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Comparison of muscle metabolomics between two Chinese horse breeds

With their enormous muscle mass and athletic ability, horses are well-positioned as model organisms for understanding muscle metabolism. There are two different types of horse breeds—Guanzhong (GZ) horses, an athletic breed with a larger body height (~148.7 cm), and the Ningqiang pony (NQ) horses, a...

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Autores principales: Meng, Sihan, Zhang, Yanli, Lv, Shipeng, Zhang, Zhengkai, Liu, Xuexue, Jiang, Lin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10196265/
https://www.ncbi.nlm.nih.gov/pubmed/37215482
http://dx.doi.org/10.3389/fvets.2023.1162953
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author Meng, Sihan
Zhang, Yanli
Lv, Shipeng
Zhang, Zhengkai
Liu, Xuexue
Jiang, Lin
author_facet Meng, Sihan
Zhang, Yanli
Lv, Shipeng
Zhang, Zhengkai
Liu, Xuexue
Jiang, Lin
author_sort Meng, Sihan
collection PubMed
description With their enormous muscle mass and athletic ability, horses are well-positioned as model organisms for understanding muscle metabolism. There are two different types of horse breeds—Guanzhong (GZ) horses, an athletic breed with a larger body height (~148.7 cm), and the Ningqiang pony (NQ) horses, a lower height breed generally used for ornamental purposes—both inhabited in the same region of China with obvious differences in muscle content. The main objective of this study was to evaluate the breed-specific mechanisms controlling muscle metabolism. In this study, we observed muscle glycogen, enzyme activities, and LC–MS/MS untargeted metabolomics in the gluteus medius muscle of six, each of GZ and NQ horses, to explore differentiated metabolites that are related to the development of two muscles. As expected, the glycogen content, citrate synthase, and hexokinase activity of muscle were significantly higher in GZ horses. To alleviate the false positive rate, we used both MS1 and MS2 ions for metabolite classification and differential analysis. As a result, a total of 51,535 MS1 and 541 MS2 metabolites were identified, and these metabolites can separate these two groups from each other. Notably, 40% of these metabolites were clustered into lipids and lipid-like molecules. Furthermore, 13 significant metabolites were differentially detected between GZ and NQ horses (fold change [FC] value ≥ 2, variable important in projection value ≥1, and Q value ≤ 0.05). They are primarily clustered into glutathione metabolism (GSH, p = 0.01), taurine, and hypotaurine metabolism (p < 0.05) pathways. Seven of the 13 metabolites were also found in thoroughbred racing horses, suggesting that metabolites related to antioxidants, amino acids, and lipids played a key role in the development of skeleton muscle in horses. Those metabolites related to muscle development shed a light on racing horses' routine maintenance and improvement of athletic performance.
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spelling pubmed-101962652023-05-20 Comparison of muscle metabolomics between two Chinese horse breeds Meng, Sihan Zhang, Yanli Lv, Shipeng Zhang, Zhengkai Liu, Xuexue Jiang, Lin Front Vet Sci Veterinary Science With their enormous muscle mass and athletic ability, horses are well-positioned as model organisms for understanding muscle metabolism. There are two different types of horse breeds—Guanzhong (GZ) horses, an athletic breed with a larger body height (~148.7 cm), and the Ningqiang pony (NQ) horses, a lower height breed generally used for ornamental purposes—both inhabited in the same region of China with obvious differences in muscle content. The main objective of this study was to evaluate the breed-specific mechanisms controlling muscle metabolism. In this study, we observed muscle glycogen, enzyme activities, and LC–MS/MS untargeted metabolomics in the gluteus medius muscle of six, each of GZ and NQ horses, to explore differentiated metabolites that are related to the development of two muscles. As expected, the glycogen content, citrate synthase, and hexokinase activity of muscle were significantly higher in GZ horses. To alleviate the false positive rate, we used both MS1 and MS2 ions for metabolite classification and differential analysis. As a result, a total of 51,535 MS1 and 541 MS2 metabolites were identified, and these metabolites can separate these two groups from each other. Notably, 40% of these metabolites were clustered into lipids and lipid-like molecules. Furthermore, 13 significant metabolites were differentially detected between GZ and NQ horses (fold change [FC] value ≥ 2, variable important in projection value ≥1, and Q value ≤ 0.05). They are primarily clustered into glutathione metabolism (GSH, p = 0.01), taurine, and hypotaurine metabolism (p < 0.05) pathways. Seven of the 13 metabolites were also found in thoroughbred racing horses, suggesting that metabolites related to antioxidants, amino acids, and lipids played a key role in the development of skeleton muscle in horses. Those metabolites related to muscle development shed a light on racing horses' routine maintenance and improvement of athletic performance. Frontiers Media S.A. 2023-05-05 /pmc/articles/PMC10196265/ /pubmed/37215482 http://dx.doi.org/10.3389/fvets.2023.1162953 Text en Copyright © 2023 Meng, Zhang, Lv, Zhang, Liu and Jiang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Veterinary Science
Meng, Sihan
Zhang, Yanli
Lv, Shipeng
Zhang, Zhengkai
Liu, Xuexue
Jiang, Lin
Comparison of muscle metabolomics between two Chinese horse breeds
title Comparison of muscle metabolomics between two Chinese horse breeds
title_full Comparison of muscle metabolomics between two Chinese horse breeds
title_fullStr Comparison of muscle metabolomics between two Chinese horse breeds
title_full_unstemmed Comparison of muscle metabolomics between two Chinese horse breeds
title_short Comparison of muscle metabolomics between two Chinese horse breeds
title_sort comparison of muscle metabolomics between two chinese horse breeds
topic Veterinary Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10196265/
https://www.ncbi.nlm.nih.gov/pubmed/37215482
http://dx.doi.org/10.3389/fvets.2023.1162953
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