Cargando…
Novel biological aqua crust enhances in situ metal(loid) bioremediation driven by phototrophic/diazotrophic biofilm
BACKGROUND: Understanding the ecological and environmental functions of phototrophic biofilms in the biological crust is crucial for improving metal(loid) (e.g. Cd, As) bioremediation in mining ecosystems. In this study, in combination with metal(loid) monitoring and metagenomic analysis, we systema...
Autores principales: | , , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10193787/ https://www.ncbi.nlm.nih.gov/pubmed/37202810 http://dx.doi.org/10.1186/s40168-023-01549-3 |
_version_ | 1785043888284631040 |
---|---|
author | Wang, Guobao Yin, Xiuran Feng, Zekai Chen, Chiyu Chen, Daijie Wu, Bo Liu, Chong Morel, Jean Louis Jiang, Yuanyuan Yu, Hang He, Huan Chao, Yuanqing Tang, Yetao Qiu, Rongliang Wang, Shizhong |
author_facet | Wang, Guobao Yin, Xiuran Feng, Zekai Chen, Chiyu Chen, Daijie Wu, Bo Liu, Chong Morel, Jean Louis Jiang, Yuanyuan Yu, Hang He, Huan Chao, Yuanqing Tang, Yetao Qiu, Rongliang Wang, Shizhong |
author_sort | Wang, Guobao |
collection | PubMed |
description | BACKGROUND: Understanding the ecological and environmental functions of phototrophic biofilms in the biological crust is crucial for improving metal(loid) (e.g. Cd, As) bioremediation in mining ecosystems. In this study, in combination with metal(loid) monitoring and metagenomic analysis, we systematically evaluated the effect of biofilm in a novel biological aqua crust (biogenic aqua crust—BAC) on in situ metal(loid) bioremediation of a representative Pb/Zn tailing pond. RESULTS: We observed strong accumulation of potentially bioavailable metal(loid)s and visible phototrophic biofilms in the BAC. Furthermore, dominating taxa Leptolyngbyaceae (10.2–10.4%, Cyanobacteria) and Cytophagales (12.3–22.1%, Bacteroidota) were enriched in biofilm. Along with predominant heterotrophs (e.g. Cytophagales sp.) as well as diazotrophs (e.g. Hyphomonadaceae sp.), autotrophs/diazotrophs (e.g. Leptolyngbyaceae sp.) in phototrophic biofilm enriched the genes encoding extracellular peptidase (e.g. family S9, S1), CAZymes (e.g. CBM50, GT2) and biofilm formation (e.g. OmpR, CRP and LuxS), thus enhancing the capacity of nutrient accumulation and metal(loid) bioremediation in BAC system. CONCLUSIONS: Our study demonstrated that a phototrophic/diazotrophic biofilm constitutes the structured communities containing specific autotrophs (e.g. Leptolyngbyaceae sp.) and heterotrophs (e.g. Cytophagales sp.), which effectively control metal(loid) and nutrient input using solar energy in aquatic environments. Elucidation of the mechanisms of biofilm formation coupled with metal(loid) immobilization in BAC expands the fundamental understanding of the geochemical fate of metal(loid)s, which may be harnessed to enhance in situ metal(loid) bioremediation in the aquatic ecosystem of the mining area. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s40168-023-01549-3. |
format | Online Article Text |
id | pubmed-10193787 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-101937872023-05-19 Novel biological aqua crust enhances in situ metal(loid) bioremediation driven by phototrophic/diazotrophic biofilm Wang, Guobao Yin, Xiuran Feng, Zekai Chen, Chiyu Chen, Daijie Wu, Bo Liu, Chong Morel, Jean Louis Jiang, Yuanyuan Yu, Hang He, Huan Chao, Yuanqing Tang, Yetao Qiu, Rongliang Wang, Shizhong Microbiome Research BACKGROUND: Understanding the ecological and environmental functions of phototrophic biofilms in the biological crust is crucial for improving metal(loid) (e.g. Cd, As) bioremediation in mining ecosystems. In this study, in combination with metal(loid) monitoring and metagenomic analysis, we systematically evaluated the effect of biofilm in a novel biological aqua crust (biogenic aqua crust—BAC) on in situ metal(loid) bioremediation of a representative Pb/Zn tailing pond. RESULTS: We observed strong accumulation of potentially bioavailable metal(loid)s and visible phototrophic biofilms in the BAC. Furthermore, dominating taxa Leptolyngbyaceae (10.2–10.4%, Cyanobacteria) and Cytophagales (12.3–22.1%, Bacteroidota) were enriched in biofilm. Along with predominant heterotrophs (e.g. Cytophagales sp.) as well as diazotrophs (e.g. Hyphomonadaceae sp.), autotrophs/diazotrophs (e.g. Leptolyngbyaceae sp.) in phototrophic biofilm enriched the genes encoding extracellular peptidase (e.g. family S9, S1), CAZymes (e.g. CBM50, GT2) and biofilm formation (e.g. OmpR, CRP and LuxS), thus enhancing the capacity of nutrient accumulation and metal(loid) bioremediation in BAC system. CONCLUSIONS: Our study demonstrated that a phototrophic/diazotrophic biofilm constitutes the structured communities containing specific autotrophs (e.g. Leptolyngbyaceae sp.) and heterotrophs (e.g. Cytophagales sp.), which effectively control metal(loid) and nutrient input using solar energy in aquatic environments. Elucidation of the mechanisms of biofilm formation coupled with metal(loid) immobilization in BAC expands the fundamental understanding of the geochemical fate of metal(loid)s, which may be harnessed to enhance in situ metal(loid) bioremediation in the aquatic ecosystem of the mining area. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s40168-023-01549-3. BioMed Central 2023-05-18 /pmc/articles/PMC10193787/ /pubmed/37202810 http://dx.doi.org/10.1186/s40168-023-01549-3 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Wang, Guobao Yin, Xiuran Feng, Zekai Chen, Chiyu Chen, Daijie Wu, Bo Liu, Chong Morel, Jean Louis Jiang, Yuanyuan Yu, Hang He, Huan Chao, Yuanqing Tang, Yetao Qiu, Rongliang Wang, Shizhong Novel biological aqua crust enhances in situ metal(loid) bioremediation driven by phototrophic/diazotrophic biofilm |
title | Novel biological aqua crust enhances in situ metal(loid) bioremediation driven by phototrophic/diazotrophic biofilm |
title_full | Novel biological aqua crust enhances in situ metal(loid) bioremediation driven by phototrophic/diazotrophic biofilm |
title_fullStr | Novel biological aqua crust enhances in situ metal(loid) bioremediation driven by phototrophic/diazotrophic biofilm |
title_full_unstemmed | Novel biological aqua crust enhances in situ metal(loid) bioremediation driven by phototrophic/diazotrophic biofilm |
title_short | Novel biological aqua crust enhances in situ metal(loid) bioremediation driven by phototrophic/diazotrophic biofilm |
title_sort | novel biological aqua crust enhances in situ metal(loid) bioremediation driven by phototrophic/diazotrophic biofilm |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10193787/ https://www.ncbi.nlm.nih.gov/pubmed/37202810 http://dx.doi.org/10.1186/s40168-023-01549-3 |
work_keys_str_mv | AT wangguobao novelbiologicalaquacrustenhancesinsitumetalloidbioremediationdrivenbyphototrophicdiazotrophicbiofilm AT yinxiuran novelbiologicalaquacrustenhancesinsitumetalloidbioremediationdrivenbyphototrophicdiazotrophicbiofilm AT fengzekai novelbiologicalaquacrustenhancesinsitumetalloidbioremediationdrivenbyphototrophicdiazotrophicbiofilm AT chenchiyu novelbiologicalaquacrustenhancesinsitumetalloidbioremediationdrivenbyphototrophicdiazotrophicbiofilm AT chendaijie novelbiologicalaquacrustenhancesinsitumetalloidbioremediationdrivenbyphototrophicdiazotrophicbiofilm AT wubo novelbiologicalaquacrustenhancesinsitumetalloidbioremediationdrivenbyphototrophicdiazotrophicbiofilm AT liuchong novelbiologicalaquacrustenhancesinsitumetalloidbioremediationdrivenbyphototrophicdiazotrophicbiofilm AT moreljeanlouis novelbiologicalaquacrustenhancesinsitumetalloidbioremediationdrivenbyphototrophicdiazotrophicbiofilm AT jiangyuanyuan novelbiologicalaquacrustenhancesinsitumetalloidbioremediationdrivenbyphototrophicdiazotrophicbiofilm AT yuhang novelbiologicalaquacrustenhancesinsitumetalloidbioremediationdrivenbyphototrophicdiazotrophicbiofilm AT hehuan novelbiologicalaquacrustenhancesinsitumetalloidbioremediationdrivenbyphototrophicdiazotrophicbiofilm AT chaoyuanqing novelbiologicalaquacrustenhancesinsitumetalloidbioremediationdrivenbyphototrophicdiazotrophicbiofilm AT tangyetao novelbiologicalaquacrustenhancesinsitumetalloidbioremediationdrivenbyphototrophicdiazotrophicbiofilm AT qiurongliang novelbiologicalaquacrustenhancesinsitumetalloidbioremediationdrivenbyphototrophicdiazotrophicbiofilm AT wangshizhong novelbiologicalaquacrustenhancesinsitumetalloidbioremediationdrivenbyphototrophicdiazotrophicbiofilm |