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Holotype sequencing of Silvataresholzenthali Rázuri-Gonzales, Ngera & Pauls, 2022 (Trichoptera, Pisuliidae)
While DNA barcodes are increasingly provided in descriptions of new species, the whole mitochondrial and nuclear genomes are still rarely included. This is unfortunate because whole genome sequencing of holotypes allows perpetual genetic characterization of the most representative specimen for a giv...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Pensoft Publishers
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10193998/ https://www.ncbi.nlm.nih.gov/pubmed/37213527 http://dx.doi.org/10.3897/zookeys.1159.98439 |
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author | Heckenhauer, Jacqueline Razuri-Gonzales, Ernesto Mwangi, Francois Ngera Schneider, Julio Pauls, Steffen U. |
author_facet | Heckenhauer, Jacqueline Razuri-Gonzales, Ernesto Mwangi, Francois Ngera Schneider, Julio Pauls, Steffen U. |
author_sort | Heckenhauer, Jacqueline |
collection | PubMed |
description | While DNA barcodes are increasingly provided in descriptions of new species, the whole mitochondrial and nuclear genomes are still rarely included. This is unfortunate because whole genome sequencing of holotypes allows perpetual genetic characterization of the most representative specimen for a given species. Thus, de novo genomes are invaluable additional diagnostic characters in species descriptions, provided the structural integrity of the holotype specimens remains intact. Here, we used a minimally invasive method to extract DNA of the type specimen of the recently described caddisfly species Silvataresholzenthali Rázuri-Gonzales, Ngera & Pauls, 2022 (Trichoptera: Pisuliidae) from the Democratic Republic of the Congo. A low-cost next generation sequencing strategy was used to generate the complete mitochondrial and draft nuclear genome of the holotype. The data in its current form is an important extension to the morphological species description and valuable for phylogenomic studies. |
format | Online Article Text |
id | pubmed-10193998 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Pensoft Publishers |
record_format | MEDLINE/PubMed |
spelling | pubmed-101939982023-05-19 Holotype sequencing of Silvataresholzenthali Rázuri-Gonzales, Ngera & Pauls, 2022 (Trichoptera, Pisuliidae) Heckenhauer, Jacqueline Razuri-Gonzales, Ernesto Mwangi, Francois Ngera Schneider, Julio Pauls, Steffen U. Zookeys Research Article While DNA barcodes are increasingly provided in descriptions of new species, the whole mitochondrial and nuclear genomes are still rarely included. This is unfortunate because whole genome sequencing of holotypes allows perpetual genetic characterization of the most representative specimen for a given species. Thus, de novo genomes are invaluable additional diagnostic characters in species descriptions, provided the structural integrity of the holotype specimens remains intact. Here, we used a minimally invasive method to extract DNA of the type specimen of the recently described caddisfly species Silvataresholzenthali Rázuri-Gonzales, Ngera & Pauls, 2022 (Trichoptera: Pisuliidae) from the Democratic Republic of the Congo. A low-cost next generation sequencing strategy was used to generate the complete mitochondrial and draft nuclear genome of the holotype. The data in its current form is an important extension to the morphological species description and valuable for phylogenomic studies. Pensoft Publishers 2023-04-24 /pmc/articles/PMC10193998/ /pubmed/37213527 http://dx.doi.org/10.3897/zookeys.1159.98439 Text en Jacqueline Heckenhauer, Ernesto Razuri-Gonzales, Francois Ngera Mwangi, Julio Schneider, Steffen U. Pauls https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Heckenhauer, Jacqueline Razuri-Gonzales, Ernesto Mwangi, Francois Ngera Schneider, Julio Pauls, Steffen U. Holotype sequencing of Silvataresholzenthali Rázuri-Gonzales, Ngera & Pauls, 2022 (Trichoptera, Pisuliidae) |
title | Holotype sequencing of Silvataresholzenthali Rázuri-Gonzales, Ngera & Pauls, 2022 (Trichoptera, Pisuliidae) |
title_full | Holotype sequencing of Silvataresholzenthali Rázuri-Gonzales, Ngera & Pauls, 2022 (Trichoptera, Pisuliidae) |
title_fullStr | Holotype sequencing of Silvataresholzenthali Rázuri-Gonzales, Ngera & Pauls, 2022 (Trichoptera, Pisuliidae) |
title_full_unstemmed | Holotype sequencing of Silvataresholzenthali Rázuri-Gonzales, Ngera & Pauls, 2022 (Trichoptera, Pisuliidae) |
title_short | Holotype sequencing of Silvataresholzenthali Rázuri-Gonzales, Ngera & Pauls, 2022 (Trichoptera, Pisuliidae) |
title_sort | holotype sequencing of silvataresholzenthali rázuri-gonzales, ngera & pauls, 2022 (trichoptera, pisuliidae) |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10193998/ https://www.ncbi.nlm.nih.gov/pubmed/37213527 http://dx.doi.org/10.3897/zookeys.1159.98439 |
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