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Rapid diagnosis of different goose astrovirus genotypes with Taqman-based duplex real-time quantitative PCR
The epidemic of goose astrovirus (GoAstV) caused huge losses to the poultry industry. Epidemiological studies in China revealed 2 circulating genotypes of GoAstV, but there is a lack of differential diagnosis tools. By analyzing all published genomes of GoAstV, this study designed specific PCR prime...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10196781/ https://www.ncbi.nlm.nih.gov/pubmed/37167886 http://dx.doi.org/10.1016/j.psj.2023.102730 |
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author | Li, Haiqin Kang, Zhaofeng Wan, Chunhe Zhang, Fanfan Tan, Meifang Zeng, Yanbing Wu, Chengcheng Huang, Yu Su, Qi Guo, Xiaoquan |
author_facet | Li, Haiqin Kang, Zhaofeng Wan, Chunhe Zhang, Fanfan Tan, Meifang Zeng, Yanbing Wu, Chengcheng Huang, Yu Su, Qi Guo, Xiaoquan |
author_sort | Li, Haiqin |
collection | PubMed |
description | The epidemic of goose astrovirus (GoAstV) caused huge losses to the poultry industry. Epidemiological studies in China revealed 2 circulating genotypes of GoAstV, but there is a lack of differential diagnosis tools. By analyzing all published genomes of GoAstV, this study designed specific PCR primers and Taqman probes to recognize different genotypes of GoAstV. After optimization and verification, this study developed a Taqman-based real-time quantitative PCR method that is capable of differential diagnosis. The established qPCR exhibited detection limitations of 100 copies/μL or 10 copies/μL, respectively, for GoAstV genotype 1 and genotype 2, and showed no false positive for other common avian viruses. This method was then used to analyze 72 samples collected from different regions in Jiangxi, and the results were verified by genome sequencing and phylogenetic analysis. These results revealed a complex coinfection of GoAstV different genotypes in China, highlighting the importance of long-term focus on the prevalence and genome evolution of GoAstV. |
format | Online Article Text |
id | pubmed-10196781 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-101967812023-05-20 Rapid diagnosis of different goose astrovirus genotypes with Taqman-based duplex real-time quantitative PCR Li, Haiqin Kang, Zhaofeng Wan, Chunhe Zhang, Fanfan Tan, Meifang Zeng, Yanbing Wu, Chengcheng Huang, Yu Su, Qi Guo, Xiaoquan Poult Sci IMMUNOLOGY, HEALTH AND DISEASE The epidemic of goose astrovirus (GoAstV) caused huge losses to the poultry industry. Epidemiological studies in China revealed 2 circulating genotypes of GoAstV, but there is a lack of differential diagnosis tools. By analyzing all published genomes of GoAstV, this study designed specific PCR primers and Taqman probes to recognize different genotypes of GoAstV. After optimization and verification, this study developed a Taqman-based real-time quantitative PCR method that is capable of differential diagnosis. The established qPCR exhibited detection limitations of 100 copies/μL or 10 copies/μL, respectively, for GoAstV genotype 1 and genotype 2, and showed no false positive for other common avian viruses. This method was then used to analyze 72 samples collected from different regions in Jiangxi, and the results were verified by genome sequencing and phylogenetic analysis. These results revealed a complex coinfection of GoAstV different genotypes in China, highlighting the importance of long-term focus on the prevalence and genome evolution of GoAstV. Elsevier 2023-04-20 /pmc/articles/PMC10196781/ /pubmed/37167886 http://dx.doi.org/10.1016/j.psj.2023.102730 Text en © 2023 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | IMMUNOLOGY, HEALTH AND DISEASE Li, Haiqin Kang, Zhaofeng Wan, Chunhe Zhang, Fanfan Tan, Meifang Zeng, Yanbing Wu, Chengcheng Huang, Yu Su, Qi Guo, Xiaoquan Rapid diagnosis of different goose astrovirus genotypes with Taqman-based duplex real-time quantitative PCR |
title | Rapid diagnosis of different goose astrovirus genotypes with Taqman-based duplex real-time quantitative PCR |
title_full | Rapid diagnosis of different goose astrovirus genotypes with Taqman-based duplex real-time quantitative PCR |
title_fullStr | Rapid diagnosis of different goose astrovirus genotypes with Taqman-based duplex real-time quantitative PCR |
title_full_unstemmed | Rapid diagnosis of different goose astrovirus genotypes with Taqman-based duplex real-time quantitative PCR |
title_short | Rapid diagnosis of different goose astrovirus genotypes with Taqman-based duplex real-time quantitative PCR |
title_sort | rapid diagnosis of different goose astrovirus genotypes with taqman-based duplex real-time quantitative pcr |
topic | IMMUNOLOGY, HEALTH AND DISEASE |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10196781/ https://www.ncbi.nlm.nih.gov/pubmed/37167886 http://dx.doi.org/10.1016/j.psj.2023.102730 |
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