Whole-mount immunofluorescence staining of blood and lymphatic vessels in murine small intestine

The small intestine is an excellent model for studying changes in vasculature in response to different diseases or gene deletions. Here, we present a protocol for whole-mount immunofluorescence staining of blood and lymphatic vessels in the adult mouse small intestine. We describe the steps for perf...

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Detalles Bibliográficos
Autores principales: Paavonsalo, Satu, Subashi, Yelin, Lackman, Madeleine H., Karaman, Sinem
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10192636/
https://www.ncbi.nlm.nih.gov/pubmed/37182207
http://dx.doi.org/10.1016/j.xpro.2023.102310
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author Paavonsalo, Satu
Subashi, Yelin
Lackman, Madeleine H.
Karaman, Sinem
author_facet Paavonsalo, Satu
Subashi, Yelin
Lackman, Madeleine H.
Karaman, Sinem
author_sort Paavonsalo, Satu
collection PubMed
description The small intestine is an excellent model for studying changes in vasculature in response to different diseases or gene deletions. Here, we present a protocol for whole-mount immunofluorescence staining of blood and lymphatic vessels in the adult mouse small intestine. We describe the steps for perfusion fixation, tissue sample preparation, immunofluorescence staining, and whole-mount preparation of stained samples. Our protocol will enable researchers to visualize and analyze the intricate network of vessels in the small intestine. For complete details on the use and execution of this protocol, please refer to Karaman et al. (2022).(1)
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spelling pubmed-101926362023-05-19 Whole-mount immunofluorescence staining of blood and lymphatic vessels in murine small intestine Paavonsalo, Satu Subashi, Yelin Lackman, Madeleine H. Karaman, Sinem STAR Protoc Protocol The small intestine is an excellent model for studying changes in vasculature in response to different diseases or gene deletions. Here, we present a protocol for whole-mount immunofluorescence staining of blood and lymphatic vessels in the adult mouse small intestine. We describe the steps for perfusion fixation, tissue sample preparation, immunofluorescence staining, and whole-mount preparation of stained samples. Our protocol will enable researchers to visualize and analyze the intricate network of vessels in the small intestine. For complete details on the use and execution of this protocol, please refer to Karaman et al. (2022).(1) Elsevier 2023-05-13 /pmc/articles/PMC10192636/ /pubmed/37182207 http://dx.doi.org/10.1016/j.xpro.2023.102310 Text en © 2023 The Author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Protocol
Paavonsalo, Satu
Subashi, Yelin
Lackman, Madeleine H.
Karaman, Sinem
Whole-mount immunofluorescence staining of blood and lymphatic vessels in murine small intestine
title Whole-mount immunofluorescence staining of blood and lymphatic vessels in murine small intestine
title_full Whole-mount immunofluorescence staining of blood and lymphatic vessels in murine small intestine
title_fullStr Whole-mount immunofluorescence staining of blood and lymphatic vessels in murine small intestine
title_full_unstemmed Whole-mount immunofluorescence staining of blood and lymphatic vessels in murine small intestine
title_short Whole-mount immunofluorescence staining of blood and lymphatic vessels in murine small intestine
title_sort whole-mount immunofluorescence staining of blood and lymphatic vessels in murine small intestine
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10192636/
https://www.ncbi.nlm.nih.gov/pubmed/37182207
http://dx.doi.org/10.1016/j.xpro.2023.102310
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AT lackmanmadeleineh wholemountimmunofluorescencestainingofbloodandlymphaticvesselsinmurinesmallintestine
AT karamansinem wholemountimmunofluorescencestainingofbloodandlymphaticvesselsinmurinesmallintestine

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