Reproducible and scalable differentiation of highly pure cortical neurons from human induced pluripotent stem cells

Human-induced-pluripotent-stem-cell (hiPSC)-derived neurons are valuable for investigating brain physiology and disease. Here, we present a protocol to differentiate hiPSCs into cortical neurons with high yield and purity. We describe neural induction via dual-SMAD inhibition, followed by spot-based...

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Detalles Bibliográficos
Autores principales: Dannert, Angelika, Klimmt, Julien, Cardoso Gonçalves, Carolina, Crusius, Dennis, Paquet, Dominik
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10193006/
https://www.ncbi.nlm.nih.gov/pubmed/37148244
http://dx.doi.org/10.1016/j.xpro.2023.102266

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