeiminase4genetranscriptionandpolymorphismsinsusceptibilitytorheumatoidarthritisinaniranianpopulation AT shamsoddinibanafshe implicationsofpeptidylargininedeiminase4genetranscriptionandpolymorphismsinsusceptibilitytorheumatoidarthritisinaniranianpopulation AT abbasifardmitra implicationsofpeptidylargininedeiminase4genetranscriptionandpolymorphismsinsusceptibilitytorheumatoidarthritisinaniranianpopulation |