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Mechanism of assembly of type 4 filaments: everything you always wanted to know (but were afraid to ask)
Type 4 filaments (T4F) are a superfamily of filamentous nanomachines – virtually ubiquitous in prokaryotes and functionally versatile – of which type 4 pili (T4P) are the defining member. T4F are polymers of type 4 pilins, assembled by conserved multi-protein machineries. They have long been an impo...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Microbiology Society
2023
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10191384/ https://www.ncbi.nlm.nih.gov/pubmed/36947586 http://dx.doi.org/10.1099/mic.0.001311 |
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author | Pelicic, Vladimir |
author_facet | Pelicic, Vladimir |
author_sort | Pelicic, Vladimir |
collection | PubMed |
description | Type 4 filaments (T4F) are a superfamily of filamentous nanomachines – virtually ubiquitous in prokaryotes and functionally versatile – of which type 4 pili (T4P) are the defining member. T4F are polymers of type 4 pilins, assembled by conserved multi-protein machineries. They have long been an important topic for research because they are key virulence factors in numerous bacterial pathogens. Our poor understanding of the molecular mechanisms of T4F assembly is a serious hindrance to the design of anti-T4F therapeutics. This review attempts to shed light on the fundamental mechanistic principles at play in T4F assembly by focusing on similarities rather than differences between several (mostly bacterial) T4F. This holistic approach, complemented by the revolutionary ability of artificial intelligence to predict protein structures, led to an intriguing mechanistic model of T4F assembly. |
format | Online Article Text |
id | pubmed-10191384 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Microbiology Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-101913842023-05-18 Mechanism of assembly of type 4 filaments: everything you always wanted to know (but were afraid to ask) Pelicic, Vladimir Microbiology (Reading) Microbial Cell Surfaces Type 4 filaments (T4F) are a superfamily of filamentous nanomachines – virtually ubiquitous in prokaryotes and functionally versatile – of which type 4 pili (T4P) are the defining member. T4F are polymers of type 4 pilins, assembled by conserved multi-protein machineries. They have long been an important topic for research because they are key virulence factors in numerous bacterial pathogens. Our poor understanding of the molecular mechanisms of T4F assembly is a serious hindrance to the design of anti-T4F therapeutics. This review attempts to shed light on the fundamental mechanistic principles at play in T4F assembly by focusing on similarities rather than differences between several (mostly bacterial) T4F. This holistic approach, complemented by the revolutionary ability of artificial intelligence to predict protein structures, led to an intriguing mechanistic model of T4F assembly. Microbiology Society 2023-03-22 /pmc/articles/PMC10191384/ /pubmed/36947586 http://dx.doi.org/10.1099/mic.0.001311 Text en © 2023 The Authors https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License. |
spellingShingle | Microbial Cell Surfaces Pelicic, Vladimir Mechanism of assembly of type 4 filaments: everything you always wanted to know (but were afraid to ask) |
title | Mechanism of assembly of type 4 filaments: everything you always wanted to know (but were afraid to ask) |
title_full | Mechanism of assembly of type 4 filaments: everything you always wanted to know (but were afraid to ask) |
title_fullStr | Mechanism of assembly of type 4 filaments: everything you always wanted to know (but were afraid to ask) |
title_full_unstemmed | Mechanism of assembly of type 4 filaments: everything you always wanted to know (but were afraid to ask) |
title_short | Mechanism of assembly of type 4 filaments: everything you always wanted to know (but were afraid to ask) |
title_sort | mechanism of assembly of type 4 filaments: everything you always wanted to know (but were afraid to ask) |
topic | Microbial Cell Surfaces |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10191384/ https://www.ncbi.nlm.nih.gov/pubmed/36947586 http://dx.doi.org/10.1099/mic.0.001311 |
work_keys_str_mv | AT pelicicvladimir mechanismofassemblyoftype4filamentseverythingyoualwayswantedtoknowbutwereafraidtoask |