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Characterization and the host specificity of Pet-CM3–4, a new phage infecting Cronobacter and Enterobacter strains
Bacteria belonging to Cronobacter and Enterobacter genera are opportunistic pathogens responsible for infections in immunocompromised patients including neonates. Phage therapy offers a safe method for pathogen elimination, however, phages must be well characterized before application. In the presen...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10194368/ https://www.ncbi.nlm.nih.gov/pubmed/36528171 http://dx.doi.org/10.1016/j.virusres.2022.199025 |
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author | Andrezal, Michal Oravcova, Lucia Kadličekova, Veronika Ozaee, Elham Elnwrani, Sulafa Bugala, Juraj Markuskova, Barbora Kajsik, Michal Drahovska, Hana |
author_facet | Andrezal, Michal Oravcova, Lucia Kadličekova, Veronika Ozaee, Elham Elnwrani, Sulafa Bugala, Juraj Markuskova, Barbora Kajsik, Michal Drahovska, Hana |
author_sort | Andrezal, Michal |
collection | PubMed |
description | Bacteria belonging to Cronobacter and Enterobacter genera are opportunistic pathogens responsible for infections in immunocompromised patients including neonates. Phage therapy offers a safe method for pathogen elimination, however, phages must be well characterized before application. In the present study we isolated four closely related bacteriophages from the subfamily Tevenvirinae infecting Cronobacter and Enterobacter strains. Bacteriophage Pet-CM3–4 which was isolated on C. malonaticus strain possessed broader host specificity than other three phages with primary Enterobacter hosts. Based on genome sequences all these phages have been assigned to the genus Karamvirus. We also studied factors influencing the host specificity of Pet-CM3–4 phage and its host range mutant Pet-CM3–1 and observed that a lysine to glutamine substitution in the long tail fiber adhesin was the reason of the Pet-CM3–1 reduced host specificity. By characterization of phage-resistant mutants from transposon library of C. malonaticus KMB-72 strain we identified that LPS is the receptor of both phages. C. malonaticus O:3 antigen is the receptor of Pet-CM3–1 phage and the Pet-CM3–4 phage binds to structures of the LPS core region. Obtained results will contribute to our understanding of biology and evolution of Tevenvirinae phages. |
format | Online Article Text |
id | pubmed-10194368 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-101943682023-05-19 Characterization and the host specificity of Pet-CM3–4, a new phage infecting Cronobacter and Enterobacter strains Andrezal, Michal Oravcova, Lucia Kadličekova, Veronika Ozaee, Elham Elnwrani, Sulafa Bugala, Juraj Markuskova, Barbora Kajsik, Michal Drahovska, Hana Virus Res Article Bacteria belonging to Cronobacter and Enterobacter genera are opportunistic pathogens responsible for infections in immunocompromised patients including neonates. Phage therapy offers a safe method for pathogen elimination, however, phages must be well characterized before application. In the present study we isolated four closely related bacteriophages from the subfamily Tevenvirinae infecting Cronobacter and Enterobacter strains. Bacteriophage Pet-CM3–4 which was isolated on C. malonaticus strain possessed broader host specificity than other three phages with primary Enterobacter hosts. Based on genome sequences all these phages have been assigned to the genus Karamvirus. We also studied factors influencing the host specificity of Pet-CM3–4 phage and its host range mutant Pet-CM3–1 and observed that a lysine to glutamine substitution in the long tail fiber adhesin was the reason of the Pet-CM3–1 reduced host specificity. By characterization of phage-resistant mutants from transposon library of C. malonaticus KMB-72 strain we identified that LPS is the receptor of both phages. C. malonaticus O:3 antigen is the receptor of Pet-CM3–1 phage and the Pet-CM3–4 phage binds to structures of the LPS core region. Obtained results will contribute to our understanding of biology and evolution of Tevenvirinae phages. Elsevier 2022-12-14 /pmc/articles/PMC10194368/ /pubmed/36528171 http://dx.doi.org/10.1016/j.virusres.2022.199025 Text en © 2022 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Article Andrezal, Michal Oravcova, Lucia Kadličekova, Veronika Ozaee, Elham Elnwrani, Sulafa Bugala, Juraj Markuskova, Barbora Kajsik, Michal Drahovska, Hana Characterization and the host specificity of Pet-CM3–4, a new phage infecting Cronobacter and Enterobacter strains |
title | Characterization and the host specificity of Pet-CM3–4, a new phage infecting Cronobacter and Enterobacter strains |
title_full | Characterization and the host specificity of Pet-CM3–4, a new phage infecting Cronobacter and Enterobacter strains |
title_fullStr | Characterization and the host specificity of Pet-CM3–4, a new phage infecting Cronobacter and Enterobacter strains |
title_full_unstemmed | Characterization and the host specificity of Pet-CM3–4, a new phage infecting Cronobacter and Enterobacter strains |
title_short | Characterization and the host specificity of Pet-CM3–4, a new phage infecting Cronobacter and Enterobacter strains |
title_sort | characterization and the host specificity of pet-cm3–4, a new phage infecting cronobacter and enterobacter strains |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10194368/ https://www.ncbi.nlm.nih.gov/pubmed/36528171 http://dx.doi.org/10.1016/j.virusres.2022.199025 |
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