Protocol for electrotaxis of large epithelial cell sheets

Here, we present a protocol for electrotaxis of large epithelial cell sheets without compromising the integrity of cell epithelia in a high-throughput customized directed current electrotaxis chamber. We describe the fabrication and use of polydimethylsiloxane stencils to control the size and shape...

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Detalles Bibliográficos
Autores principales: Zhang, Yan, Lee, Rachel M., Zhu, Zijie, Sun, Yaohui, Zhu, Kan, Xu, Zhengping, Lin, Francis, Pan, Tingrui, Losert, Wolfgang, Zhao, Min
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10189550/
https://www.ncbi.nlm.nih.gov/pubmed/37149857
http://dx.doi.org/10.1016/j.xpro.2023.102288
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author Zhang, Yan
Lee, Rachel M.
Zhu, Zijie
Sun, Yaohui
Zhu, Kan
Xu, Zhengping
Lin, Francis
Pan, Tingrui
Losert, Wolfgang
Zhao, Min
author_facet Zhang, Yan
Lee, Rachel M.
Zhu, Zijie
Sun, Yaohui
Zhu, Kan
Xu, Zhengping
Lin, Francis
Pan, Tingrui
Losert, Wolfgang
Zhao, Min
author_sort Zhang, Yan
collection PubMed
description Here, we present a protocol for electrotaxis of large epithelial cell sheets without compromising the integrity of cell epithelia in a high-throughput customized directed current electrotaxis chamber. We describe the fabrication and use of polydimethylsiloxane stencils to control the size and shape of human keratinocyte cell sheets. We detail cell tracking, cell sheet contour assay, and particle image velocimetry to reveal the spatial and temporal motility dynamics of cell sheets. This approach is applicable to other collective cell migration studies. For complete details on the use and execution of this protocol, please refer to Zhang et al. (2022).(1)
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spelling pubmed-101895502023-05-18 Protocol for electrotaxis of large epithelial cell sheets Zhang, Yan Lee, Rachel M. Zhu, Zijie Sun, Yaohui Zhu, Kan Xu, Zhengping Lin, Francis Pan, Tingrui Losert, Wolfgang Zhao, Min STAR Protoc Protocol Here, we present a protocol for electrotaxis of large epithelial cell sheets without compromising the integrity of cell epithelia in a high-throughput customized directed current electrotaxis chamber. We describe the fabrication and use of polydimethylsiloxane stencils to control the size and shape of human keratinocyte cell sheets. We detail cell tracking, cell sheet contour assay, and particle image velocimetry to reveal the spatial and temporal motility dynamics of cell sheets. This approach is applicable to other collective cell migration studies. For complete details on the use and execution of this protocol, please refer to Zhang et al. (2022).(1) Elsevier 2023-05-06 /pmc/articles/PMC10189550/ /pubmed/37149857 http://dx.doi.org/10.1016/j.xpro.2023.102288 Text en © 2023 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Zhang, Yan
Lee, Rachel M.
Zhu, Zijie
Sun, Yaohui
Zhu, Kan
Xu, Zhengping
Lin, Francis
Pan, Tingrui
Losert, Wolfgang
Zhao, Min
Protocol for electrotaxis of large epithelial cell sheets
title Protocol for electrotaxis of large epithelial cell sheets
title_full Protocol for electrotaxis of large epithelial cell sheets
title_fullStr Protocol for electrotaxis of large epithelial cell sheets
title_full_unstemmed Protocol for electrotaxis of large epithelial cell sheets
title_short Protocol for electrotaxis of large epithelial cell sheets
title_sort protocol for electrotaxis of large epithelial cell sheets
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10189550/
https://www.ncbi.nlm.nih.gov/pubmed/37149857
http://dx.doi.org/10.1016/j.xpro.2023.102288
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