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Heat shock factor 5 establishes the male germ-line meiotic sex chromosome inactivation through regulation of Smarca4

Meiotic sex chromosome inactivation is an essential event in male germ cell development, which is directed by DNA damage response signaling independent of Xist RNA to silence the transcription activity of the sex chromosomes. However, the specific mechanism of establishment and maintenance of meioti...

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Detalles Bibliográficos
Autores principales: Barutc, A Rasim, Frit, Andrew J., McCor, Rachel P., Nick, Jeffrey A., Asla, Muhammad
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10189179/
https://www.ncbi.nlm.nih.gov/pubmed/37206036
http://dx.doi.org/10.1016/j.heliyon.2023.e15194
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author Barutc, A Rasim
Frit, Andrew J.
McCor, Rachel P.
Nick, Jeffrey A.
Asla, Muhammad
author_facet Barutc, A Rasim
Frit, Andrew J.
McCor, Rachel P.
Nick, Jeffrey A.
Asla, Muhammad
author_sort Barutc, A Rasim
collection PubMed
description Meiotic sex chromosome inactivation is an essential event in male germ cell development, which is directed by DNA damage response signaling independent of Xist RNA to silence the transcription activity of the sex chromosomes. However, the specific mechanism of establishment and maintenance of meiotic chromosome silencing is still unclear. Here we identify the HSF5 as a testicular specific protein and the expression of which was at the onset of meiosis pachytene stage to round sperm. When the function of the HSF5 was lost, meiosis sex chromosome remodeling and silencing fail, followed by activation of CHK2 checkpoint leads to germ cell apoptosis. Furthermore, we found that SMARCA4 in the linking the HSF5 to MSCI and uncover additional factors with meiotic sex chromosome remodeling. Together, our results demonstrate a requirement for HSF5 activity in spermatogenesis and suggest a role for the mammalian HSF5-SMARCA4 in programmed meiotic sex chromosome remodeling and silencing events that take place during meiosis.
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spelling pubmed-101891792023-05-18 Heat shock factor 5 establishes the male germ-line meiotic sex chromosome inactivation through regulation of Smarca4 Barutc, A Rasim Frit, Andrew J. McCor, Rachel P. Nick, Jeffrey A. Asla, Muhammad Heliyon Research Article Meiotic sex chromosome inactivation is an essential event in male germ cell development, which is directed by DNA damage response signaling independent of Xist RNA to silence the transcription activity of the sex chromosomes. However, the specific mechanism of establishment and maintenance of meiotic chromosome silencing is still unclear. Here we identify the HSF5 as a testicular specific protein and the expression of which was at the onset of meiosis pachytene stage to round sperm. When the function of the HSF5 was lost, meiosis sex chromosome remodeling and silencing fail, followed by activation of CHK2 checkpoint leads to germ cell apoptosis. Furthermore, we found that SMARCA4 in the linking the HSF5 to MSCI and uncover additional factors with meiotic sex chromosome remodeling. Together, our results demonstrate a requirement for HSF5 activity in spermatogenesis and suggest a role for the mammalian HSF5-SMARCA4 in programmed meiotic sex chromosome remodeling and silencing events that take place during meiosis. Elsevier 2023-04-21 /pmc/articles/PMC10189179/ /pubmed/37206036 http://dx.doi.org/10.1016/j.heliyon.2023.e15194 Text en © 2023 Published by Elsevier Ltd. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Research Article
Barutc, A Rasim
Frit, Andrew J.
McCor, Rachel P.
Nick, Jeffrey A.
Asla, Muhammad
Heat shock factor 5 establishes the male germ-line meiotic sex chromosome inactivation through regulation of Smarca4
title Heat shock factor 5 establishes the male germ-line meiotic sex chromosome inactivation through regulation of Smarca4
title_full Heat shock factor 5 establishes the male germ-line meiotic sex chromosome inactivation through regulation of Smarca4
title_fullStr Heat shock factor 5 establishes the male germ-line meiotic sex chromosome inactivation through regulation of Smarca4
title_full_unstemmed Heat shock factor 5 establishes the male germ-line meiotic sex chromosome inactivation through regulation of Smarca4
title_short Heat shock factor 5 establishes the male germ-line meiotic sex chromosome inactivation through regulation of Smarca4
title_sort heat shock factor 5 establishes the male germ-line meiotic sex chromosome inactivation through regulation of smarca4
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10189179/
https://www.ncbi.nlm.nih.gov/pubmed/37206036
http://dx.doi.org/10.1016/j.heliyon.2023.e15194
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