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Expression pattern and prognostic value of key regulators for N7-methylguanosine RNA modification in prostate cancer: m7G RNA modification in PCa
Alterations in the regulators of RNA methylation modifications, such as N7-methylguanosine (m7G), have been implicated in a variety of diseases. Therefore, the analysis and identification of disease-related m7G modification regulators will accelerate advances in understanding disease pathogenesis. H...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10195147/ https://www.ncbi.nlm.nih.gov/pubmed/36810782 http://dx.doi.org/10.3724/abbs.2023017 |
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author | Zhai, Qiliang Hou, Yan Ye, Yuedian Dai, Sujuan Guo, Guangxiu Yang, Qiao Pang, Guofu Wei, Qiang |
author_facet | Zhai, Qiliang Hou, Yan Ye, Yuedian Dai, Sujuan Guo, Guangxiu Yang, Qiao Pang, Guofu Wei, Qiang |
author_sort | Zhai, Qiliang |
collection | PubMed |
description | Alterations in the regulators of RNA methylation modifications, such as N7-methylguanosine (m7G), have been implicated in a variety of diseases. Therefore, the analysis and identification of disease-related m7G modification regulators will accelerate advances in understanding disease pathogenesis. However, the implications of alterations in the regulators of m7G modifications remain poorly understood in prostate adenocarcinoma. In the present study, we analyze the expression patterns of 29 m7G RNA modification regulators in prostate adenocarcinoma using The Cancer Genome Atlas (TCGA) and perform consistent clustering analysis of differentially expressed genes (DEGs). We find that 18 m7G-related genes are differentially expressed in tumor and normal tissues. In different cluster subgroups, DEGs are mainly enriched in tumorigenesis and tumor development. Furthermore, immune analyses demonstrate that patients in cluster 1 have significantly higher scores for stromal and immune cells, such as B cells, T cells, and macrophages. Then, a TCGA-related risk model is developed and successfully validated using a Gene Expression Omnibus external dataset. Two genes ( EIF4A1 and NCBP2) are determined to be prognostically significant. Most importantly, we construct tissue microarrays from 26 tumor specimens and 20 normal specimens, and further confirm that EIF4A1 and NCBP2 are associated with tumor progression and Gleason score. Therefore, we conclude that the m7G RNA methylation regulators may be involved in the poor prognosis of patients with prostate adenocarcinoma. The results of this study may provide support for exploring the underlying molecular mechanisms of m7G regulators, especially EIF4A1 and NCBP2. |
format | Online Article Text |
id | pubmed-10195147 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-101951472023-05-19 Expression pattern and prognostic value of key regulators for N7-methylguanosine RNA modification in prostate cancer: m7G RNA modification in PCa Zhai, Qiliang Hou, Yan Ye, Yuedian Dai, Sujuan Guo, Guangxiu Yang, Qiao Pang, Guofu Wei, Qiang Acta Biochim Biophys Sin (Shanghai) Research Article Alterations in the regulators of RNA methylation modifications, such as N7-methylguanosine (m7G), have been implicated in a variety of diseases. Therefore, the analysis and identification of disease-related m7G modification regulators will accelerate advances in understanding disease pathogenesis. However, the implications of alterations in the regulators of m7G modifications remain poorly understood in prostate adenocarcinoma. In the present study, we analyze the expression patterns of 29 m7G RNA modification regulators in prostate adenocarcinoma using The Cancer Genome Atlas (TCGA) and perform consistent clustering analysis of differentially expressed genes (DEGs). We find that 18 m7G-related genes are differentially expressed in tumor and normal tissues. In different cluster subgroups, DEGs are mainly enriched in tumorigenesis and tumor development. Furthermore, immune analyses demonstrate that patients in cluster 1 have significantly higher scores for stromal and immune cells, such as B cells, T cells, and macrophages. Then, a TCGA-related risk model is developed and successfully validated using a Gene Expression Omnibus external dataset. Two genes ( EIF4A1 and NCBP2) are determined to be prognostically significant. Most importantly, we construct tissue microarrays from 26 tumor specimens and 20 normal specimens, and further confirm that EIF4A1 and NCBP2 are associated with tumor progression and Gleason score. Therefore, we conclude that the m7G RNA methylation regulators may be involved in the poor prognosis of patients with prostate adenocarcinoma. The results of this study may provide support for exploring the underlying molecular mechanisms of m7G regulators, especially EIF4A1 and NCBP2. Oxford University Press 2023-02-22 /pmc/articles/PMC10195147/ /pubmed/36810782 http://dx.doi.org/10.3724/abbs.2023017 Text en © The Author(s) 2021. 0 https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Research Article Zhai, Qiliang Hou, Yan Ye, Yuedian Dai, Sujuan Guo, Guangxiu Yang, Qiao Pang, Guofu Wei, Qiang Expression pattern and prognostic value of key regulators for N7-methylguanosine RNA modification in prostate cancer: m7G RNA modification in PCa |
title | Expression pattern and prognostic value of key regulators for N7-methylguanosine RNA modification in prostate cancer: m7G RNA modification in PCa |
title_full | Expression pattern and prognostic value of key regulators for N7-methylguanosine RNA modification in prostate cancer: m7G RNA modification in PCa |
title_fullStr | Expression pattern and prognostic value of key regulators for N7-methylguanosine RNA modification in prostate cancer: m7G RNA modification in PCa |
title_full_unstemmed | Expression pattern and prognostic value of key regulators for N7-methylguanosine RNA modification in prostate cancer: m7G RNA modification in PCa |
title_short | Expression pattern and prognostic value of key regulators for N7-methylguanosine RNA modification in prostate cancer: m7G RNA modification in PCa |
title_sort | expression pattern and prognostic value of key regulators for n7-methylguanosine rna modification in prostate cancer: m7g rna modification in pca |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10195147/ https://www.ncbi.nlm.nih.gov/pubmed/36810782 http://dx.doi.org/10.3724/abbs.2023017 |
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