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Phrenic nerve stimulation mitigates hippocampal and brainstem inflammation in an ARDS model

Rationale: In porcine healthy-lung and moderate acute respiratory distress syndrome (ARDS) models, groups that received phrenic nerve stimulation (PNS) with mechanical ventilation (MV) showed lower hippocampal apoptosis, and microglia and astrocyte percentages than MV alone. Objectives: Explore whet...

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Autores principales: Bassi, Thiago G., Rohrs, Elizabeth C., Fernandez, Karl C., Ornowska, Marlena, Nicholas, Michelle, Wittmann, Jessica, Gani, Matt, Evans, Doug, Reynolds, Steven C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10196250/
https://www.ncbi.nlm.nih.gov/pubmed/37215178
http://dx.doi.org/10.3389/fphys.2023.1182505
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author Bassi, Thiago G.
Rohrs, Elizabeth C.
Fernandez, Karl C.
Ornowska, Marlena
Nicholas, Michelle
Wittmann, Jessica
Gani, Matt
Evans, Doug
Reynolds, Steven C.
author_facet Bassi, Thiago G.
Rohrs, Elizabeth C.
Fernandez, Karl C.
Ornowska, Marlena
Nicholas, Michelle
Wittmann, Jessica
Gani, Matt
Evans, Doug
Reynolds, Steven C.
author_sort Bassi, Thiago G.
collection PubMed
description Rationale: In porcine healthy-lung and moderate acute respiratory distress syndrome (ARDS) models, groups that received phrenic nerve stimulation (PNS) with mechanical ventilation (MV) showed lower hippocampal apoptosis, and microglia and astrocyte percentages than MV alone. Objectives: Explore whether PNS in combination with MV for 12 h leads to differences in hippocampal and brainstem tissue concentrations of inflammatory and synaptic markers compared to MV-only animals. Methods: Compare tissue concentrations of inflammatory markers (IL-1α, IL-1β, IL-6, IL-8, IL-10, IFN-γ, TNFα and GM-CSF), pre-synaptic markers (synapsin and synaptophysin) and post-synaptic markers (disc-large-homolog 4, N-methyl-D-aspartate receptors 2A and 2B) in the hippocampus and brainstem in three groups of mechanically ventilated pigs with injured lungs: MV only (MV), MV plus PNS every other breath (MV + PNS50%), and MV plus PNS every breath (MV + PNS100%). MV settings in volume control were tidal volume 8 ml/kg, and positive end-expiratory pressure 5 cmH(2)O. Moderate ARDS was achieved by infusing oleic acid into the pulmonary artery. Measurements and Main Results: Hippocampal concentrations of GM-CSF, N-methyl-D-aspartate receptor 2B, and synaptophysin were greater in the MV + PNS100% group compared to the MV group, p = 0.0199, p = 0.0175, and p = 0.0479, respectively. The MV + PNS100% group had lower brainstem concentrations of IL-1β, and IL-8 than the MV group, p = 0.0194, and p = 0.0319, respectively; and greater brainstem concentrations of IFN-γ and N-methyl-D-aspartate receptor 2A than the MV group, p = 0.0329, and p = 0.0125, respectively. Conclusion: In a moderate-ARDS porcine model, MV is associated with hippocampal and brainstem inflammation, and phrenic nerve stimulation on every breath mitigates that inflammation.
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spelling pubmed-101962502023-05-20 Phrenic nerve stimulation mitigates hippocampal and brainstem inflammation in an ARDS model Bassi, Thiago G. Rohrs, Elizabeth C. Fernandez, Karl C. Ornowska, Marlena Nicholas, Michelle Wittmann, Jessica Gani, Matt Evans, Doug Reynolds, Steven C. Front Physiol Physiology Rationale: In porcine healthy-lung and moderate acute respiratory distress syndrome (ARDS) models, groups that received phrenic nerve stimulation (PNS) with mechanical ventilation (MV) showed lower hippocampal apoptosis, and microglia and astrocyte percentages than MV alone. Objectives: Explore whether PNS in combination with MV for 12 h leads to differences in hippocampal and brainstem tissue concentrations of inflammatory and synaptic markers compared to MV-only animals. Methods: Compare tissue concentrations of inflammatory markers (IL-1α, IL-1β, IL-6, IL-8, IL-10, IFN-γ, TNFα and GM-CSF), pre-synaptic markers (synapsin and synaptophysin) and post-synaptic markers (disc-large-homolog 4, N-methyl-D-aspartate receptors 2A and 2B) in the hippocampus and brainstem in three groups of mechanically ventilated pigs with injured lungs: MV only (MV), MV plus PNS every other breath (MV + PNS50%), and MV plus PNS every breath (MV + PNS100%). MV settings in volume control were tidal volume 8 ml/kg, and positive end-expiratory pressure 5 cmH(2)O. Moderate ARDS was achieved by infusing oleic acid into the pulmonary artery. Measurements and Main Results: Hippocampal concentrations of GM-CSF, N-methyl-D-aspartate receptor 2B, and synaptophysin were greater in the MV + PNS100% group compared to the MV group, p = 0.0199, p = 0.0175, and p = 0.0479, respectively. The MV + PNS100% group had lower brainstem concentrations of IL-1β, and IL-8 than the MV group, p = 0.0194, and p = 0.0319, respectively; and greater brainstem concentrations of IFN-γ and N-methyl-D-aspartate receptor 2A than the MV group, p = 0.0329, and p = 0.0125, respectively. Conclusion: In a moderate-ARDS porcine model, MV is associated with hippocampal and brainstem inflammation, and phrenic nerve stimulation on every breath mitigates that inflammation. Frontiers Media S.A. 2023-05-05 /pmc/articles/PMC10196250/ /pubmed/37215178 http://dx.doi.org/10.3389/fphys.2023.1182505 Text en Copyright © 2023 Bassi, Rohrs, Fernandez, Ornowska, Nicholas, Wittmann, Gani, Evans and Reynolds. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Physiology
Bassi, Thiago G.
Rohrs, Elizabeth C.
Fernandez, Karl C.
Ornowska, Marlena
Nicholas, Michelle
Wittmann, Jessica
Gani, Matt
Evans, Doug
Reynolds, Steven C.
Phrenic nerve stimulation mitigates hippocampal and brainstem inflammation in an ARDS model
title Phrenic nerve stimulation mitigates hippocampal and brainstem inflammation in an ARDS model
title_full Phrenic nerve stimulation mitigates hippocampal and brainstem inflammation in an ARDS model
title_fullStr Phrenic nerve stimulation mitigates hippocampal and brainstem inflammation in an ARDS model
title_full_unstemmed Phrenic nerve stimulation mitigates hippocampal and brainstem inflammation in an ARDS model
title_short Phrenic nerve stimulation mitigates hippocampal and brainstem inflammation in an ARDS model
title_sort phrenic nerve stimulation mitigates hippocampal and brainstem inflammation in an ards model
topic Physiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10196250/
https://www.ncbi.nlm.nih.gov/pubmed/37215178
http://dx.doi.org/10.3389/fphys.2023.1182505
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