Cargando…

Attack of the clones: Population genetics reveals clonality of Colletotrichum lupini, the causal agent of lupin anthracnose

Colletotrichum lupini, the causative agent of lupin anthracnose, affects lupin cultivation worldwide. Understanding its population structure and evolutionary potential is crucial to design successful disease management strategies. The objective of this study was to employ population genetics to inve...

Descripción completa

Detalles Bibliográficos
Autores principales: Alkemade, Joris A., Baroncelli, Riccardo, Messmer, Monika M., Hohmann, Pierre
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10189766/
https://www.ncbi.nlm.nih.gov/pubmed/37078402
http://dx.doi.org/10.1111/mpp.13332
_version_ 1785043154667307008
author Alkemade, Joris A.
Baroncelli, Riccardo
Messmer, Monika M.
Hohmann, Pierre
author_facet Alkemade, Joris A.
Baroncelli, Riccardo
Messmer, Monika M.
Hohmann, Pierre
author_sort Alkemade, Joris A.
collection PubMed
description Colletotrichum lupini, the causative agent of lupin anthracnose, affects lupin cultivation worldwide. Understanding its population structure and evolutionary potential is crucial to design successful disease management strategies. The objective of this study was to employ population genetics to investigate the diversity, evolutionary dynamics, and molecular basis of the interaction of this notorious lupin pathogen with its host. A collection of globally representative C. lupini isolates was genotyped through triple digest restriction site‐associated DNA sequencing, resulting in a data set of unparalleled resolution. Phylogenetic and structural analysis could distinguish four independent lineages (I–IV). The strong population structure and high overall standardized index of association (r̅ (d)) indicates that C. lupini reproduces clonally. Different morphologies and virulence patterns on white lupin (Lupinus albus) and Andean lupin (Lupinus mutabilis) were observed between and within clonal lineages. Isolates belonging to lineage II were shown to have a minichromosome that was also partly present in lineage III and IV, but not in lineage I isolates. Variation in the presence of this minichromosome could imply a role in host–pathogen interaction. All four lineages were present in the South American Andes region, which is suggested to be the centre of origin of this species. Only members of lineage II have been found outside South America since the 1990s, indicating it as the current pandemic population. As a seedborne pathogen, C. lupini has mainly spread through infected but symptomless seeds, stressing the importance of phytosanitary measures to prevent future outbreaks of strains that are yet confined to South America.
format Online
Article
Text
id pubmed-10189766
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher John Wiley and Sons Inc.
record_format MEDLINE/PubMed
spelling pubmed-101897662023-05-18 Attack of the clones: Population genetics reveals clonality of Colletotrichum lupini, the causal agent of lupin anthracnose Alkemade, Joris A. Baroncelli, Riccardo Messmer, Monika M. Hohmann, Pierre Mol Plant Pathol Original Articles Colletotrichum lupini, the causative agent of lupin anthracnose, affects lupin cultivation worldwide. Understanding its population structure and evolutionary potential is crucial to design successful disease management strategies. The objective of this study was to employ population genetics to investigate the diversity, evolutionary dynamics, and molecular basis of the interaction of this notorious lupin pathogen with its host. A collection of globally representative C. lupini isolates was genotyped through triple digest restriction site‐associated DNA sequencing, resulting in a data set of unparalleled resolution. Phylogenetic and structural analysis could distinguish four independent lineages (I–IV). The strong population structure and high overall standardized index of association (r̅ (d)) indicates that C. lupini reproduces clonally. Different morphologies and virulence patterns on white lupin (Lupinus albus) and Andean lupin (Lupinus mutabilis) were observed between and within clonal lineages. Isolates belonging to lineage II were shown to have a minichromosome that was also partly present in lineage III and IV, but not in lineage I isolates. Variation in the presence of this minichromosome could imply a role in host–pathogen interaction. All four lineages were present in the South American Andes region, which is suggested to be the centre of origin of this species. Only members of lineage II have been found outside South America since the 1990s, indicating it as the current pandemic population. As a seedborne pathogen, C. lupini has mainly spread through infected but symptomless seeds, stressing the importance of phytosanitary measures to prevent future outbreaks of strains that are yet confined to South America. John Wiley and Sons Inc. 2023-04-20 /pmc/articles/PMC10189766/ /pubmed/37078402 http://dx.doi.org/10.1111/mpp.13332 Text en © 2023 The Authors. Molecular Plant Pathology published by British Society for Plant Pathology and John Wiley & Sons Ltd. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
Alkemade, Joris A.
Baroncelli, Riccardo
Messmer, Monika M.
Hohmann, Pierre
Attack of the clones: Population genetics reveals clonality of Colletotrichum lupini, the causal agent of lupin anthracnose
title Attack of the clones: Population genetics reveals clonality of Colletotrichum lupini, the causal agent of lupin anthracnose
title_full Attack of the clones: Population genetics reveals clonality of Colletotrichum lupini, the causal agent of lupin anthracnose
title_fullStr Attack of the clones: Population genetics reveals clonality of Colletotrichum lupini, the causal agent of lupin anthracnose
title_full_unstemmed Attack of the clones: Population genetics reveals clonality of Colletotrichum lupini, the causal agent of lupin anthracnose
title_short Attack of the clones: Population genetics reveals clonality of Colletotrichum lupini, the causal agent of lupin anthracnose
title_sort attack of the clones: population genetics reveals clonality of colletotrichum lupini, the causal agent of lupin anthracnose
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10189766/
https://www.ncbi.nlm.nih.gov/pubmed/37078402
http://dx.doi.org/10.1111/mpp.13332
work_keys_str_mv AT alkemadejorisa attackoftheclonespopulationgeneticsrevealsclonalityofcolletotrichumlupinithecausalagentoflupinanthracnose
AT baroncelliriccardo attackoftheclonespopulationgeneticsrevealsclonalityofcolletotrichumlupinithecausalagentoflupinanthracnose
AT messmermonikam attackoftheclonespopulationgeneticsrevealsclonalityofcolletotrichumlupinithecausalagentoflupinanthracnose
AT hohmannpierre attackoftheclonespopulationgeneticsrevealsclonalityofcolletotrichumlupinithecausalagentoflupinanthracnose