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Antimicrobial activity of Limosilactobacillus fermentum strains isolated from the human oral cavity against Streptococcus mutans
Oral probiotics have been recently gaining much attention owing to their potential to inhibit the progression of dental caries by controlling the cariogenic effects of Streptococcus mutans. We isolated and genotypically identified 77 lactic acid bacteria including 12 Limosilactobacillus fermentum pr...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10192434/ https://www.ncbi.nlm.nih.gov/pubmed/37198248 http://dx.doi.org/10.1038/s41598-023-35168-7 |
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author | Park, Do-Young Hwang, Jiyoung Kim, Yunji Lee, Dahye Kim, Young-Youn Kim, Hye-Sung Hwang, Inseong |
author_facet | Park, Do-Young Hwang, Jiyoung Kim, Yunji Lee, Dahye Kim, Young-Youn Kim, Hye-Sung Hwang, Inseong |
author_sort | Park, Do-Young |
collection | PubMed |
description | Oral probiotics have been recently gaining much attention owing to their potential to inhibit the progression of dental caries by controlling the cariogenic effects of Streptococcus mutans. We isolated and genotypically identified 77 lactic acid bacteria including 12 Limosilactobacillus fermentum probiotic candidates from the oral cavity of healthy volunteers. Among the 12 L. fermentum isolates, nine isolates effectively inhibited the growth of S. mutans via hydrogen peroxide (H(2)O(2)) production. The others neither suppressed the growth of S. mutans nor produced H(2)O(2). Eight out of the nine H(2)O(2)-producing L. fermentum isolates exhibited strong adherence to oral epithelial KB cells while inhibiting the adherence of S. mutans to KB cells. The eight H(2)O(2)-producing isolates were neither haemolytic based on a blood-agar test, cytotoxic according to lactate dehydrogenase assay, nor resistant to eight antibiotics represented by the European Food Safety Authority guideline, indicating that the isolates have potential to suppress the cariogenesis driven by S. mutans while providing general probiotic benefits. |
format | Online Article Text |
id | pubmed-10192434 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-101924342023-05-19 Antimicrobial activity of Limosilactobacillus fermentum strains isolated from the human oral cavity against Streptococcus mutans Park, Do-Young Hwang, Jiyoung Kim, Yunji Lee, Dahye Kim, Young-Youn Kim, Hye-Sung Hwang, Inseong Sci Rep Article Oral probiotics have been recently gaining much attention owing to their potential to inhibit the progression of dental caries by controlling the cariogenic effects of Streptococcus mutans. We isolated and genotypically identified 77 lactic acid bacteria including 12 Limosilactobacillus fermentum probiotic candidates from the oral cavity of healthy volunteers. Among the 12 L. fermentum isolates, nine isolates effectively inhibited the growth of S. mutans via hydrogen peroxide (H(2)O(2)) production. The others neither suppressed the growth of S. mutans nor produced H(2)O(2). Eight out of the nine H(2)O(2)-producing L. fermentum isolates exhibited strong adherence to oral epithelial KB cells while inhibiting the adherence of S. mutans to KB cells. The eight H(2)O(2)-producing isolates were neither haemolytic based on a blood-agar test, cytotoxic according to lactate dehydrogenase assay, nor resistant to eight antibiotics represented by the European Food Safety Authority guideline, indicating that the isolates have potential to suppress the cariogenesis driven by S. mutans while providing general probiotic benefits. Nature Publishing Group UK 2023-05-17 /pmc/articles/PMC10192434/ /pubmed/37198248 http://dx.doi.org/10.1038/s41598-023-35168-7 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Park, Do-Young Hwang, Jiyoung Kim, Yunji Lee, Dahye Kim, Young-Youn Kim, Hye-Sung Hwang, Inseong Antimicrobial activity of Limosilactobacillus fermentum strains isolated from the human oral cavity against Streptococcus mutans |
title | Antimicrobial activity of Limosilactobacillus fermentum strains isolated from the human oral cavity against Streptococcus mutans |
title_full | Antimicrobial activity of Limosilactobacillus fermentum strains isolated from the human oral cavity against Streptococcus mutans |
title_fullStr | Antimicrobial activity of Limosilactobacillus fermentum strains isolated from the human oral cavity against Streptococcus mutans |
title_full_unstemmed | Antimicrobial activity of Limosilactobacillus fermentum strains isolated from the human oral cavity against Streptococcus mutans |
title_short | Antimicrobial activity of Limosilactobacillus fermentum strains isolated from the human oral cavity against Streptococcus mutans |
title_sort | antimicrobial activity of limosilactobacillus fermentum strains isolated from the human oral cavity against streptococcus mutans |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10192434/ https://www.ncbi.nlm.nih.gov/pubmed/37198248 http://dx.doi.org/10.1038/s41598-023-35168-7 |
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