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Complement-dependent mpox-virus-neutralizing antibodies in infected and vaccinated individuals
Mpox virus (MPXV) caused a multi-country outbreak in non-endemic areas in 2022. Following historic success of smallpox vaccination with vaccinia virus (VACV)-based vaccines, the third generation modified vaccinia Ankara (MVA)-based vaccine was used as prophylaxis for MPXV, but its effectiveness rema...
| Autores principales: | , , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier Inc.
2023
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10188274/ https://www.ncbi.nlm.nih.gov/pubmed/37196656 http://dx.doi.org/10.1016/j.chom.2023.05.001 |
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| author | Hubert, Mathieu Guivel-Benhassine, Florence Bruel, Timothée Porrot, Françoise Planas, Delphine Vanhomwegen, Jessica Wiedemann, Aurélie Burrel, Sonia Marot, Stéphane Palich, Romain Monsel, Gentiane Diombera, Harouna Gallien, Sébastien Lopez-Zaragoza, Jose Luis Vindrios, William Taieb, Fabien Fernandes-Pellerin, Sandrine Delhaye, Maurine Laude, Hélène Arowas, Laurence Ungeheuer, Marie-Noelle Hocqueloux, Laurent Pourcher, Valérie Prazuck, Thierry Marcelin, Anne-Geneviève Lelièvre, Jean-Daniel Batéjat, Christophe Lévy, Yves Manuguerra, Jean-Claude Schwartz, Olivier |
| author_facet | Hubert, Mathieu Guivel-Benhassine, Florence Bruel, Timothée Porrot, Françoise Planas, Delphine Vanhomwegen, Jessica Wiedemann, Aurélie Burrel, Sonia Marot, Stéphane Palich, Romain Monsel, Gentiane Diombera, Harouna Gallien, Sébastien Lopez-Zaragoza, Jose Luis Vindrios, William Taieb, Fabien Fernandes-Pellerin, Sandrine Delhaye, Maurine Laude, Hélène Arowas, Laurence Ungeheuer, Marie-Noelle Hocqueloux, Laurent Pourcher, Valérie Prazuck, Thierry Marcelin, Anne-Geneviève Lelièvre, Jean-Daniel Batéjat, Christophe Lévy, Yves Manuguerra, Jean-Claude Schwartz, Olivier |
| author_sort | Hubert, Mathieu |
| collection | PubMed |
| description | Mpox virus (MPXV) caused a multi-country outbreak in non-endemic areas in 2022. Following historic success of smallpox vaccination with vaccinia virus (VACV)-based vaccines, the third generation modified vaccinia Ankara (MVA)-based vaccine was used as prophylaxis for MPXV, but its effectiveness remains poorly characterized. Here, we applied two assays to quantify neutralizing antibodies (NAbs) in sera from control, MPXV-infected, or MVA-vaccinated individuals. Various levels of MVA NAbs were detected after infection, historic smallpox, or recent MVA vaccination. MPXV was minimally sensitive to neutralization. However, addition of complement enhanced detection of responsive individuals and NAb levels. Anti-MVA and -MPXV NAbs were observed in 94% and 82% of infected individuals, respectively, and 92% and 56% of MVA vaccinees, respectively. NAb titers were higher in individuals born before 1980, highlighting the impact of historic smallpox vaccination on humoral immunity. Altogether, our results indicate that MPXV neutralization is complement dependent and uncover mechanisms underlying vaccine effectiveness. |
| format | Online Article Text |
| id | pubmed-10188274 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2023 |
