Calcium-triggered DNA-mediated membrane fusion in synthetic cells

In cells, membrane fusion is mediated by SNARE proteins, whose activities are calcium-dependent. While several non-native membrane fusion mechanisms have been demonstrated, few can respond to external stimuli. Here, we develop a calcium-triggered DNA-mediated membrane fusion strategy where fusion is...

Descripción completa

Detalles Bibliográficos
Autores principales: Hsu, Yen-Yu, Chen, Samuel J., Bernal-Chanchavac, Julio, Sharma, Bineet, Moghimianavval, Hossein, Stephanopoulos, Nicholas, Liu, Allen P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10187331/
https://www.ncbi.nlm.nih.gov/pubmed/37205334
http://dx.doi.org/10.1101/2023.05.06.539684
_version_ 1785042720406896640
author Hsu, Yen-Yu
Chen, Samuel J.
Bernal-Chanchavac, Julio
Sharma, Bineet
Moghimianavval, Hossein
Stephanopoulos, Nicholas
Liu, Allen P.
author_facet Hsu, Yen-Yu
Chen, Samuel J.
Bernal-Chanchavac, Julio
Sharma, Bineet
Moghimianavval, Hossein
Stephanopoulos, Nicholas
Liu, Allen P.
author_sort Hsu, Yen-Yu
collection PubMed
description In cells, membrane fusion is mediated by SNARE proteins, whose activities are calcium-dependent. While several non-native membrane fusion mechanisms have been demonstrated, few can respond to external stimuli. Here, we develop a calcium-triggered DNA-mediated membrane fusion strategy where fusion is regulated using surface-bound PEG chains that are cleavable by the calcium-activated protease calpain-1.
format Online
Article
Text
id pubmed-10187331
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Cold Spring Harbor Laboratory
record_format MEDLINE/PubMed
spelling pubmed-101873312023-05-17 Calcium-triggered DNA-mediated membrane fusion in synthetic cells Hsu, Yen-Yu Chen, Samuel J. Bernal-Chanchavac, Julio Sharma, Bineet Moghimianavval, Hossein Stephanopoulos, Nicholas Liu, Allen P. bioRxiv Article In cells, membrane fusion is mediated by SNARE proteins, whose activities are calcium-dependent. While several non-native membrane fusion mechanisms have been demonstrated, few can respond to external stimuli. Here, we develop a calcium-triggered DNA-mediated membrane fusion strategy where fusion is regulated using surface-bound PEG chains that are cleavable by the calcium-activated protease calpain-1. Cold Spring Harbor Laboratory 2023-06-02 /pmc/articles/PMC10187331/ /pubmed/37205334 http://dx.doi.org/10.1101/2023.05.06.539684 Text en https://creativecommons.org/licenses/by-nc-nd/4.0/This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which allows reusers to copy and distribute the material in any medium or format in unadapted form only, for noncommercial purposes only, and only so long as attribution is given to the creator.
spellingShingle Article
Hsu, Yen-Yu
Chen, Samuel J.
Bernal-Chanchavac, Julio
Sharma, Bineet
Moghimianavval, Hossein
Stephanopoulos, Nicholas
Liu, Allen P.
Calcium-triggered DNA-mediated membrane fusion in synthetic cells
title Calcium-triggered DNA-mediated membrane fusion in synthetic cells
title_full Calcium-triggered DNA-mediated membrane fusion in synthetic cells
title_fullStr Calcium-triggered DNA-mediated membrane fusion in synthetic cells
title_full_unstemmed Calcium-triggered DNA-mediated membrane fusion in synthetic cells
title_short Calcium-triggered DNA-mediated membrane fusion in synthetic cells
title_sort calcium-triggered dna-mediated membrane fusion in synthetic cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10187331/
https://www.ncbi.nlm.nih.gov/pubmed/37205334
http://dx.doi.org/10.1101/2023.05.06.539684
work_keys_str_mv AT hsuyenyu calciumtriggereddnamediatedmembranefusioninsyntheticcells
AT chensamuelj calciumtriggereddnamediatedmembranefusioninsyntheticcells
AT bernalchanchavacjulio calciumtriggereddnamediatedmembranefusioninsyntheticcells
AT sharmabineet calciumtriggereddnamediatedmembranefusioninsyntheticcells
AT moghimianavvalhossein calciumtriggereddnamediatedmembranefusioninsyntheticcells
AT stephanopoulosnicholas calciumtriggereddnamediatedmembranefusioninsyntheticcells
AT liuallenp calciumtriggereddnamediatedmembranefusioninsyntheticcells

Ejemplares similares