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Detection of specific RBD(+) IgG(+) memory B cells by flow cytometry in healthcare workers and patients with inborn errors of immunity after BNT162b2 m RNA COVID-19 vaccination

INTRODUCTION: Inborn errors of immunity (IEI) are a heterogeneous group of diseases caused by intrinsic defects of the immune system. Estimating the immune competence of immunocompromised patients for an infection risk assessment or after SARS-CoV-2 vaccination constituted a challenge. METHODS: The...

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Autores principales: del Pino Molina, Lucía, Bravo Gallego, Luz Yadira, Nozal, Pilar, Soto-Serrano, Yolanda, Martínez-Feito, Ana, Reche-Yebra, Keren, González-Torbay, Andrea, Cuesta-Martín de la Cámara, Ricardo, Gianelli, Carla, Cámara, Carmen, González-García, J., González-Muñoz, Miguel, Rodríguez-Pena, Rebeca, López Granados, Eduardo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10192857/
https://www.ncbi.nlm.nih.gov/pubmed/37215146
http://dx.doi.org/10.3389/fimmu.2023.1136308
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author del Pino Molina, Lucía
Bravo Gallego, Luz Yadira
Nozal, Pilar
Soto-Serrano, Yolanda
Martínez-Feito, Ana
Reche-Yebra, Keren
González-Torbay, Andrea
Cuesta-Martín de la Cámara, Ricardo
Gianelli, Carla
Cámara, Carmen
González-García, J.
González-Muñoz, Miguel
Rodríguez-Pena, Rebeca
López Granados, Eduardo
author_facet del Pino Molina, Lucía
Bravo Gallego, Luz Yadira
Nozal, Pilar
Soto-Serrano, Yolanda
Martínez-Feito, Ana
Reche-Yebra, Keren
González-Torbay, Andrea
Cuesta-Martín de la Cámara, Ricardo
Gianelli, Carla
Cámara, Carmen
González-García, J.
González-Muñoz, Miguel
Rodríguez-Pena, Rebeca
López Granados, Eduardo
author_sort del Pino Molina, Lucía
collection PubMed
description INTRODUCTION: Inborn errors of immunity (IEI) are a heterogeneous group of diseases caused by intrinsic defects of the immune system. Estimating the immune competence of immunocompromised patients for an infection risk assessment or after SARS-CoV-2 vaccination constituted a challenge. METHODS: The aim of this study was to determine the humoral responses of patients with IEI through a comprehensive analysis of specific receptor-binding domain-positive (RBD+) IgG+ memory B cells (MBCs) by flow cytometry, together with routine S-specific IgG antibodies and QuantiFERON SARS-CoV-2 (T-cell response), before the vaccine and 3 weeks after a second dose. RESULTS AND DISCUSSION: We first analyzed the percentage of specific RBD+ IgG+ MBCs in healthy healthcare workers. Within the control group, there was an increase in the percentage of specific IgG+ RBD+ MBCs 21 days after the second dose, which was consistent with S-specific IgG antibodies.Thirty-one patients with IEI were included for the pre- and post-vaccination study; IgG+ RBD+ MBCs were not evaluated in 6 patients due to an absence of B cells in peripheral blood. We detected various patterns among the patients with IEI with circulating B cells (25, 81%): an adequate humoral response was observed in 12/25, consider by the detection of positive S-specific IgG antibodies and the presence of specific IgG+ RBD+ MBCs, presenting a positive T-cell response; in 4/25, very low S-specific IgG antibody counts correlated with undetectable events in the IgG+ RBD+ MBC compartment but with positive cellular response. Despite the presence of S-specific IgG antibodies, we were unable to detect a relevant percentage of IgG+ RBD+ MBCs in 5/25; however, all presented positive T-cell response. Lastly, we observed a profound failure of B and T-cell response in 3 (10%) patients with IEI, with no assessment of S-specific IgG antibodies, IgG+ RBD+ MBCs, and negative cellular response. The identification of specific IgG+ RBD+ MBCs by flow cytometry provides information on different humoral immune response outcomes in patients with IEI and aids the assessment of immune competence status after SARS-CoV-2 mRNA vaccine (BNT162b2), together with S-specific IgG antibodies and T-cell responses.