| publisher | Elsevier Inc. |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-101882742023-05-17 Complement-dependent mpox-virus-neutralizing antibodies in infected and vaccinated individuals Hubert, Mathieu Guivel-Benhassine, Florence Bruel, Timothée Porrot, Françoise Planas, Delphine Vanhomwegen, Jessica Wiedemann, Aurélie Burrel, Sonia Marot, Stéphane Palich, Romain Monsel, Gentiane Diombera, Harouna Gallien, Sébastien Lopez-Zaragoza, Jose Luis Vindrios, William Taieb, Fabien Fernandes-Pellerin, Sandrine Delhaye, Maurine Laude, Hélène Arowas, Laurence Ungeheuer, Marie-Noelle Hocqueloux, Laurent Pourcher, Valérie Prazuck, Thierry Marcelin, Anne-Geneviève Lelièvre, Jean-Daniel Batéjat, Christophe Lévy, Yves Manuguerra, Jean-Claude Schwartz, Olivier Cell Host Microbe Article Mpox virus (MPXV) caused a multi-country outbreak in non-endemic areas in 2022. Following historic success of smallpox vaccination with vaccinia virus (VACV)-based vaccines, the third generation modified vaccinia Ankara (MVA)-based vaccine was used as prophylaxis for MPXV, but its effectiveness remains poorly characterized. Here, we applied two assays to quantify neutralizing antibodies (NAbs) in sera from control, MPXV-infected, or MVA-vaccinated individuals. Various levels of MVA NAbs were detected after infection, historic smallpox, or recent MVA vaccination. MPXV was minimally sensitive to neutralization. However, addition of complement enhanced detection of responsive individuals and NAb levels. Anti-MVA and -MPXV NAbs were observed in 94% and 82% of infected individuals, respectively, and 92% and 56% of MVA vaccinees, respectively. NAb titers were higher in individuals born before 1980, highlighting the impact of historic smallpox vaccination on humoral immunity. Altogether, our results indicate that MPXV neutralization is complement dependent and uncover mechanisms underlying vaccine effectiveness. Elsevier Inc. 2023-06-14 2023-05-05 /pmc/articles/PMC10188274/ /pubmed/37196656 http://dx.doi.org/10.1016/j.chom.2023.05.001 Text en © 2023 Elsevier Inc. Elsevier has created a Monkeypox Information Center (https://www.elsevier.com/connect/monkeypox-information-center) in response to the declared public health emergency of international concern, with free information in English on the monkeypox virus. The Monkeypox Information Center is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its monkeypox related research that is available on the Monkeypox Information Center - including this research content - immediately available in publicly funded repositories, with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the Monkeypox Information Center remains active. |
| spellingShingle | Article Hubert, Mathieu Guivel-Benhassine, Florence Bruel, Timothée Porrot, Françoise Planas, Delphine Vanhomwegen, Jessica Wiedemann, Aurélie Burrel, Sonia Marot, Stéphane Palich, Romain Monsel, Gentiane Diombera, Harouna Gallien, Sébastien Lopez-Zaragoza, Jose Luis Vindrios, William Taieb, Fabien Fernandes-Pellerin, Sandrine Delhaye, Maurine Laude, Hélène Arowas, Laurence Ungeheuer, Marie-Noelle Hocqueloux, Laurent Pourcher, Valérie Prazuck, Thierry Marcelin, Anne-Geneviève Lelièvre, Jean-Daniel Batéjat, Christophe Lévy, Yves Manuguerra, Jean-Claude Schwartz, Olivier Complement-dependent mpox-virus-neutralizing antibodies in infected and vaccinated individuals |
| title | Complement-dependent mpox-virus-neutralizing antibodies in infected and vaccinated individuals |
| title_full | Complement-dependent mpox-virus-neutralizing antibodies in infected and vaccinated individuals |
| title_fullStr | Complement-dependent mpox-virus-neutralizing antibodies in infected and vaccinated individuals |
| title_full_unstemmed | Complement-dependent mpox-virus-neutralizing antibodies in infected and vaccinated individuals |
| title_short | Complement-dependent mpox-virus-neutralizing antibodies in infected and vaccinated individuals |
| title_sort | complement-dependent mpox-virus-neutralizing antibodies in infected and vaccinated individuals |
| topic | Article |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10188274/ https://www.ncbi.nlm.nih.gov/pubmed/37196656 http://dx.doi.org/10.1016/j.chom.2023.05.001 |
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