Protocol for electrotaxis of large epithelial cell sheets

Here, we present a protocol for electrotaxis of large epithelial cell sheets without compromising the integrity of cell epithelia in a high-throughput customized directed current electrotaxis chamber. We describe the fabrication and use of polydimethylsiloxane stencils to control the size and shape...

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Detalles Bibliográficos
Autores principales: Zhang, Yan, Lee, Rachel M., Zhu, Zijie, Sun, Yaohui, Zhu, Kan, Xu, Zhengping, Lin, Francis, Pan, Tingrui, Losert, Wolfgang, Zhao, Min
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10189550/
https://www.ncbi.nlm.nih.gov/pubmed/37149857
http://dx.doi.org/10.1016/j.xpro.2023.102288
Descripción
Sumario:Here, we present a protocol for electrotaxis of large epithelial cell sheets without compromising the integrity of cell epithelia in a high-throughput customized directed current electrotaxis chamber. We describe the fabrication and use of polydimethylsiloxane stencils to control the size and shape of human keratinocyte cell sheets. We detail cell tracking, cell sheet contour assay, and particle image velocimetry to reveal the spatial and temporal motility dynamics of cell sheets. This approach is applicable to other collective cell migration studies. For complete details on the use and execution of this protocol, please refer to Zhang et al. (2022).(1)

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