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Holotype sequencing of Silvataresholzenthali Rázuri-Gonzales, Ngera & Pauls, 2022 (Trichoptera, Pisuliidae)

While DNA barcodes are increasingly provided in descriptions of new species, the whole mitochondrial and nuclear genomes are still rarely included. This is unfortunate because whole genome sequencing of holotypes allows perpetual genetic characterization of the most representative specimen for a giv...

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Autores principales: Heckenhauer, Jacqueline, Razuri-Gonzales, Ernesto, Mwangi, Francois Ngera, Schneider, Julio, Pauls, Steffen U.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Pensoft Publishers 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10193998/
https://www.ncbi.nlm.nih.gov/pubmed/37213527
http://dx.doi.org/10.3897/zookeys.1159.98439
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author Heckenhauer, Jacqueline
Razuri-Gonzales, Ernesto
Mwangi, Francois Ngera
Schneider, Julio
Pauls, Steffen U.
author_facet Heckenhauer, Jacqueline
Razuri-Gonzales, Ernesto
Mwangi, Francois Ngera
Schneider, Julio
Pauls, Steffen U.
author_sort Heckenhauer, Jacqueline
collection PubMed
description While DNA barcodes are increasingly provided in descriptions of new species, the whole mitochondrial and nuclear genomes are still rarely included. This is unfortunate because whole genome sequencing of holotypes allows perpetual genetic characterization of the most representative specimen for a given species. Thus, de novo genomes are invaluable additional diagnostic characters in species descriptions, provided the structural integrity of the holotype specimens remains intact. Here, we used a minimally invasive method to extract DNA of the type specimen of the recently described caddisfly species Silvataresholzenthali Rázuri-Gonzales, Ngera & Pauls, 2022 (Trichoptera: Pisuliidae) from the Democratic Republic of the Congo. A low-cost next generation sequencing strategy was used to generate the complete mitochondrial and draft nuclear genome of the holotype. The data in its current form is an important extension to the morphological species description and valuable for phylogenomic studies.
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spelling pubmed-101939982023-05-19 Holotype sequencing of Silvataresholzenthali Rázuri-Gonzales, Ngera & Pauls, 2022 (Trichoptera, Pisuliidae) Heckenhauer, Jacqueline Razuri-Gonzales, Ernesto Mwangi, Francois Ngera Schneider, Julio Pauls, Steffen U. Zookeys Research Article While DNA barcodes are increasingly provided in descriptions of new species, the whole mitochondrial and nuclear genomes are still rarely included. This is unfortunate because whole genome sequencing of holotypes allows perpetual genetic characterization of the most representative specimen for a given species. Thus, de novo genomes are invaluable additional diagnostic characters in species descriptions, provided the structural integrity of the holotype specimens remains intact. Here, we used a minimally invasive method to extract DNA of the type specimen of the recently described caddisfly species Silvataresholzenthali Rázuri-Gonzales, Ngera & Pauls, 2022 (Trichoptera: Pisuliidae) from the Democratic Republic of the Congo. A low-cost next generation sequencing strategy was used to generate the complete mitochondrial and draft nuclear genome of the holotype. The data in its current form is an important extension to the morphological species description and valuable for phylogenomic studies. Pensoft Publishers 2023-04-24 /pmc/articles/PMC10193998/ /pubmed/37213527 http://dx.doi.org/10.3897/zookeys.1159.98439 Text en Jacqueline Heckenhauer, Ernesto Razuri-Gonzales, Francois Ngera Mwangi, Julio Schneider, Steffen U. Pauls https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Heckenhauer, Jacqueline
Razuri-Gonzales, Ernesto
Mwangi, Francois Ngera
Schneider, Julio
Pauls, Steffen U.
Holotype sequencing of Silvataresholzenthali Rázuri-Gonzales, Ngera & Pauls, 2022 (Trichoptera, Pisuliidae)
title Holotype sequencing of Silvataresholzenthali Rázuri-Gonzales, Ngera & Pauls, 2022 (Trichoptera, Pisuliidae)
title_full Holotype sequencing of Silvataresholzenthali Rázuri-Gonzales, Ngera & Pauls, 2022 (Trichoptera, Pisuliidae)
title_fullStr Holotype sequencing of Silvataresholzenthali Rázuri-Gonzales, Ngera & Pauls, 2022 (Trichoptera, Pisuliidae)
title_full_unstemmed Holotype sequencing of Silvataresholzenthali Rázuri-Gonzales, Ngera & Pauls, 2022 (Trichoptera, Pisuliidae)
title_short Holotype sequencing of Silvataresholzenthali Rázuri-Gonzales, Ngera & Pauls, 2022 (Trichoptera, Pisuliidae)
title_sort holotype sequencing of silvataresholzenthali rázuri-gonzales, ngera & pauls, 2022 (trichoptera, pisuliidae)
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10193998/
https://www.ncbi.nlm.nih.gov/pubmed/37213527
http://dx.doi.org/10.3897/zookeys.1159.98439
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