Lack of involvement of CD63 and CD9 tetraspanins in the extracellular vesicle content delivery process

Extracellular vesicles (EVs) are thought to mediate intercellular communication by transferring cargoes from donor to acceptor cells. The EV content-delivery process within acceptor cells is still poorly characterized and debated. CD63 and CD9, members of the tetraspanin family, are highly enriched...

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Autores principales: Tognoli, Maria Laura, Dancourt, Julia, Bonsergent, Emeline, Palmulli, Roberta, de Jong, Olivier G., Van Niel, Guillaume, Rubinstein, Eric, Vader, Pieter, Lavieu, Gregory
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10192366/
https://www.ncbi.nlm.nih.gov/pubmed/37198427
http://dx.doi.org/10.1038/s42003-023-04911-1
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author Tognoli, Maria Laura
Dancourt, Julia
Bonsergent, Emeline
Palmulli, Roberta
de Jong, Olivier G.
Van Niel, Guillaume
Rubinstein, Eric
Vader, Pieter
Lavieu, Gregory
author_facet Tognoli, Maria Laura
Dancourt, Julia
Bonsergent, Emeline
Palmulli, Roberta
de Jong, Olivier G.
Van Niel, Guillaume
Rubinstein, Eric
Vader, Pieter
Lavieu, Gregory
author_sort Tognoli, Maria Laura
collection PubMed
description Extracellular vesicles (EVs) are thought to mediate intercellular communication by transferring cargoes from donor to acceptor cells. The EV content-delivery process within acceptor cells is still poorly characterized and debated. CD63 and CD9, members of the tetraspanin family, are highly enriched within EV membranes and are respectively enriched within multivesicular bodies/endosomes and at the plasma membrane of the cells. CD63 and CD9 have been suspected to regulate the EV uptake and delivery process. Here we used two independent assays and different cell models (HeLa, MDA-MB-231 and HEK293T cells) to assess the putative role of CD63 and CD9 in the EV delivery process that includes uptake and cargo delivery. Our results suggest that neither CD63, nor CD9 are required for this function.
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spelling pubmed-101923662023-05-19 Lack of involvement of CD63 and CD9 tetraspanins in the extracellular vesicle content delivery process Tognoli, Maria Laura Dancourt, Julia Bonsergent, Emeline Palmulli, Roberta de Jong, Olivier G. Van Niel, Guillaume Rubinstein, Eric Vader, Pieter Lavieu, Gregory Commun Biol Article Extracellular vesicles (EVs) are thought to mediate intercellular communication by transferring cargoes from donor to acceptor cells. The EV content-delivery process within acceptor cells is still poorly characterized and debated. CD63 and CD9, members of the tetraspanin family, are highly enriched within EV membranes and are respectively enriched within multivesicular bodies/endosomes and at the plasma membrane of the cells. CD63 and CD9 have been suspected to regulate the EV uptake and delivery process. Here we used two independent assays and different cell models (HeLa, MDA-MB-231 and HEK293T cells) to assess the putative role of CD63 and CD9 in the EV delivery process that includes uptake and cargo delivery. Our results suggest that neither CD63, nor CD9 are required for this function. Nature Publishing Group UK 2023-05-17 /pmc/articles/PMC10192366/ /pubmed/37198427 http://dx.doi.org/10.1038/s42003-023-04911-1 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Tognoli, Maria Laura
Dancourt, Julia
Bonsergent, Emeline
Palmulli, Roberta
de Jong, Olivier G.
Van Niel, Guillaume
Rubinstein, Eric
Vader, Pieter
Lavieu, Gregory
Lack of involvement of CD63 and CD9 tetraspanins in the extracellular vesicle content delivery process
title Lack of involvement of CD63 and CD9 tetraspanins in the extracellular vesicle content delivery process
title_full Lack of involvement of CD63 and CD9 tetraspanins in the extracellular vesicle content delivery process
title_fullStr Lack of involvement of CD63 and CD9 tetraspanins in the extracellular vesicle content delivery process
title_full_unstemmed Lack of involvement of CD63 and CD9 tetraspanins in the extracellular vesicle content delivery process
title_short Lack of involvement of CD63 and CD9 tetraspanins in the extracellular vesicle content delivery process
title_sort lack of involvement of cd63 and cd9 tetraspanins in the extracellular vesicle content delivery process
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10192366/
https://www.ncbi.nlm.nih.gov/pubmed/37198427
http://dx.doi.org/10.1038/s42003-023-04911-1
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