Detection of endogenous circadian rhythms of clock gene mRNA expression in mouse lung tissue using slice cultures

Detection of endogenous circadian rhythms in clock gene mRNA expression requires that mice be sacrificed at regular time intervals over one or more days. This protocol uses culture tissue slices obtained from a single mouse to collect time-course samples. We describe the procedure from preparation o...

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Detalles Bibliográficos
Autores principales: Matsumura, Ritsuko, Node, Koichi, Akashi, Makoto
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10193295/
https://www.ncbi.nlm.nih.gov/pubmed/37149856
http://dx.doi.org/10.1016/j.xpro.2023.102280

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10193295/
https://www.ncbi.nlm.nih.gov/pubmed/37149856
http://dx.doi.org/10.1016/j.xpro.2023.102280

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