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Near-infrared imaging for automated tsetse pupae sex sorting in support of the sterile insect technique

Tsetse flies are the cyclical vectors of African trypanosomes and one of several methods to manage this vector is the sterile insect technique (SIT). The ability to determine the sex of tsetse pupae with the objective to separate the sexes before adult emergence has been a major goal for decades for...

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Autores principales: Argilés-Herrero, Rafael, Salvador-Herranz, Gustavo, Parker, Andrew G., Zacarés, Mario, Fall, Assane G., Gaye, Adji M., Nawaz, Arooj, Takáč, Peter, Vreysen, Marc J.B., de Beer, Chantel J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: EDP Sciences 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10191111/
https://www.ncbi.nlm.nih.gov/pubmed/37195141
http://dx.doi.org/10.1051/parasite/2023019
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author Argilés-Herrero, Rafael
Salvador-Herranz, Gustavo
Parker, Andrew G.
Zacarés, Mario
Fall, Assane G.
Gaye, Adji M.
Nawaz, Arooj
Takáč, Peter
Vreysen, Marc J.B.
de Beer, Chantel J.
author_facet Argilés-Herrero, Rafael
Salvador-Herranz, Gustavo
Parker, Andrew G.
Zacarés, Mario
Fall, Assane G.
Gaye, Adji M.
Nawaz, Arooj
Takáč, Peter
Vreysen, Marc J.B.
de Beer, Chantel J.
author_sort Argilés-Herrero, Rafael
collection PubMed
description Tsetse flies are the cyclical vectors of African trypanosomes and one of several methods to manage this vector is the sterile insect technique (SIT). The ability to determine the sex of tsetse pupae with the objective to separate the sexes before adult emergence has been a major goal for decades for tsetse management programmes with an SIT component. Tsetse females develop faster and pharate females inside the pupae melanise 1–2 days before males. This earlier melanisation can be detected by infrared cameras through the pupal shell, and the newly developed Near InfraRed Pupae Sex Sorter (NIRPSS) takes advantage of this. The melanisation process is not homogeneous for all fly organs and the pupa needs to be examined ventrally, dorsally and laterally to ensure accurate classification by an image analysis algorithm. When the pupae are maturing at a constant temperature of 24 °C and sorted at the appropriate age, 24 days post-larviposition for Glossina palpalis gambiensis, the sorting machine can efficiently separate the sexes. The recovered male pupae can then be sterilised for field releases of males, while the rest of the pupae can be used to maintain the laboratory colony. The sorting process with the new NIRPSS had no negative impact on adult emergence and flight ability. A mean male recovery of 62.82 ± 3.61% was enough to provide sterile males to an operational SIT programme, while mean contamination with females (4.69 ± 3.02%) was low enough to have no impact on the maintenance of a laboratory colony.
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spelling pubmed-101911112023-05-18 Near-infrared imaging for automated tsetse pupae sex sorting in support of the sterile insect technique Argilés-Herrero, Rafael Salvador-Herranz, Gustavo Parker, Andrew G. Zacarés, Mario Fall, Assane G. Gaye, Adji M. Nawaz, Arooj Takáč, Peter Vreysen, Marc J.B. de Beer, Chantel J. Parasite Research Article Tsetse flies are the cyclical vectors of African trypanosomes and one of several methods to manage this vector is the sterile insect technique (SIT). The ability to determine the sex of tsetse pupae with the objective to separate the sexes before adult emergence has been a major goal for decades for tsetse management programmes with an SIT component. Tsetse females develop faster and pharate females inside the pupae melanise 1–2 days before males. This earlier melanisation can be detected by infrared cameras through the pupal shell, and the newly developed Near InfraRed Pupae Sex Sorter (NIRPSS) takes advantage of this. The melanisation process is not homogeneous for all fly organs and the pupa needs to be examined ventrally, dorsally and laterally to ensure accurate classification by an image analysis algorithm. When the pupae are maturing at a constant temperature of 24 °C and sorted at the appropriate age, 24 days post-larviposition for Glossina palpalis gambiensis, the sorting machine can efficiently separate the sexes. The recovered male pupae can then be sterilised for field releases of males, while the rest of the pupae can be used to maintain the laboratory colony. The sorting process with the new NIRPSS had no negative impact on adult emergence and flight ability. A mean male recovery of 62.82 ± 3.61% was enough to provide sterile males to an operational SIT programme, while mean contamination with females (4.69 ± 3.02%) was low enough to have no impact on the maintenance of a laboratory colony. EDP Sciences 2023-05-17 /pmc/articles/PMC10191111/ /pubmed/37195141 http://dx.doi.org/10.1051/parasite/2023019 Text en © R. Argilés-Herrero et al., published by EDP Sciences, 2023 https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0 (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Argilés-Herrero, Rafael
Salvador-Herranz, Gustavo
Parker, Andrew G.
Zacarés, Mario
Fall, Assane G.
Gaye, Adji M.
Nawaz, Arooj
Takáč, Peter
Vreysen, Marc J.B.
de Beer, Chantel J.
Near-infrared imaging for automated tsetse pupae sex sorting in support of the sterile insect technique
title Near-infrared imaging for automated tsetse pupae sex sorting in support of the sterile insect technique
title_full Near-infrared imaging for automated tsetse pupae sex sorting in support of the sterile insect technique
title_fullStr Near-infrared imaging for automated tsetse pupae sex sorting in support of the sterile insect technique
title_full_unstemmed Near-infrared imaging for automated tsetse pupae sex sorting in support of the sterile insect technique
title_short Near-infrared imaging for automated tsetse pupae sex sorting in support of the sterile insect technique
title_sort near-infrared imaging for automated tsetse pupae sex sorting in support of the sterile insect technique
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10191111/
https://www.ncbi.nlm.nih.gov/pubmed/37195141
http://dx.doi.org/10.1051/parasite/2023019
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