Calcium-triggered DNA-mediated membrane fusion in synthetic cells

In cells, membrane fusion is mediated by SNARE proteins, whose activities are calcium-dependent. While several non-native membrane fusion mechanisms have been demonstrated, few can respond to external stimuli. Here, we develop a calcium-triggered DNA-mediated membrane fusion strategy where fusion is...

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Detalles Bibliográficos
Autores principales: Hsu, Yen-Yu, Chen, Samuel J., Bernal-Chanchavac, Julio, Sharma, Bineet, Moghimianavval, Hossein, Stephanopoulos, Nicholas, Liu, Allen P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10187331/
https://www.ncbi.nlm.nih.gov/pubmed/37205334
http://dx.doi.org/10.1101/2023.05.06.539684
Descripción
Sumario:In cells, membrane fusion is mediated by SNARE proteins, whose activities are calcium-dependent. While several non-native membrane fusion mechanisms have been demonstrated, few can respond to external stimuli. Here, we develop a calcium-triggered DNA-mediated membrane fusion strategy where fusion is regulated using surface-bound PEG chains that are cleavable by the calcium-activated protease calpain-1.

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