Cargando…

Collagen scaffolds derived from bovine skin loaded with MSC optimized M1 macrophages remodeling and chronic diabetic wounds healing

Owing to the persistent inflammatory microenvironment and unsubstantial dermal tissues, chronic diabetic wounds do not heal easily and their recurrence rate is high. Therefore, a dermal substitute that can induce rapid tissue regeneration and inhibit scar formation is urgently required to address th...

Descripción completa

Detalles Bibliográficos
Autores principales: Liu, Hengdeng, Yang, Ronghua, Zhao, Shixin, Zhou, Fei, Liu, Yiling, Zhou, Ziheng, Chen, Lei, Xie, Julin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley & Sons, Inc. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10189465/
https://www.ncbi.nlm.nih.gov/pubmed/37206210
http://dx.doi.org/10.1002/btm2.10467
_version_ 1785043093358116864
author Liu, Hengdeng
Yang, Ronghua
Zhao, Shixin
Zhou, Fei
Liu, Yiling
Zhou, Ziheng
Chen, Lei
Xie, Julin
author_facet Liu, Hengdeng
Yang, Ronghua
Zhao, Shixin
Zhou, Fei
Liu, Yiling
Zhou, Ziheng
Chen, Lei
Xie, Julin
author_sort Liu, Hengdeng
collection PubMed
description Owing to the persistent inflammatory microenvironment and unsubstantial dermal tissues, chronic diabetic wounds do not heal easily and their recurrence rate is high. Therefore, a dermal substitute that can induce rapid tissue regeneration and inhibit scar formation is urgently required to address this concern. In this study, we established biologically active dermal substitutes (BADS) by combining novel animal tissue‐derived collagen dermal‐replacement scaffolds (CDRS) and bone marrow mesenchymal stem cells (BMSCs) for the healing and recurrence treatments of chronic diabetic wounds. The collagen scaffolds derived from bovine skin (CBS) displayed good physicochemical properties and superior biocompatibility. CBS loaded with BMSCs (CBS‐MCSs) could inhibit M1 macrophage polarization in vitro. Decreased MMP‐9 and increased Col3 at the protein level were detected in CBS‐MSCs‐treated M1 macrophages, which may be attributed to the suppression of the TNF‐α/NF‐κB signaling pathway (downregulating phospho‐IKKα/β/total IKKα/β, phospho‐IκB/total IκB, and phospho‐NFκB/total NFκB) in M1 macrophages. Moreover, CBS‐MSCs could benefit the transformation of M1 (downregulating iNOS) to M2 (upregulating CD206) macrophages. Wound‐healing evaluations demonstrated that CBS‐MSCs regulated the polarization of macrophages and the balance of inflammatory factors (pro‐inflammatory: IL‐1β, TNF‐α, and MMP‐9; anti‐inflammatory: IL‐10 and TGF‐β3) in db/db mice. Furthermore, CBS‐MSCs facilitated the noncontractile and re‐epithelialized processes, granulation tissue regeneration, and neovascularization of chronic diabetic wounds. Thus, CBS‐MSCs have a potential value for clinical application in promoting the healing of chronic diabetic wounds and preventing the recurrence of ulcers.
format Online
Article
Text
id pubmed-10189465
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher John Wiley & Sons, Inc.
record_format MEDLINE/PubMed
spelling pubmed-101894652023-05-18 Collagen scaffolds derived from bovine skin loaded with MSC optimized M1 macrophages remodeling and chronic diabetic wounds healing Liu, Hengdeng Yang, Ronghua Zhao, Shixin Zhou, Fei Liu, Yiling Zhou, Ziheng Chen, Lei Xie, Julin Bioeng Transl Med Research Articles Owing to the persistent inflammatory microenvironment and unsubstantial dermal tissues, chronic diabetic wounds do not heal easily and their recurrence rate is high. Therefore, a dermal substitute that can induce rapid tissue regeneration and inhibit scar formation is urgently required to address this concern. In this study, we established biologically active dermal substitutes (BADS) by combining novel animal tissue‐derived collagen dermal‐replacement scaffolds (CDRS) and bone marrow mesenchymal stem cells (BMSCs) for the healing and recurrence treatments of chronic diabetic wounds. The collagen scaffolds derived from bovine skin (CBS) displayed good physicochemical properties and superior biocompatibility. CBS loaded with BMSCs (CBS‐MCSs) could inhibit M1 macrophage polarization in vitro. Decreased MMP‐9 and increased Col3 at the protein level were detected in CBS‐MSCs‐treated M1 macrophages, which may be attributed to the suppression of the TNF‐α/NF‐κB signaling pathway (downregulating phospho‐IKKα/β/total IKKα/β, phospho‐IκB/total IκB, and phospho‐NFκB/total NFκB) in M1 macrophages. Moreover, CBS‐MSCs could benefit the transformation of M1 (downregulating iNOS) to M2 (upregulating CD206) macrophages. Wound‐healing evaluations demonstrated that CBS‐MSCs regulated the polarization of macrophages and the balance of inflammatory factors (pro‐inflammatory: IL‐1β, TNF‐α, and MMP‐9; anti‐inflammatory: IL‐10 and TGF‐β3) in db/db mice. Furthermore, CBS‐MSCs facilitated the noncontractile and re‐epithelialized processes, granulation tissue regeneration, and neovascularization of chronic diabetic wounds. Thus, CBS‐MSCs have a potential value for clinical application in promoting the healing of chronic diabetic wounds and preventing the recurrence of ulcers. John Wiley & Sons, Inc. 2022-12-07 /pmc/articles/PMC10189465/ /pubmed/37206210 http://dx.doi.org/10.1002/btm2.10467 Text en © 2022 The Authors. Bioengineering & Translational Medicine published by Wiley Periodicals LLC on behalf of American Institute of Chemical Engineers. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Articles
Liu, Hengdeng
Yang, Ronghua
Zhao, Shixin
Zhou, Fei
Liu, Yiling
Zhou, Ziheng
Chen, Lei
Xie, Julin
Collagen scaffolds derived from bovine skin loaded with MSC optimized M1 macrophages remodeling and chronic diabetic wounds healing
title Collagen scaffolds derived from bovine skin loaded with MSC optimized M1 macrophages remodeling and chronic diabetic wounds healing
title_full Collagen scaffolds derived from bovine skin loaded with MSC optimized M1 macrophages remodeling and chronic diabetic wounds healing
title_fullStr Collagen scaffolds derived from bovine skin loaded with MSC optimized M1 macrophages remodeling and chronic diabetic wounds healing
title_full_unstemmed Collagen scaffolds derived from bovine skin loaded with MSC optimized M1 macrophages remodeling and chronic diabetic wounds healing
title_short Collagen scaffolds derived from bovine skin loaded with MSC optimized M1 macrophages remodeling and chronic diabetic wounds healing
title_sort collagen scaffolds derived from bovine skin loaded with msc optimized m1 macrophages remodeling and chronic diabetic wounds healing
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10189465/
https://www.ncbi.nlm.nih.gov/pubmed/37206210
http://dx.doi.org/10.1002/btm2.10467
work_keys_str_mv AT liuhengdeng collagenscaffoldsderivedfrombovineskinloadedwithmscoptimizedm1macrophagesremodelingandchronicdiabeticwoundshealing
AT yangronghua collagenscaffoldsderivedfrombovineskinloadedwithmscoptimizedm1macrophagesremodelingandchronicdiabeticwoundshealing
AT zhaoshixin collagenscaffoldsderivedfrombovineskinloadedwithmscoptimizedm1macrophagesremodelingandchronicdiabeticwoundshealing
AT zhoufei collagenscaffoldsderivedfrombovineskinloadedwithmscoptimizedm1macrophagesremodelingandchronicdiabeticwoundshealing
AT liuyiling collagenscaffoldsderivedfrombovineskinloadedwithmscoptimizedm1macrophagesremodelingandchronicdiabeticwoundshealing
AT zhouziheng collagenscaffoldsderivedfrombovineskinloadedwithmscoptimizedm1macrophagesremodelingandchronicdiabeticwoundshealing
AT chenlei collagenscaffoldsderivedfrombovineskinloadedwithmscoptimizedm1macrophagesremodelingandchronicdiabeticwoundshealing
AT xiejulin collagenscaffoldsderivedfrombovineskinloadedwithmscoptimizedm1macrophagesremodelingandchronicdiabeticwoundshealing