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Epigenome wide association study in peripheral blood of pregnant women identifies potential metabolic pathways related to gestational diabetes
Gestational diabetes mellitus (GDM) increases the risk of developing metabolic disorders in both pregnant women and their offspring. Factors such as nutrition or the intrauterine environment may play an important role, through epigenetic mechanisms, in the development of GDM. The aim of this work is...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Taylor & Francis
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10190180/ https://www.ncbi.nlm.nih.gov/pubmed/37192269 http://dx.doi.org/10.1080/15592294.2023.2211369 |
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author | Linares-Pineda, Teresa María Peña-Montero, Nerea Gutiérrez-Repiso, Carolina Lima-Rubio, Fuensanta Sánchez-Pozo, Antonio Tinahones, Francisco J Molina-Vega, María Picón-César, María José Morcillo, Sonsoles |
author_facet | Linares-Pineda, Teresa María Peña-Montero, Nerea Gutiérrez-Repiso, Carolina Lima-Rubio, Fuensanta Sánchez-Pozo, Antonio Tinahones, Francisco J Molina-Vega, María Picón-César, María José Morcillo, Sonsoles |
author_sort | Linares-Pineda, Teresa María |
collection | PubMed |
description | Gestational diabetes mellitus (GDM) increases the risk of developing metabolic disorders in both pregnant women and their offspring. Factors such as nutrition or the intrauterine environment may play an important role, through epigenetic mechanisms, in the development of GDM. The aim of this work is to identify epigenetic marks involved in the mechanisms or pathways related to gestational diabetes. A total of 32 pregnant women were selected, 16 of them with GDM and 16 non-GDM. DNA methylation pattern was obtained from Illumina Methylation Epic BeadChip, from peripheral blood samples at the diagnostic visit (26–28 weeks). Differential methylated positions (DMPs) were extracted using ChAMP and limma package in R 2.9.10, with a threshold of FDR <0.05, deltabeta >|5|% and B >0. A total of 1.141 DMPs were found, and 714 were annotated in genes. A functional analysis was performed, and we found 23 genes significantly related to carbohydrate metabolism. Finally, a total of 27 DMPs were correlated with biochemical variables such as glucose levels at different points of oral glucose tolerance test, fasting glucose, cholesterol, HOMAIR and HbA1c, at different visits during pregnancy and postpartum. Our results show that there is a differentiated methylation pattern between GDM and non-GDM. Furthermore, the genes annotated to the DMPs could be implicated in the development of GDM as well as in alterations in related metabolic variables. |
format | Online Article Text |
id | pubmed-10190180 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Taylor & Francis |
record_format | MEDLINE/PubMed |
spelling | pubmed-101901802023-05-18 Epigenome wide association study in peripheral blood of pregnant women identifies potential metabolic pathways related to gestational diabetes Linares-Pineda, Teresa María Peña-Montero, Nerea Gutiérrez-Repiso, Carolina Lima-Rubio, Fuensanta Sánchez-Pozo, Antonio Tinahones, Francisco J Molina-Vega, María Picón-César, María José Morcillo, Sonsoles Epigenetics Research Article Gestational diabetes mellitus (GDM) increases the risk of developing metabolic disorders in both pregnant women and their offspring. Factors such as nutrition or the intrauterine environment may play an important role, through epigenetic mechanisms, in the development of GDM. The aim of this work is to identify epigenetic marks involved in the mechanisms or pathways related to gestational diabetes. A total of 32 pregnant women were selected, 16 of them with GDM and 16 non-GDM. DNA methylation pattern was obtained from Illumina Methylation Epic BeadChip, from peripheral blood samples at the diagnostic visit (26–28 weeks). Differential methylated positions (DMPs) were extracted using ChAMP and limma package in R 2.9.10, with a threshold of FDR <0.05, deltabeta >|5|% and B >0. A total of 1.141 DMPs were found, and 714 were annotated in genes. A functional analysis was performed, and we found 23 genes significantly related to carbohydrate metabolism. Finally, a total of 27 DMPs were correlated with biochemical variables such as glucose levels at different points of oral glucose tolerance test, fasting glucose, cholesterol, HOMAIR and HbA1c, at different visits during pregnancy and postpartum. Our results show that there is a differentiated methylation pattern between GDM and non-GDM. Furthermore, the genes annotated to the DMPs could be implicated in the development of GDM as well as in alterations in related metabolic variables. Taylor & Francis 2023-05-16 /pmc/articles/PMC10190180/ /pubmed/37192269 http://dx.doi.org/10.1080/15592294.2023.2211369 Text en © 2023 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/) ), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The terms on which this article has been published allow the posting of the Accepted Manuscript in a repository by the author(s) or with their consent. |
spellingShingle | Research Article Linares-Pineda, Teresa María Peña-Montero, Nerea Gutiérrez-Repiso, Carolina Lima-Rubio, Fuensanta Sánchez-Pozo, Antonio Tinahones, Francisco J Molina-Vega, María Picón-César, María José Morcillo, Sonsoles Epigenome wide association study in peripheral blood of pregnant women identifies potential metabolic pathways related to gestational diabetes |
title | Epigenome wide association study in peripheral blood of pregnant women identifies potential metabolic pathways related to gestational diabetes |
title_full | Epigenome wide association study in peripheral blood of pregnant women identifies potential metabolic pathways related to gestational diabetes |
title_fullStr | Epigenome wide association study in peripheral blood of pregnant women identifies potential metabolic pathways related to gestational diabetes |
title_full_unstemmed | Epigenome wide association study in peripheral blood of pregnant women identifies potential metabolic pathways related to gestational diabetes |
title_short | Epigenome wide association study in peripheral blood of pregnant women identifies potential metabolic pathways related to gestational diabetes |
title_sort | epigenome wide association study in peripheral blood of pregnant women identifies potential metabolic pathways related to gestational diabetes |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10190180/ https://www.ncbi.nlm.nih.gov/pubmed/37192269 http://dx.doi.org/10.1080/15592294.2023.2211369 |
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