Protocol for assessing the healthspan of Caenorhabditis elegans after potential anti-aging drug treatment

The nematode Caenorhabditis elegans has been developed as a valuable genetic model for research on aging and aging-related diseases. Here, we present a protocol for assessing the healthspan of C. elegans after treatment of a potential anti-aging drug. We describe steps for C. elegans synchronization...

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Detalles Bibliográficos
Autores principales: Xiao, Yi, Zhang, Li, Liu, Yun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10193290/
https://www.ncbi.nlm.nih.gov/pubmed/37148246
http://dx.doi.org/10.1016/j.xpro.2023.102285
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author Xiao, Yi
Zhang, Li
Liu, Yun
author_facet Xiao, Yi
Zhang, Li
Liu, Yun
author_sort Xiao, Yi
collection PubMed
description The nematode Caenorhabditis elegans has been developed as a valuable genetic model for research on aging and aging-related diseases. Here, we present a protocol for assessing the healthspan of C. elegans after treatment of a potential anti-aging drug. We describe steps for C. elegans synchronization, drug treatment, and lifespan determination from the survivorship curve. We also detail assessment of locomotory ability through body bend rate and measurement of lipofuscin fluorescence to quantify age pigment in the worm intestine. For complete details on the use and execution of this protocol, please refer to Xiao et al. (2022).(1)
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spelling pubmed-101932902023-05-19 Protocol for assessing the healthspan of Caenorhabditis elegans after potential anti-aging drug treatment Xiao, Yi Zhang, Li Liu, Yun STAR Protoc Protocol The nematode Caenorhabditis elegans has been developed as a valuable genetic model for research on aging and aging-related diseases. Here, we present a protocol for assessing the healthspan of C. elegans after treatment of a potential anti-aging drug. We describe steps for C. elegans synchronization, drug treatment, and lifespan determination from the survivorship curve. We also detail assessment of locomotory ability through body bend rate and measurement of lipofuscin fluorescence to quantify age pigment in the worm intestine. For complete details on the use and execution of this protocol, please refer to Xiao et al. (2022).(1) Elsevier 2023-05-05 /pmc/articles/PMC10193290/ /pubmed/37148246 http://dx.doi.org/10.1016/j.xpro.2023.102285 Text en © 2023 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Xiao, Yi
Zhang, Li
Liu, Yun
Protocol for assessing the healthspan of Caenorhabditis elegans after potential anti-aging drug treatment
title Protocol for assessing the healthspan of Caenorhabditis elegans after potential anti-aging drug treatment
title_full Protocol for assessing the healthspan of Caenorhabditis elegans after potential anti-aging drug treatment
title_fullStr Protocol for assessing the healthspan of Caenorhabditis elegans after potential anti-aging drug treatment
title_full_unstemmed Protocol for assessing the healthspan of Caenorhabditis elegans after potential anti-aging drug treatment
title_short Protocol for assessing the healthspan of Caenorhabditis elegans after potential anti-aging drug treatment
title_sort protocol for assessing the healthspan of caenorhabditis elegans after potential anti-aging drug treatment
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10193290/
https://www.ncbi.nlm.nih.gov/pubmed/37148246
http://dx.doi.org/10.1016/j.xpro.2023.102285
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