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Integrative multi-dimensional characterization of striatal projection neuron heterogeneity in adult brain
Striatal projection neurons (SPNs) are traditionally segregated into two subpopulations expressing dopamine (DA) D(1)-like or D(2)-like receptors. However, this dichotomy is challenged by recent evidence. Functional and expression studies raise important questions: do SPNs co-express different DA re...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Cold Spring Harbor Laboratory
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10187292/ https://www.ncbi.nlm.nih.gov/pubmed/37205475 http://dx.doi.org/10.1101/2023.05.04.539488 |
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author | Gayden, Jenesis Puig, Stephanie Srinivasan, Chaitanya Phan, BaDoi N. Abdelhady, Ghada Buck, Silas A. Gamble, Mackenzie C. Tejeda, Hugo A. Dong, Yan Pfenning, Andreas R. Logan, Ryan W. Freyberg, Zachary |
author_facet | Gayden, Jenesis Puig, Stephanie Srinivasan, Chaitanya Phan, BaDoi N. Abdelhady, Ghada Buck, Silas A. Gamble, Mackenzie C. Tejeda, Hugo A. Dong, Yan Pfenning, Andreas R. Logan, Ryan W. Freyberg, Zachary |
author_sort | Gayden, Jenesis |
collection | PubMed |
description | Striatal projection neurons (SPNs) are traditionally segregated into two subpopulations expressing dopamine (DA) D(1)-like or D(2)-like receptors. However, this dichotomy is challenged by recent evidence. Functional and expression studies raise important questions: do SPNs co-express different DA receptors, and do these differences reflect unique striatal spatial distributions and expression profiles? Using RNAscope in mouse striatum, we report heterogenous SPN subpopulations distributed across dorsal-ventral and rostral-caudal axes. SPN subpopulations co-express multiple DA receptors, including D(1) and D(2) (D1/2R) and D(1) and D(3). Our integrative approach using single-nuclei multi-omics analyses provides a simple consensus to describe SPNs across diverse datasets, connecting it to complementary spatial mapping. Combining RNAscope and multi-omics shows D1/2R SPNs further separate into distinct subtypes according to spatial organization and conserved marker genes. Each SPN cell type contributes uniquely to genetic risk for neuropsychiatric diseases. Our results bridge anatomy and transcriptomics to offer new understandings of striatal neuron heterogeneity. |
format | Online Article Text |
id | pubmed-10187292 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Cold Spring Harbor Laboratory |
record_format | MEDLINE/PubMed |
spelling | pubmed-101872922023-05-17 Integrative multi-dimensional characterization of striatal projection neuron heterogeneity in adult brain Gayden, Jenesis Puig, Stephanie Srinivasan, Chaitanya Phan, BaDoi N. Abdelhady, Ghada Buck, Silas A. Gamble, Mackenzie C. Tejeda, Hugo A. Dong, Yan Pfenning, Andreas R. Logan, Ryan W. Freyberg, Zachary bioRxiv Article Striatal projection neurons (SPNs) are traditionally segregated into two subpopulations expressing dopamine (DA) D(1)-like or D(2)-like receptors. However, this dichotomy is challenged by recent evidence. Functional and expression studies raise important questions: do SPNs co-express different DA receptors, and do these differences reflect unique striatal spatial distributions and expression profiles? Using RNAscope in mouse striatum, we report heterogenous SPN subpopulations distributed across dorsal-ventral and rostral-caudal axes. SPN subpopulations co-express multiple DA receptors, including D(1) and D(2) (D1/2R) and D(1) and D(3). Our integrative approach using single-nuclei multi-omics analyses provides a simple consensus to describe SPNs across diverse datasets, connecting it to complementary spatial mapping. Combining RNAscope and multi-omics shows D1/2R SPNs further separate into distinct subtypes according to spatial organization and conserved marker genes. Each SPN cell type contributes uniquely to genetic risk for neuropsychiatric diseases. Our results bridge anatomy and transcriptomics to offer new understandings of striatal neuron heterogeneity. Cold Spring Harbor Laboratory 2023-10-21 /pmc/articles/PMC10187292/ /pubmed/37205475 http://dx.doi.org/10.1101/2023.05.04.539488 Text en https://creativecommons.org/licenses/by-nc-nd/4.0/This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which allows reusers to copy and distribute the material in any medium or format in unadapted form only, for noncommercial purposes only, and only so long as attribution is given to the creator. |
spellingShingle | Article Gayden, Jenesis Puig, Stephanie Srinivasan, Chaitanya Phan, BaDoi N. Abdelhady, Ghada Buck, Silas A. Gamble, Mackenzie C. Tejeda, Hugo A. Dong, Yan Pfenning, Andreas R. Logan, Ryan W. Freyberg, Zachary Integrative multi-dimensional characterization of striatal projection neuron heterogeneity in adult brain |
title | Integrative multi-dimensional characterization of striatal projection neuron heterogeneity in adult brain |
title_full | Integrative multi-dimensional characterization of striatal projection neuron heterogeneity in adult brain |
title_fullStr | Integrative multi-dimensional characterization of striatal projection neuron heterogeneity in adult brain |
title_full_unstemmed | Integrative multi-dimensional characterization of striatal projection neuron heterogeneity in adult brain |
title_short | Integrative multi-dimensional characterization of striatal projection neuron heterogeneity in adult brain |
title_sort | integrative multi-dimensional characterization of striatal projection neuron heterogeneity in adult brain |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10187292/ https://www.ncbi.nlm.nih.gov/pubmed/37205475 http://dx.doi.org/10.1101/2023.05.04.539488 |
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