Common Analysis of Direct RNA SequencinG CUrrently Leads to Misidentification of 5-Methylcytosine Modifications at GCU Motifs

RNA modifications, such as méthylation, can be detected with Oxford Nanopore Technologies direct RNA sequencing. One commonly used tool for detecting 5-methylcytosine (m(5)C) modifications is Tombo, which uses an “Alternative Model” to detect putative modifications from a single sample. We examined...

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Detalles Bibliográficos
Autores principales: Watson, Kaylee J., Bromley, Robin E., Sparklin, Benjamin C., Gasser, Mark T., Bhattacharya, Tamanash, Lebov, Jarrett F., Tyson, Tyonna, Teigen, Laura E., Graf, Karen T., Michalski, Michelle, Bruno, Vincent M., Lindsey, Amelia R. I., Hardy, Richard W., Newton, Irene L. G., Hotopp, Julie C. Dunning
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10187288/
https://www.ncbi.nlm.nih.gov/pubmed/37205495
http://dx.doi.org/10.1101/2023.05.03.539298

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