rol-6 and dpy-10 C. elegans mutants have normal mitochondrial function after normalizing to delayed development

Collagen mutations are commonly used in the creation of Caenorhabditis elegans transgenic strains, but their secondary effects are not fully characterized . We compared the mitochondrial function of N2, dpy-10, rol-6, and PE255 C. elegans . N2 worms exhibited ~2-fold greater volume, mitochondrial DN...

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Detalles Bibliográficos
Autores principales: Sparling, A. Clare, King, Dillon E., Meyer, Joel N.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Caltech Library 2023
Materias:
New Finding
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10193146/
https://www.ncbi.nlm.nih.gov/pubmed/37215639
http://dx.doi.org/10.17912/micropub.biology.000798
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author Sparling, A. Clare
King, Dillon E.
Meyer, Joel N.
author_facet Sparling, A. Clare
King, Dillon E.
Meyer, Joel N.
author_sort Sparling, A. Clare
collection PubMed
description Collagen mutations are commonly used in the creation of Caenorhabditis elegans transgenic strains, but their secondary effects are not fully characterized . We compared the mitochondrial function of N2, dpy-10, rol-6, and PE255 C. elegans . N2 worms exhibited ~2-fold greater volume, mitochondrial DNA copy number, and nuclear DNA copy number than collagen mutants (p<0.05). Whole-worm respirometry and ATP levels were higher in N2 worms, but differences in respirometry largely disappeared after normalization to mitochondrial DNA copy number. This data suggests that rol-6 and dpy-10 mutants are developmentally delayed but have comparable mitochondrial function to N2 worms once the data is normalized to developmental stage.
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spelling pubmed-101931462023-05-19 rol-6 and dpy-10 C. elegans mutants have normal mitochondrial function after normalizing to delayed development Sparling, A. Clare King, Dillon E. Meyer, Joel N. MicroPubl Biol New Finding Collagen mutations are commonly used in the creation of Caenorhabditis elegans transgenic strains, but their secondary effects are not fully characterized . We compared the mitochondrial function of N2, dpy-10, rol-6, and PE255 C. elegans . N2 worms exhibited ~2-fold greater volume, mitochondrial DNA copy number, and nuclear DNA copy number than collagen mutants (p<0.05). Whole-worm respirometry and ATP levels were higher in N2 worms, but differences in respirometry largely disappeared after normalization to mitochondrial DNA copy number. This data suggests that rol-6 and dpy-10 mutants are developmentally delayed but have comparable mitochondrial function to N2 worms once the data is normalized to developmental stage. Caltech Library 2023-05-03 /pmc/articles/PMC10193146/ /pubmed/37215639 http://dx.doi.org/10.17912/micropub.biology.000798 Text en Copyright: © 2023 by the authors https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle New Finding
Sparling, A. Clare
King, Dillon E.
Meyer, Joel N.
rol-6 and dpy-10 C. elegans mutants have normal mitochondrial function after normalizing to delayed development
title rol-6 and dpy-10 C. elegans mutants have normal mitochondrial function after normalizing to delayed development
title_full rol-6 and dpy-10 C. elegans mutants have normal mitochondrial function after normalizing to delayed development
title_fullStr rol-6 and dpy-10 C. elegans mutants have normal mitochondrial function after normalizing to delayed development
title_full_unstemmed rol-6 and dpy-10 C. elegans mutants have normal mitochondrial function after normalizing to delayed development
title_short rol-6 and dpy-10 C. elegans mutants have normal mitochondrial function after normalizing to delayed development
title_sort rol-6 and dpy-10 c. elegans mutants have normal mitochondrial function after normalizing to delayed development
topic New Finding
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10193146/
https://www.ncbi.nlm.nih.gov/pubmed/37215639
http://dx.doi.org/10.17912/micropub.biology.000798
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