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The efficacy of therapeutic plasma exchange in COVID-19 patients on endothelial tightness in vitro is hindered by platelet activation

Coronavirus disease (COVID)-19 is characterised in particular by vascular inflammation with platelet activation and endothelial dysfunction. During the pandemic, therapeutic plasma exchange (TPE) was used to reduce the cytokine storm in the circulation and delay or prevent ICU admissions. This proce...

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Autores principales: Ebermeyer, Theo, Hequet, Olivier, Berard, Frederic, Prier, Amelie, Eyraud, Marie-Ange, Arthaud, Charles-Antoine, Heestermans, Marco, Duchez, Anne-Claire, Guironnet-Paquet, Aurelie, Berthelot, Philippe, Cognasse, Fabrice, Hamzeh-Cognasse, Hind
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10192624/
https://www.ncbi.nlm.nih.gov/pubmed/37215546
http://dx.doi.org/10.3389/fcvm.2023.1094786
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author Ebermeyer, Theo
Hequet, Olivier
Berard, Frederic
Prier, Amelie
Eyraud, Marie-Ange
Arthaud, Charles-Antoine
Heestermans, Marco
Duchez, Anne-Claire
Guironnet-Paquet, Aurelie
Berthelot, Philippe
Cognasse, Fabrice
Hamzeh-Cognasse, Hind
author_facet Ebermeyer, Theo
Hequet, Olivier
Berard, Frederic
Prier, Amelie
Eyraud, Marie-Ange
Arthaud, Charles-Antoine
Heestermans, Marco
Duchez, Anne-Claire
Guironnet-Paquet, Aurelie
Berthelot, Philippe
Cognasse, Fabrice
Hamzeh-Cognasse, Hind
author_sort Ebermeyer, Theo
collection PubMed
description Coronavirus disease (COVID)-19 is characterised in particular by vascular inflammation with platelet activation and endothelial dysfunction. During the pandemic, therapeutic plasma exchange (TPE) was used to reduce the cytokine storm in the circulation and delay or prevent ICU admissions. This procedure consists in replacing the inflammatory plasma by fresh frozen plasma from healthy donors and is often used to remove pathogenic molecules from plasma (autoantibodies, immune complexes, toxins, etc.). This study uses an in vitro model of platelet-endothelial cell interactions to assess changes in these interactions by plasma from COVID-19 patients and to determine the extent to which TPE reduces such changes. We noted that exposure of an endothelial monolayer to plasmas from COVID-19 patients post-TPE induced less endothelial permeability compared to COVID-19 control plasmas. Yet, when endothelial cells were co-cultured with healthy platelets and exposed to the plasma, the beneficial effect of TPE on endothelial permeability was somewhat reduced. This was linked to platelet and endothelial phenotypical activation but not with inflammatory molecule secretion. Our work shows that, in parallel to the beneficial removal of inflammatory factors from the circulation, TPE triggers cellular activation which may partly explain the reduction in efficacy in terms of endothelial dysfunction. These findings provide new insights for improving the efficacy of TPE using supporting treatments targeting platelet activation, for instance.
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spelling pubmed-101926242023-05-19 The efficacy of therapeutic plasma exchange in COVID-19 patients on endothelial tightness in vitro is hindered by platelet activation Ebermeyer, Theo Hequet, Olivier Berard, Frederic Prier, Amelie Eyraud, Marie-Ange Arthaud, Charles-Antoine Heestermans, Marco Duchez, Anne-Claire Guironnet-Paquet, Aurelie Berthelot, Philippe Cognasse, Fabrice Hamzeh-Cognasse, Hind Front Cardiovasc Med Cardiovascular Medicine Coronavirus disease (COVID)-19 is characterised in particular by vascular inflammation with platelet activation and endothelial dysfunction. During the pandemic, therapeutic plasma exchange (TPE) was used to reduce the cytokine storm in the circulation and delay or prevent ICU admissions. This procedure consists in replacing the inflammatory plasma by fresh frozen plasma from healthy donors and is often used to remove pathogenic molecules from plasma (autoantibodies, immune complexes, toxins, etc.). This study uses an in vitro model of platelet-endothelial cell interactions to assess changes in these interactions by plasma from COVID-19 patients and to determine the extent to which TPE reduces such changes. We noted that exposure of an endothelial monolayer to plasmas from COVID-19 patients post-TPE induced less endothelial permeability compared to COVID-19 control plasmas. Yet, when endothelial cells were co-cultured with healthy platelets and exposed to the plasma, the beneficial effect of TPE on endothelial permeability was somewhat reduced. This was linked to platelet and endothelial phenotypical activation but not with inflammatory molecule secretion. Our work shows that, in parallel to the beneficial removal of inflammatory factors from the circulation, TPE triggers cellular activation which may partly explain the reduction in efficacy in terms of endothelial dysfunction. These findings provide new insights for improving the efficacy of TPE using supporting treatments targeting platelet activation, for instance. Frontiers Media S.A. 2023-05-04 /pmc/articles/PMC10192624/ /pubmed/37215546 http://dx.doi.org/10.3389/fcvm.2023.1094786 Text en © 2023 Ebermeyer, Hequet, Berard, Prier, Eyraud, Arthaud, Heestermans, Duchez, Guironnet- Paquet, Berthelot, Cognasse and Hamzeh-Cognasse. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY) (https://creativecommons.org/licenses/by/4.0/) . The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cardiovascular Medicine
Ebermeyer, Theo
Hequet, Olivier
Berard, Frederic
Prier, Amelie
Eyraud, Marie-Ange
Arthaud, Charles-Antoine
Heestermans, Marco
Duchez, Anne-Claire
Guironnet-Paquet, Aurelie
Berthelot, Philippe
Cognasse, Fabrice
Hamzeh-Cognasse, Hind
The efficacy of therapeutic plasma exchange in COVID-19 patients on endothelial tightness in vitro is hindered by platelet activation
title The efficacy of therapeutic plasma exchange in COVID-19 patients on endothelial tightness in vitro is hindered by platelet activation
title_full The efficacy of therapeutic plasma exchange in COVID-19 patients on endothelial tightness in vitro is hindered by platelet activation
title_fullStr The efficacy of therapeutic plasma exchange in COVID-19 patients on endothelial tightness in vitro is hindered by platelet activation
title_full_unstemmed The efficacy of therapeutic plasma exchange in COVID-19 patients on endothelial tightness in vitro is hindered by platelet activation
title_short The efficacy of therapeutic plasma exchange in COVID-19 patients on endothelial tightness in vitro is hindered by platelet activation
title_sort efficacy of therapeutic plasma exchange in covid-19 patients on endothelial tightness in vitro is hindered by platelet activation
topic Cardiovascular Medicine
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10192624/
https://www.ncbi.nlm.nih.gov/pubmed/37215546
http://dx.doi.org/10.3389/fcvm.2023.1094786
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