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Circulating miR-146b and miR-27b are efficient biomarkers for early diagnosis of Equidae osteoarthritis
One of the most orthopedic problems seen in the equine is osteoarthritis (OA). The present study tracks some biochemical, epigenetic, and transcriptomic factors along different stages of monoiodoacetate (MIA) induced OA in donkeys in serum and synovial fluid. The aim of the study was the detection o...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Nature Publishing Group UK
2023
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10192321/ https://www.ncbi.nlm.nih.gov/pubmed/37198318 http://dx.doi.org/10.1038/s41598-023-35207-3 |
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author | Yassin, Aya M. AbuBakr, Huda O. Abdelgalil, Ahmed I. Farid, Omar A. El-Behairy, Adel M. Gouda, Eman M. |
author_facet | Yassin, Aya M. AbuBakr, Huda O. Abdelgalil, Ahmed I. Farid, Omar A. El-Behairy, Adel M. Gouda, Eman M. |
author_sort | Yassin, Aya M. |
collection | PubMed |
description | One of the most orthopedic problems seen in the equine is osteoarthritis (OA). The present study tracks some biochemical, epigenetic, and transcriptomic factors along different stages of monoiodoacetate (MIA) induced OA in donkeys in serum and synovial fluid. The aim of the study was the detection of sensitive noninvasive early biomarkers. OA was induced by a single intra-articular injection of 25 mg of MIA into the left radiocarpal joint of nine donkeys. Serum and synovial samples were taken at zero-day and different intervals for assessment of total GAGs and CS levels as well as miR-146b, miR-27b, TRAF-6, and COL10A1 gene expression. The results showed that the total GAGs and CS levels increased in different stages of OA. The level of expression of both miR-146b and miR-27b were upregulated as OA progressed and then downregulated at late stages. TRAF-6 gene was upregulated at the late stage while synovial fluid COL10A1 was over-expressed at the early stage of OA and then decreased at the late stages (P < 0.05). In conclusion, both miR-146b and miR-27b together with COL10A1 could be used as promising noninvasive biomarkers for the very early diagnosis of OA. |
format | Online Article Text |
id | pubmed-10192321 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-101923212023-05-19 Circulating miR-146b and miR-27b are efficient biomarkers for early diagnosis of Equidae osteoarthritis Yassin, Aya M. AbuBakr, Huda O. Abdelgalil, Ahmed I. Farid, Omar A. El-Behairy, Adel M. Gouda, Eman M. Sci Rep Article One of the most orthopedic problems seen in the equine is osteoarthritis (OA). The present study tracks some biochemical, epigenetic, and transcriptomic factors along different stages of monoiodoacetate (MIA) induced OA in donkeys in serum and synovial fluid. The aim of the study was the detection of sensitive noninvasive early biomarkers. OA was induced by a single intra-articular injection of 25 mg of MIA into the left radiocarpal joint of nine donkeys. Serum and synovial samples were taken at zero-day and different intervals for assessment of total GAGs and CS levels as well as miR-146b, miR-27b, TRAF-6, and COL10A1 gene expression. The results showed that the total GAGs and CS levels increased in different stages of OA. The level of expression of both miR-146b and miR-27b were upregulated as OA progressed and then downregulated at late stages. TRAF-6 gene was upregulated at the late stage while synovial fluid COL10A1 was over-expressed at the early stage of OA and then decreased at the late stages (P < 0.05). In conclusion, both miR-146b and miR-27b together with COL10A1 could be used as promising noninvasive biomarkers for the very early diagnosis of OA. Nature Publishing Group UK 2023-05-17 /pmc/articles/PMC10192321/ /pubmed/37198318 http://dx.doi.org/10.1038/s41598-023-35207-3 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Yassin, Aya M. AbuBakr, Huda O. Abdelgalil, Ahmed I. Farid, Omar A. El-Behairy, Adel M. Gouda, Eman M. Circulating miR-146b and miR-27b are efficient biomarkers for early diagnosis of Equidae osteoarthritis |
title | Circulating miR-146b and miR-27b are efficient biomarkers for early diagnosis of Equidae osteoarthritis |
title_full | Circulating miR-146b and miR-27b are efficient biomarkers for early diagnosis of Equidae osteoarthritis |
title_fullStr | Circulating miR-146b and miR-27b are efficient biomarkers for early diagnosis of Equidae osteoarthritis |
title_full_unstemmed | Circulating miR-146b and miR-27b are efficient biomarkers for early diagnosis of Equidae osteoarthritis |
title_short | Circulating miR-146b and miR-27b are efficient biomarkers for early diagnosis of Equidae osteoarthritis |
title_sort | circulating mir-146b and mir-27b are efficient biomarkers for early diagnosis of equidae osteoarthritis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10192321/ https://www.ncbi.nlm.nih.gov/pubmed/37198318 http://dx.doi.org/10.1038/s41598-023-35207-3 |
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