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XRN2 suppresses aberrant entry of tRNA trailers into argonaute in humans and Arabidopsis
MicroRNAs (miRNAs) are a well-characterized class of small RNAs (sRNAs) that regulate gene expression post-transcriptionally. miRNAs function within a complex milieu of other sRNAs of similar size and abundance, with the best characterized being tRNA fragments or tRFs. The mechanism by which the RNA...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10191329/ https://www.ncbi.nlm.nih.gov/pubmed/37146074 http://dx.doi.org/10.1371/journal.pgen.1010755 |
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author | Wilson, Briana Su, Zhangli Kumar, Pankaj Dutta, Anindya |
author_facet | Wilson, Briana Su, Zhangli Kumar, Pankaj Dutta, Anindya |
author_sort | Wilson, Briana |
collection | PubMed |
description | MicroRNAs (miRNAs) are a well-characterized class of small RNAs (sRNAs) that regulate gene expression post-transcriptionally. miRNAs function within a complex milieu of other sRNAs of similar size and abundance, with the best characterized being tRNA fragments or tRFs. The mechanism by which the RNA-induced silencing complex (RISC) selects for specific sRNAs over others is not entirely understood in human cells. Several highly expressed tRNA trailers (tRF-1s) are strikingly similar to microRNAs in length but are generally excluded from the microRNA effector pathway. This exclusion provides a paradigm for identifying mechanisms of RISC selectivity. Here, we show that 5′ to 3′ exoribonuclease XRN2 contributes to human RISC selectivity. Although highly abundant, tRF-1s are highly unstable and degraded by XRN2 which blocks tRF-1 accumulation in RISC. We also find that XRN mediated degradation of tRF-1s and subsequent exclusion from RISC is conserved in plants. Our findings reveal a conserved mechanism that prevents aberrant entry of a class of highly produced sRNAs into Ago2. |
format | Online Article Text |
id | pubmed-10191329 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-101913292023-05-18 XRN2 suppresses aberrant entry of tRNA trailers into argonaute in humans and Arabidopsis Wilson, Briana Su, Zhangli Kumar, Pankaj Dutta, Anindya PLoS Genet Research Article MicroRNAs (miRNAs) are a well-characterized class of small RNAs (sRNAs) that regulate gene expression post-transcriptionally. miRNAs function within a complex milieu of other sRNAs of similar size and abundance, with the best characterized being tRNA fragments or tRFs. The mechanism by which the RNA-induced silencing complex (RISC) selects for specific sRNAs over others is not entirely understood in human cells. Several highly expressed tRNA trailers (tRF-1s) are strikingly similar to microRNAs in length but are generally excluded from the microRNA effector pathway. This exclusion provides a paradigm for identifying mechanisms of RISC selectivity. Here, we show that 5′ to 3′ exoribonuclease XRN2 contributes to human RISC selectivity. Although highly abundant, tRF-1s are highly unstable and degraded by XRN2 which blocks tRF-1 accumulation in RISC. We also find that XRN mediated degradation of tRF-1s and subsequent exclusion from RISC is conserved in plants. Our findings reveal a conserved mechanism that prevents aberrant entry of a class of highly produced sRNAs into Ago2. Public Library of Science 2023-05-05 /pmc/articles/PMC10191329/ /pubmed/37146074 http://dx.doi.org/10.1371/journal.pgen.1010755 Text en © 2023 Wilson et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Wilson, Briana Su, Zhangli Kumar, Pankaj Dutta, Anindya XRN2 suppresses aberrant entry of tRNA trailers into argonaute in humans and Arabidopsis |
title | XRN2 suppresses aberrant entry of tRNA trailers into argonaute in humans and Arabidopsis |
title_full | XRN2 suppresses aberrant entry of tRNA trailers into argonaute in humans and Arabidopsis |
title_fullStr | XRN2 suppresses aberrant entry of tRNA trailers into argonaute in humans and Arabidopsis |
title_full_unstemmed | XRN2 suppresses aberrant entry of tRNA trailers into argonaute in humans and Arabidopsis |
title_short | XRN2 suppresses aberrant entry of tRNA trailers into argonaute in humans and Arabidopsis |
title_sort | xrn2 suppresses aberrant entry of trna trailers into argonaute in humans and arabidopsis |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10191329/ https://www.ncbi.nlm.nih.gov/pubmed/37146074 http://dx.doi.org/10.1371/journal.pgen.1010755 |
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