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An organ-on-chip device with integrated charge sensors and recording microelectrodes
Continuous monitoring of tissue microphysiology is a key enabling feature of the organ-on-chip (OoC) approach for in vitro drug screening and disease modeling. Integrated sensing units are particularly convenient for microenvironmental monitoring. However, sensitive in vitro and real-time measuremen...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10195821/ https://www.ncbi.nlm.nih.gov/pubmed/37202451 http://dx.doi.org/10.1038/s41598-023-34786-5 |
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author | Aydogmus, Hande Hu, Michel Ivancevic, Lovro Frimat, Jean-Philippe van den Maagdenberg, Arn M. J. M. Sarro, Pasqualina M. Mastrangeli, Massimo |
author_facet | Aydogmus, Hande Hu, Michel Ivancevic, Lovro Frimat, Jean-Philippe van den Maagdenberg, Arn M. J. M. Sarro, Pasqualina M. Mastrangeli, Massimo |
author_sort | Aydogmus, Hande |
collection | PubMed |
description | Continuous monitoring of tissue microphysiology is a key enabling feature of the organ-on-chip (OoC) approach for in vitro drug screening and disease modeling. Integrated sensing units are particularly convenient for microenvironmental monitoring. However, sensitive in vitro and real-time measurements are challenging due to the inherently small size of OoC devices, the characteristics of commonly used materials, and external hardware setups required to support the sensing units. Here we propose a silicon-polymer hybrid OoC device that encompasses transparency and biocompatibility of polymers at the sensing area, and has the inherently superior electrical characteristics and ability to house active electronics of silicon. This multi-modal device includes two sensing units. The first unit consists of a floating-gate field-effect transistor (FG-FET), which is used to monitor changes in pH in the sensing area. The threshold voltage of the FG-FET is regulated by a capacitively-coupled gate and by the changes in charge concentration in close proximity to the extension of the floating gate, which functions as the sensing electrode. The second unit uses the extension of the FG as microelectrode, in order to monitor the action potential of electrically active cells. The layout of the chip and its packaging are compatible with multi-electrode array measurement setups, which are commonly used in electrophysiology labs. The multi-functional sensing is demonstrated by monitoring the growth of induced pluripotent stem cell-derived cortical neurons. Our multi-modal sensor is a milestone in combined monitoring of different, physiologically-relevant parameters on the same device for future OoC platforms. |
format | Online Article Text |
id | pubmed-10195821 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-101958212023-05-20 An organ-on-chip device with integrated charge sensors and recording microelectrodes Aydogmus, Hande Hu, Michel Ivancevic, Lovro Frimat, Jean-Philippe van den Maagdenberg, Arn M. J. M. Sarro, Pasqualina M. Mastrangeli, Massimo Sci Rep Article Continuous monitoring of tissue microphysiology is a key enabling feature of the organ-on-chip (OoC) approach for in vitro drug screening and disease modeling. Integrated sensing units are particularly convenient for microenvironmental monitoring. However, sensitive in vitro and real-time measurements are challenging due to the inherently small size of OoC devices, the characteristics of commonly used materials, and external hardware setups required to support the sensing units. Here we propose a silicon-polymer hybrid OoC device that encompasses transparency and biocompatibility of polymers at the sensing area, and has the inherently superior electrical characteristics and ability to house active electronics of silicon. This multi-modal device includes two sensing units. The first unit consists of a floating-gate field-effect transistor (FG-FET), which is used to monitor changes in pH in the sensing area. The threshold voltage of the FG-FET is regulated by a capacitively-coupled gate and by the changes in charge concentration in close proximity to the extension of the floating gate, which functions as the sensing electrode. The second unit uses the extension of the FG as microelectrode, in order to monitor the action potential of electrically active cells. The layout of the chip and its packaging are compatible with multi-electrode array measurement setups, which are commonly used in electrophysiology labs. The multi-functional sensing is demonstrated by monitoring the growth of induced pluripotent stem cell-derived cortical neurons. Our multi-modal sensor is a milestone in combined monitoring of different, physiologically-relevant parameters on the same device for future OoC platforms. Nature Publishing Group UK 2023-05-18 /pmc/articles/PMC10195821/ /pubmed/37202451 http://dx.doi.org/10.1038/s41598-023-34786-5 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Aydogmus, Hande Hu, Michel Ivancevic, Lovro Frimat, Jean-Philippe van den Maagdenberg, Arn M. J. M. Sarro, Pasqualina M. Mastrangeli, Massimo An organ-on-chip device with integrated charge sensors and recording microelectrodes |
title | An organ-on-chip device with integrated charge sensors and recording microelectrodes |
title_full | An organ-on-chip device with integrated charge sensors and recording microelectrodes |
title_fullStr | An organ-on-chip device with integrated charge sensors and recording microelectrodes |
title_full_unstemmed | An organ-on-chip device with integrated charge sensors and recording microelectrodes |
title_short | An organ-on-chip device with integrated charge sensors and recording microelectrodes |
title_sort | organ-on-chip device with integrated charge sensors and recording microelectrodes |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10195821/ https://www.ncbi.nlm.nih.gov/pubmed/37202451 http://dx.doi.org/10.1038/s41598-023-34786-5 |
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