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Protocol to generate endothelial cells, pericytes, and fibroblasts in one differentiation round from human-induced pluripotent stem cells

Here, we present a protocol for differentiating human-induced pluripotent stem cells into three distinct mesodermal cell types: vascular endothelial cells (ECs), pericytes, and fibroblasts. We describe steps for using monolayer serum-free differentiation and isolating ECs (CD31(+)) and mesenchymal p...

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Detalles Bibliográficos
Autores principales:	Park, Tea Soon, Hirday, Rishabh, Ali, Amir, Megersa, Roba, Villasmil, Rafael, Nguyen, Eric, Bharti, Kapil
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2023
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10189549/ https://www.ncbi.nlm.nih.gov/pubmed/37149860 http://dx.doi.org/10.1016/j.xpro.2023.102292

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