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Orthogonal dual reporter-based gain-of-signal assay for probing SARS-CoV-2 3CL protease activity in living cells: inhibitor identification and mutation investigation

The main protease (3-chymotrypsin-like protease, 3CLpro) of SARS-CoV-2 has become a focus of anti-coronavirus research. Despite efforts, drug development targeting 3CLpro has been hampered by limitations in the currently available activity assays. Additionally, the emergence of 3CLpro mutations in c...

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Detalles Bibliográficos
Autores principales:	Bei, Zhu-Chun, Yu, Huanhuan, Wang, Hong, Li, Qingyun, Wang, Baogang, Zhang, Dongna, Xu, Likun, Zhao, Liangliang, Dong, Shuwei, Song, Yabin
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Taylor & Francis 2023
Materias:	Coronaviruses
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10187092/ https://www.ncbi.nlm.nih.gov/pubmed/37144395 http://dx.doi.org/10.1080/22221751.2023.2211688

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