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pH‐sustaining nanostructured hydroxyapatite/alginate composite hydrogel for gastric protection and intestinal release of Lactobacillus rhamnosus GG

The gut microbiome is closely linked to gastrointestinal health and disease status. Oral administration of known probiotic strains is now considered a promising therapeutic strategy, especially for refractory diseases such as inflammatory bowel disease. In this study, we developed a nanostructured h...

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Autores principales: Kim, Jihyun, Hlaing, Shwe Phyu, Lee, Juho, Kwak, Dongmin, Kim, Hyunwoo, Saparbayeva, Aruzhan, Yoon, In‐Soo, Im, Eunok, Jung, Yunjin, Yoo, Jin‐Wook
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley & Sons, Inc. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10189427/
https://www.ncbi.nlm.nih.gov/pubmed/37206214
http://dx.doi.org/10.1002/btm2.10527
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author Kim, Jihyun
Hlaing, Shwe Phyu
Lee, Juho
Kwak, Dongmin
Kim, Hyunwoo
Saparbayeva, Aruzhan
Yoon, In‐Soo
Im, Eunok
Jung, Yunjin
Yoo, Jin‐Wook
author_facet Kim, Jihyun
Hlaing, Shwe Phyu
Lee, Juho
Kwak, Dongmin
Kim, Hyunwoo
Saparbayeva, Aruzhan
Yoon, In‐Soo
Im, Eunok
Jung, Yunjin
Yoo, Jin‐Wook
author_sort Kim, Jihyun
collection PubMed
description The gut microbiome is closely linked to gastrointestinal health and disease status. Oral administration of known probiotic strains is now considered a promising therapeutic strategy, especially for refractory diseases such as inflammatory bowel disease. In this study, we developed a nanostructured hydroxyapatite/alginate (HAp/Alg) composite hydrogel that protects its encapsulated probiotic Lactobacillus rhamnosus GG (LGG) by neutralizing hydrogen ions that penetrate the hydrogel in a stomach without inhibiting LGG release in an intestine. Surface and transection analyses of the hydrogel revealed characteristic patterns of crystallization and composite‐layer formation. TEM revealed the dispersal of the nanosized HAp crystals and encapsulated LGG in the Alg hydrogel networks. The HAp/Alg composite hydrogel maintained its internal microenvironmental pH, thereby enabling the LGG to survive for substantially longer. At intestinal pH, the encapsulated LGG was completely released upon disintegration of the composite hydrogel. In a dextran sulfate sodium‐induced colitis mouse model, we then assessed the therapeutic effect of the LGG‐encapsulating hydrogel. This achieved intestinal delivery of LGG with minimal loss of enzymatic function and viability, ameliorating colitis by reducing epithelial damage, submucosal edema, inflammatory cell infiltration, and the number of goblet cells. These findings reveal the HAp/Alg composite hydrogel as a promising intestinal‐delivery platform for live microorganisms including probiotics and live biotherapeutic products.
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spelling pubmed-101894272023-05-18 pH‐sustaining nanostructured hydroxyapatite/alginate composite hydrogel for gastric protection and intestinal release of Lactobacillus rhamnosus GG Kim, Jihyun Hlaing, Shwe Phyu Lee, Juho Kwak, Dongmin Kim, Hyunwoo Saparbayeva, Aruzhan Yoon, In‐Soo Im, Eunok Jung, Yunjin Yoo, Jin‐Wook Bioeng Transl Med Research Articles The gut microbiome is closely linked to gastrointestinal health and disease status. Oral administration of known probiotic strains is now considered a promising therapeutic strategy, especially for refractory diseases such as inflammatory bowel disease. In this study, we developed a nanostructured hydroxyapatite/alginate (HAp/Alg) composite hydrogel that protects its encapsulated probiotic Lactobacillus rhamnosus GG (LGG) by neutralizing hydrogen ions that penetrate the hydrogel in a stomach without inhibiting LGG release in an intestine. Surface and transection analyses of the hydrogel revealed characteristic patterns of crystallization and composite‐layer formation. TEM revealed the dispersal of the nanosized HAp crystals and encapsulated LGG in the Alg hydrogel networks. The HAp/Alg composite hydrogel maintained its internal microenvironmental pH, thereby enabling the LGG to survive for substantially longer. At intestinal pH, the encapsulated LGG was completely released upon disintegration of the composite hydrogel. In a dextran sulfate sodium‐induced colitis mouse model, we then assessed the therapeutic effect of the LGG‐encapsulating hydrogel. This achieved intestinal delivery of LGG with minimal loss of enzymatic function and viability, ameliorating colitis by reducing epithelial damage, submucosal edema, inflammatory cell infiltration, and the number of goblet cells. These findings reveal the HAp/Alg composite hydrogel as a promising intestinal‐delivery platform for live microorganisms including probiotics and live biotherapeutic products. John Wiley & Sons, Inc. 2023-04-19 /pmc/articles/PMC10189427/ /pubmed/37206214 http://dx.doi.org/10.1002/btm2.10527 Text en © 2023 The Authors. Bioengineering & Translational Medicine published by Wiley Periodicals LLC on behalf of American Institute of Chemical Engineers. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Articles
Kim, Jihyun
Hlaing, Shwe Phyu
Lee, Juho
Kwak, Dongmin
Kim, Hyunwoo
Saparbayeva, Aruzhan
Yoon, In‐Soo
Im, Eunok
Jung, Yunjin
Yoo, Jin‐Wook
pH‐sustaining nanostructured hydroxyapatite/alginate composite hydrogel for gastric protection and intestinal release of Lactobacillus rhamnosus GG
title pH‐sustaining nanostructured hydroxyapatite/alginate composite hydrogel for gastric protection and intestinal release of Lactobacillus rhamnosus GG
title_full pH‐sustaining nanostructured hydroxyapatite/alginate composite hydrogel for gastric protection and intestinal release of Lactobacillus rhamnosus GG
title_fullStr pH‐sustaining nanostructured hydroxyapatite/alginate composite hydrogel for gastric protection and intestinal release of Lactobacillus rhamnosus GG
title_full_unstemmed pH‐sustaining nanostructured hydroxyapatite/alginate composite hydrogel for gastric protection and intestinal release of Lactobacillus rhamnosus GG
title_short pH‐sustaining nanostructured hydroxyapatite/alginate composite hydrogel for gastric protection and intestinal release of Lactobacillus rhamnosus GG
title_sort ph‐sustaining nanostructured hydroxyapatite/alginate composite hydrogel for gastric protection and intestinal release of lactobacillus rhamnosus gg
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10189427/
https://www.ncbi.nlm.nih.gov/pubmed/37206214
http://dx.doi.org/10.1002/btm2.10527
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