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spelling pubmed-101928572023-05-19 Detection of specific RBD(+) IgG(+) memory B cells by flow cytometry in healthcare workers and patients with inborn errors of immunity after BNT162b2 m RNA COVID-19 vaccination del Pino Molina, Lucía Bravo Gallego, Luz Yadira Nozal, Pilar Soto-Serrano, Yolanda Martínez-Feito, Ana Reche-Yebra, Keren González-Torbay, Andrea Cuesta-Martín de la Cámara, Ricardo Gianelli, Carla Cámara, Carmen González-García, J. González-Muñoz, Miguel Rodríguez-Pena, Rebeca López Granados, Eduardo Front Immunol Immunology INTRODUCTION: Inborn errors of immunity (IEI) are a heterogeneous group of diseases caused by intrinsic defects of the immune system. Estimating the immune competence of immunocompromised patients for an infection risk assessment or after SARS-CoV-2 vaccination constituted a challenge. METHODS: The aim of this study was to determine the humoral responses of patients with IEI through a comprehensive analysis of specific receptor-binding domain-positive (RBD+) IgG+ memory B cells (MBCs) by flow cytometry, together with routine S-specific IgG antibodies and QuantiFERON SARS-CoV-2 (T-cell response), before the vaccine and 3 weeks after a second dose. RESULTS AND DISCUSSION: We first analyzed the percentage of specific RBD+ IgG+ MBCs in healthy healthcare workers. Within the control group, there was an increase in the percentage of specific IgG+ RBD+ MBCs 21 days after the second dose, which was consistent with S-specific IgG antibodies.Thirty-one patients with IEI were included for the pre- and post-vaccination study; IgG+ RBD+ MBCs were not evaluated in 6 patients due to an absence of B cells in peripheral blood. We detected various patterns among the patients with IEI with circulating B cells (25, 81%): an adequate humoral response was observed in 12/25, consider by the detection of positive S-specific IgG antibodies and the presence of specific IgG+ RBD+ MBCs, presenting a positive T-cell response; in 4/25, very low S-specific IgG antibody counts correlated with undetectable events in the IgG+ RBD+ MBC compartment but with positive cellular response. Despite the presence of S-specific IgG antibodies, we were unable to detect a relevant percentage of IgG+ RBD+ MBCs in 5/25; however, all presented positive T-cell response. Lastly, we observed a profound failure of B and T-cell response in 3 (10%) patients with IEI, with no assessment of S-specific IgG antibodies, IgG+ RBD+ MBCs, and negative cellular response. The identification of specific IgG+ RBD+ MBCs by flow cytometry provides information on different humoral immune response outcomes in patients with IEI and aids the assessment of immune competence status after SARS-CoV-2 mRNA vaccine (BNT162b2), together with S-specific IgG antibodies and T-cell responses. Frontiers Media S.A. 2023-05-04 /pmc/articles/PMC10192857/ /pubmed/37215146 http://dx.doi.org/10.3389/fimmu.2023.1136308 Text en Copyright © 2023 del Pino Molina, Bravo Gallego, Nozal, Soto-Serrano, Martínez-Feito, Reche-Yebra, González-Torbay, Cuesta-Martín de la Cámara, Gianelli, Cámara, González-García, González-Muñoz, Rodríguez-Pena and López Granados https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
del Pino Molina, Lucía
Bravo Gallego, Luz Yadira
Nozal, Pilar
Soto-Serrano, Yolanda
Martínez-Feito, Ana
Reche-Yebra, Keren
González-Torbay, Andrea
Cuesta-Martín de la Cámara, Ricardo
Gianelli, Carla
Cámara, Carmen
González-García, J.
González-Muñoz, Miguel
Rodríguez-Pena, Rebeca
López Granados, Eduardo
Detection of specific RBD(+) IgG(+) memory B cells by flow cytometry in healthcare workers and patients with inborn errors of immunity after BNT162b2 m RNA COVID-19 vaccination
title Detection of specific RBD(+) IgG(+) memory B cells by flow cytometry in healthcare workers and patients with inborn errors of immunity after BNT162b2 m RNA COVID-19 vaccination
title_full Detection of specific RBD(+) IgG(+) memory B cells by flow cytometry in healthcare workers and patients with inborn errors of immunity after BNT162b2 m RNA COVID-19 vaccination
title_fullStr Detection of specific RBD(+) IgG(+) memory B cells by flow cytometry in healthcare workers and patients with inborn errors of immunity after BNT162b2 m RNA COVID-19 vaccination
title_full_unstemmed Detection of specific RBD(+) IgG(+) memory B cells by flow cytometry in healthcare workers and patients with inborn errors of immunity after BNT162b2 m RNA COVID-19 vaccination
title_short Detection of specific RBD(+) IgG(+) memory B cells by flow cytometry in healthcare workers and patients with inborn errors of immunity after BNT162b2 m RNA COVID-19 vaccination
title_sort detection of specific rbd(+) igg(+) memory b cells by flow cytometry in healthcare workers and patients with inborn errors of immunity after bnt162b2 m rna covid-19 vaccination
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10192857/
https://www.ncbi.nlm.nih.gov/pubmed/37215146
http://dx.doi.org/10.3389/fimmu.2023.1136308
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