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Time-dependent extracellular matrix alterations of young tendons in response to stress relaxation: a model for the Ponseti method
The Ponseti method corrects a clubfoot by manipulation and casting which causes stress relaxation on the tendons. Here, we examined the effect of long-term stress relaxation on tendon extracellular matrix (ECM) by (1) an ex vivo stress relaxation test, (2) an in vitro tenocyte culture with stress re...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Royal Society
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10189311/ https://www.ncbi.nlm.nih.gov/pubmed/37194273 http://dx.doi.org/10.1098/rsif.2022.0712 |
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author | Lee, Mu-Huan Tsai, Hung-Pei Lavy, Chris Mouthuy, Pierre-Alexis Czernuszka, Jan |
author_facet | Lee, Mu-Huan Tsai, Hung-Pei Lavy, Chris Mouthuy, Pierre-Alexis Czernuszka, Jan |
author_sort | Lee, Mu-Huan |
collection | PubMed |
description | The Ponseti method corrects a clubfoot by manipulation and casting which causes stress relaxation on the tendons. Here, we examined the effect of long-term stress relaxation on tendon extracellular matrix (ECM) by (1) an ex vivo stress relaxation test, (2) an in vitro tenocyte culture with stress relaxation and (3) an in vivo rabbit study. Time-dependent tendon lengthening and ECM alterations including crimp angle reduction and cleaved elastin were observed, which illustrated the mechanism of tissue lengthening behind the treatment—a material-based crimp angle reduction resulted from elastin cleavage. Additionally, in vitro and in vivo results observed restoration of these ECM alterations along with increased elastin level after 7 days of treatment, and the existence of neovascularization and inflammation, indicating the recovery and adaptation from the tendon in reaction to the treatment. Overall, this study provides the scientific background and information that helps explain the Ponseti method. |
format | Online Article Text |
id | pubmed-10189311 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | The Royal Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-101893112023-05-18 Time-dependent extracellular matrix alterations of young tendons in response to stress relaxation: a model for the Ponseti method Lee, Mu-Huan Tsai, Hung-Pei Lavy, Chris Mouthuy, Pierre-Alexis Czernuszka, Jan J R Soc Interface Life Sciences–Engineering interface The Ponseti method corrects a clubfoot by manipulation and casting which causes stress relaxation on the tendons. Here, we examined the effect of long-term stress relaxation on tendon extracellular matrix (ECM) by (1) an ex vivo stress relaxation test, (2) an in vitro tenocyte culture with stress relaxation and (3) an in vivo rabbit study. Time-dependent tendon lengthening and ECM alterations including crimp angle reduction and cleaved elastin were observed, which illustrated the mechanism of tissue lengthening behind the treatment—a material-based crimp angle reduction resulted from elastin cleavage. Additionally, in vitro and in vivo results observed restoration of these ECM alterations along with increased elastin level after 7 days of treatment, and the existence of neovascularization and inflammation, indicating the recovery and adaptation from the tendon in reaction to the treatment. Overall, this study provides the scientific background and information that helps explain the Ponseti method. The Royal Society 2023-05-17 /pmc/articles/PMC10189311/ /pubmed/37194273 http://dx.doi.org/10.1098/rsif.2022.0712 Text en © 2023 The Authors. https://creativecommons.org/licenses/by/4.0/Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, provided the original author and source are credited. |
spellingShingle | Life Sciences–Engineering interface Lee, Mu-Huan Tsai, Hung-Pei Lavy, Chris Mouthuy, Pierre-Alexis Czernuszka, Jan Time-dependent extracellular matrix alterations of young tendons in response to stress relaxation: a model for the Ponseti method |
title | Time-dependent extracellular matrix alterations of young tendons in response to stress relaxation: a model for the Ponseti method |
title_full | Time-dependent extracellular matrix alterations of young tendons in response to stress relaxation: a model for the Ponseti method |
title_fullStr | Time-dependent extracellular matrix alterations of young tendons in response to stress relaxation: a model for the Ponseti method |
title_full_unstemmed | Time-dependent extracellular matrix alterations of young tendons in response to stress relaxation: a model for the Ponseti method |
title_short | Time-dependent extracellular matrix alterations of young tendons in response to stress relaxation: a model for the Ponseti method |
title_sort | time-dependent extracellular matrix alterations of young tendons in response to stress relaxation: a model for the ponseti method |
topic | Life Sciences–Engineering interface |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10189311/ https://www.ncbi.nlm.nih.gov/pubmed/37194273 http://dx.doi.org/10.1098/rsif.2022.0712 |
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