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Croquemort elicits activation of the immune deficiency pathway in ticks

The immune deficiency (IMD) pathway directs host defense in arthropods upon bacterial infection. In Pancrustacea, peptidoglycan recognition proteins sense microbial moieties and initiate nuclear factor-κB-driven immune responses. Proteins that elicit the IMD pathway in non-insect arthropods remain e...

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Autores principales: O’Neal, Anya J., Singh, Nisha, Rolandelli, Agustin, Laukaitis, Hanna J., Wang, Xiaowei, Shaw, Dana K., Young, Brianna D., Narasimhan, Sukanya, Dutta, Shraboni, Snyder, Greg A., Samaddar, Sourabh, Marnin, Liron, Butler, L. Rainer, Mendes, M. Tays, Cabrera Paz, Francy E., Valencia, Luisa M., Sundberg, Eric J., Fikrig, Erol, Pal, Utpal, Weber, David J., Pedra, Joao H. F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: National Academy of Sciences 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10193931/
https://www.ncbi.nlm.nih.gov/pubmed/37155900
http://dx.doi.org/10.1073/pnas.2208673120
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author O’Neal, Anya J.
Singh, Nisha
Rolandelli, Agustin
Laukaitis, Hanna J.
Wang, Xiaowei
Shaw, Dana K.
Young, Brianna D.
Narasimhan, Sukanya
Dutta, Shraboni
Snyder, Greg A.
Samaddar, Sourabh
Marnin, Liron
Butler, L. Rainer
Mendes, M. Tays
Cabrera Paz, Francy E.
Valencia, Luisa M.
Sundberg, Eric J.
Fikrig, Erol
Pal, Utpal
Weber, David J.
Pedra, Joao H. F.
author_facet O’Neal, Anya J.
Singh, Nisha
Rolandelli, Agustin
Laukaitis, Hanna J.
Wang, Xiaowei
Shaw, Dana K.
Young, Brianna D.
Narasimhan, Sukanya
Dutta, Shraboni
Snyder, Greg A.
Samaddar, Sourabh
Marnin, Liron
Butler, L. Rainer
Mendes, M. Tays
Cabrera Paz, Francy E.
Valencia, Luisa M.
Sundberg, Eric J.
Fikrig, Erol
Pal, Utpal
Weber, David J.
Pedra, Joao H. F.
author_sort O’Neal, Anya J.
collection PubMed
description The immune deficiency (IMD) pathway directs host defense in arthropods upon bacterial infection. In Pancrustacea, peptidoglycan recognition proteins sense microbial moieties and initiate nuclear factor-κB-driven immune responses. Proteins that elicit the IMD pathway in non-insect arthropods remain elusive. Here, we show that an Ixodes scapularis homolog of croquemort (Crq), a CD36-like protein, promotes activation of the tick IMD pathway. Crq exhibits plasma membrane localization and binds the lipid agonist 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoglycerol. Crq regulates the IMD and jun N-terminal kinase signaling cascades and limits the acquisition of the Lyme disease spirochete B. burgdorferi. Additionally, nymphs silenced for crq display impaired feeding and delayed molting to adulthood due to a deficiency in ecdysteroid synthesis. Collectively, we establish a distinct mechanism for arthropod immunity outside of insects and crustaceans.
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spelling pubmed-101939312023-11-08 Croquemort elicits activation of the immune deficiency pathway in ticks O’Neal, Anya J. Singh, Nisha Rolandelli, Agustin Laukaitis, Hanna J. Wang, Xiaowei Shaw, Dana K. Young, Brianna D. Narasimhan, Sukanya Dutta, Shraboni Snyder, Greg A. Samaddar, Sourabh Marnin, Liron Butler, L. Rainer Mendes, M. Tays Cabrera Paz, Francy E. Valencia, Luisa M. Sundberg, Eric J. Fikrig, Erol Pal, Utpal Weber, David J. Pedra, Joao H. F. Proc Natl Acad Sci U S A Biological Sciences The immune deficiency (IMD) pathway directs host defense in arthropods upon bacterial infection. In Pancrustacea, peptidoglycan recognition proteins sense microbial moieties and initiate nuclear factor-κB-driven immune responses. Proteins that elicit the IMD pathway in non-insect arthropods remain elusive. Here, we show that an Ixodes scapularis homolog of croquemort (Crq), a CD36-like protein, promotes activation of the tick IMD pathway. Crq exhibits plasma membrane localization and binds the lipid agonist 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoglycerol. Crq regulates the IMD and jun N-terminal kinase signaling cascades and limits the acquisition of the Lyme disease spirochete B. burgdorferi. Additionally, nymphs silenced for crq display impaired feeding and delayed molting to adulthood due to a deficiency in ecdysteroid synthesis. Collectively, we establish a distinct mechanism for arthropod immunity outside of insects and crustaceans. National Academy of Sciences 2023-05-08 2023-05-16 /pmc/articles/PMC10193931/ /pubmed/37155900 http://dx.doi.org/10.1073/pnas.2208673120 Text en Copyright © 2023 the Author(s). Published by PNAS. https://creativecommons.org/licenses/by-nc-nd/4.0/This article is distributed under Creative Commons Attribution-NonCommercial-NoDerivatives License 4.0 (CC BY-NC-ND) (https://creativecommons.org/licenses/by-nc-nd/4.0/) .
spellingShingle Biological Sciences
O’Neal, Anya J.
Singh, Nisha
Rolandelli, Agustin
Laukaitis, Hanna J.
Wang, Xiaowei
Shaw, Dana K.
Young, Brianna D.
Narasimhan, Sukanya
Dutta, Shraboni
Snyder, Greg A.
Samaddar, Sourabh
Marnin, Liron
Butler, L. Rainer
Mendes, M. Tays
Cabrera Paz, Francy E.
Valencia, Luisa M.
Sundberg, Eric J.
Fikrig, Erol
Pal, Utpal
Weber, David J.
Pedra, Joao H. F.
Croquemort elicits activation of the immune deficiency pathway in ticks
title Croquemort elicits activation of the immune deficiency pathway in ticks
title_full Croquemort elicits activation of the immune deficiency pathway in ticks
title_fullStr Croquemort elicits activation of the immune deficiency pathway in ticks
title_full_unstemmed Croquemort elicits activation of the immune deficiency pathway in ticks
title_short Croquemort elicits activation of the immune deficiency pathway in ticks
title_sort croquemort elicits activation of the immune deficiency pathway in ticks
topic Biological Sciences
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10193931/
https://www.ncbi.nlm.nih.gov/pubmed/37155900
http://dx.doi.org/10.1073/pnas.2208673120
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