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Advanced Non-small Cell Lung Cancer: EGFR Mutation Analysis Using Pyrosequencing and the Fully Automated qPCR-Based Idylla(TM) System
BACKGROUND: Epidermal growth factor receptor (EGFR) mutation status is of a major clinical significance in non-small cell lung cancer (NSCLC) management, as it guides therapeutic decision making to target patients for a better response to therapy. This implicates the introduction of EGFR mutation an...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
SAGE Publications
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10196548/ https://www.ncbi.nlm.nih.gov/pubmed/37196225 http://dx.doi.org/10.1177/10732748231177538 |
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author | Boustany, Youssra Laraqui, Abdelilah El Zaitouni, Sara Ghaouti, Merieme Benzekri, Asmae Kettani, Fouad Oukabli, Mohammed Ennibi, Khalid Belkadi, Bouchra Sekhsokh, Yassine |
author_facet | Boustany, Youssra Laraqui, Abdelilah El Zaitouni, Sara Ghaouti, Merieme Benzekri, Asmae Kettani, Fouad Oukabli, Mohammed Ennibi, Khalid Belkadi, Bouchra Sekhsokh, Yassine |
author_sort | Boustany, Youssra |
collection | PubMed |
description | BACKGROUND: Epidermal growth factor receptor (EGFR) mutation status is of a major clinical significance in non-small cell lung cancer (NSCLC) management, as it guides therapeutic decision making to target patients for a better response to therapy. This implicates the introduction of EGFR mutation analysis as the standard of care for Moroccan NSCLC patients, which in itself entails the implementation of targeted methods for routine EGFR mutation analysis in our laboratories. In this study, we aimed to present 2 targeted methods for EGFR mutation identification and to determine the prevalence and spectrum of EGFR mutations in NSCLC Moroccan patients. METHODS: A retrospective investigation of a cohort of 340 patients was undertaken to analyze somatic EGFR mutations in exons 18 to 21 using pyrosequencing and the Idylla(TM) system. RESULTS: Of the enrolled patients, 70.9% were males and 29.1% were females. Predominately, 92% of cases had adenocarcinoma, and 53.7% of patients self-reported a history of smoking. Overall, 73 patients (21.7%) harbored an EGFR mutation, the most prevalent of which were the exon 19 deletions (53.4%) followed by exon 21 substitutions (31%). Exon 18 mutations and exon 20 alterations occurred in 8.1% and 6.7% of the positive EGFR mutation cases, respectively. Of the analyzed cases, all of the EGFR-mutated patients had adenocarcinoma. EGFR mutation prevalence was significantly higher in females (females vs males: 38.4% vs 14.5%, P < .001) and non-smokers (non-smokers vs non-smokers: 36% vs 10.3%, P < .001). The featured pyrosequencing and the Idylla(TM) system are targeted methods endowed with high sensitivity and specificity as well as other compelling characteristics which make them great options for routine EGFR mutation testing for advanced NSCLC patients. CONCLUSION: These findings underline the imperious need for implementing quick and efficient targeted methods for routine EGFR mutation testing among NSCLC patients, which is particularly useful in determining patients who are more likely to benefit from targeted therapy. |
format | Online Article Text |
id | pubmed-10196548 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | SAGE Publications |
record_format | MEDLINE/PubMed |
spelling | pubmed-101965482023-05-20 Advanced Non-small Cell Lung Cancer: EGFR Mutation Analysis Using Pyrosequencing and the Fully Automated qPCR-Based Idylla(TM) System Boustany, Youssra Laraqui, Abdelilah El Zaitouni, Sara Ghaouti, Merieme Benzekri, Asmae Kettani, Fouad Oukabli, Mohammed Ennibi, Khalid Belkadi, Bouchra Sekhsokh, Yassine Cancer Control Original Research Article BACKGROUND: Epidermal growth factor receptor (EGFR) mutation status is of a major clinical significance in non-small cell lung cancer (NSCLC) management, as it guides therapeutic decision making to target patients for a better response to therapy. This implicates the introduction of EGFR mutation analysis as the standard of care for Moroccan NSCLC patients, which in itself entails the implementation of targeted methods for routine EGFR mutation analysis in our laboratories. In this study, we aimed to present 2 targeted methods for EGFR mutation identification and to determine the prevalence and spectrum of EGFR mutations in NSCLC Moroccan patients. METHODS: A retrospective investigation of a cohort of 340 patients was undertaken to analyze somatic EGFR mutations in exons 18 to 21 using pyrosequencing and the Idylla(TM) system. RESULTS: Of the enrolled patients, 70.9% were males and 29.1% were females. Predominately, 92% of cases had adenocarcinoma, and 53.7% of patients self-reported a history of smoking. Overall, 73 patients (21.7%) harbored an EGFR mutation, the most prevalent of which were the exon 19 deletions (53.4%) followed by exon 21 substitutions (31%). Exon 18 mutations and exon 20 alterations occurred in 8.1% and 6.7% of the positive EGFR mutation cases, respectively. Of the analyzed cases, all of the EGFR-mutated patients had adenocarcinoma. EGFR mutation prevalence was significantly higher in females (females vs males: 38.4% vs 14.5%, P < .001) and non-smokers (non-smokers vs non-smokers: 36% vs 10.3%, P < .001). The featured pyrosequencing and the Idylla(TM) system are targeted methods endowed with high sensitivity and specificity as well as other compelling characteristics which make them great options for routine EGFR mutation testing for advanced NSCLC patients. CONCLUSION: These findings underline the imperious need for implementing quick and efficient targeted methods for routine EGFR mutation testing among NSCLC patients, which is particularly useful in determining patients who are more likely to benefit from targeted therapy. SAGE Publications 2023-05-17 /pmc/articles/PMC10196548/ /pubmed/37196225 http://dx.doi.org/10.1177/10732748231177538 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by-nc/4.0/This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage). |
spellingShingle | Original Research Article Boustany, Youssra Laraqui, Abdelilah El Zaitouni, Sara Ghaouti, Merieme Benzekri, Asmae Kettani, Fouad Oukabli, Mohammed Ennibi, Khalid Belkadi, Bouchra Sekhsokh, Yassine Advanced Non-small Cell Lung Cancer: EGFR Mutation Analysis Using Pyrosequencing and the Fully Automated qPCR-Based Idylla(TM) System |
title | Advanced Non-small Cell Lung Cancer: EGFR Mutation Analysis Using
Pyrosequencing and the Fully Automated qPCR-Based Idylla(TM)
System |
title_full | Advanced Non-small Cell Lung Cancer: EGFR Mutation Analysis Using
Pyrosequencing and the Fully Automated qPCR-Based Idylla(TM)
System |
title_fullStr | Advanced Non-small Cell Lung Cancer: EGFR Mutation Analysis Using
Pyrosequencing and the Fully Automated qPCR-Based Idylla(TM)
System |
title_full_unstemmed | Advanced Non-small Cell Lung Cancer: EGFR Mutation Analysis Using
Pyrosequencing and the Fully Automated qPCR-Based Idylla(TM)
System |
title_short | Advanced Non-small Cell Lung Cancer: EGFR Mutation Analysis Using
Pyrosequencing and the Fully Automated qPCR-Based Idylla(TM)
System |
title_sort | advanced non-small cell lung cancer: egfr mutation analysis using
pyrosequencing and the fully automated qpcr-based idylla(tm)
system |
topic | Original Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10196548/ https://www.ncbi.nlm.nih.gov/pubmed/37196225 http://dx.doi.org/10.1177/10732748231177538 |
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