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Detection of endogenous circadian rhythms of clock gene mRNA expression in mouse lung tissue using slice cultures
Detection of endogenous circadian rhythms in clock gene mRNA expression requires that mice be sacrificed at regular time intervals over one or more days. This protocol uses culture tissue slices obtained from a single mouse to collect time-course samples. We describe the procedure from preparation o...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2023
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10193295/ https://www.ncbi.nlm.nih.gov/pubmed/37149856 http://dx.doi.org/10.1016/j.xpro.2023.102280 |