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Protocol for tumor dissociation and fluorescence-activated cell sorting of human head and neck cancers

Tumors originating from the head and neck represent diverse histologies and are comprised of several cell types, including malignant cells, cancer-associated fibroblasts, endothelial cells, and immune cells. In this protocol, we describe a step-by-step approach for the dissociation of fresh human he...

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Detalles Bibliográficos
Autores principales: Okolo, Ogoegbunam, Yu, Victoria, Flashner, Samuel, Martin, Cecilia, Nakagawa, Hiroshi, Lin, Derrick T., Puram, Sidharth V., Parikh, Anuraag S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10189548/
https://www.ncbi.nlm.nih.gov/pubmed/37149858
http://dx.doi.org/10.1016/j.xpro.2023.102294