Cargando…

Strategies for improving the 3D printability of decellularized extracellular matrix bioink

3D bioprinting is a revolutionary technology capable of replicating native tissue and organ microenvironments by precisely placing cells into 3D structures using bioinks. However, acquiring the ideal bioink to manufacture biomimetic constructs is challenging. A natural extracellular matrix (ECM) is...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhang, Huihui, Wang, Yilin, Zheng, Zijun, Wei, Xuerong, Chen, Lianglong, Wu, Yaobin, Huang, Wenhua, Yang, Lei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Ivyspring International Publisher 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10196833/
https://www.ncbi.nlm.nih.gov/pubmed/37215563
http://dx.doi.org/10.7150/thno.81785
_version_ 1785044429306855424
author Zhang, Huihui
Wang, Yilin
Zheng, Zijun
Wei, Xuerong
Chen, Lianglong
Wu, Yaobin
Huang, Wenhua
Yang, Lei
author_facet Zhang, Huihui
Wang, Yilin
Zheng, Zijun
Wei, Xuerong
Chen, Lianglong
Wu, Yaobin
Huang, Wenhua
Yang, Lei
author_sort Zhang, Huihui
collection PubMed
description 3D bioprinting is a revolutionary technology capable of replicating native tissue and organ microenvironments by precisely placing cells into 3D structures using bioinks. However, acquiring the ideal bioink to manufacture biomimetic constructs is challenging. A natural extracellular matrix (ECM) is an organ-specific material that provides physical, chemical, biological, and mechanical cues that are hard to mimic using a small number of components. Organ-derived decellularized ECM (dECM) bioink is revolutionary and has optimal biomimetic properties. However, dECM is always "non-printable" owing to its poor mechanical properties. Recent studies have focused on strategies to improve the 3D printability of dECM bioink. In this review, we highlight the decellularization methods and procedures used to produce these bioinks, effective methods to improve their printability, and recent advances in tissue regeneration using dECM-based bioinks. Finally, we discuss the challenges associated with manufacturing dECM bioinks and their potential large-scale applications.
format Online
Article
Text
id pubmed-10196833
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Ivyspring International Publisher
record_format MEDLINE/PubMed
spelling pubmed-101968332023-05-20 Strategies for improving the 3D printability of decellularized extracellular matrix bioink Zhang, Huihui Wang, Yilin Zheng, Zijun Wei, Xuerong Chen, Lianglong Wu, Yaobin Huang, Wenhua Yang, Lei Theranostics Review 3D bioprinting is a revolutionary technology capable of replicating native tissue and organ microenvironments by precisely placing cells into 3D structures using bioinks. However, acquiring the ideal bioink to manufacture biomimetic constructs is challenging. A natural extracellular matrix (ECM) is an organ-specific material that provides physical, chemical, biological, and mechanical cues that are hard to mimic using a small number of components. Organ-derived decellularized ECM (dECM) bioink is revolutionary and has optimal biomimetic properties. However, dECM is always "non-printable" owing to its poor mechanical properties. Recent studies have focused on strategies to improve the 3D printability of dECM bioink. In this review, we highlight the decellularization methods and procedures used to produce these bioinks, effective methods to improve their printability, and recent advances in tissue regeneration using dECM-based bioinks. Finally, we discuss the challenges associated with manufacturing dECM bioinks and their potential large-scale applications. Ivyspring International Publisher 2023-04-23 /pmc/articles/PMC10196833/ /pubmed/37215563 http://dx.doi.org/10.7150/thno.81785 Text en © The author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/). See http://ivyspring.com/terms for full terms and conditions.
spellingShingle Review
Zhang, Huihui
Wang, Yilin
Zheng, Zijun
Wei, Xuerong
Chen, Lianglong
Wu, Yaobin
Huang, Wenhua
Yang, Lei
Strategies for improving the 3D printability of decellularized extracellular matrix bioink
title Strategies for improving the 3D printability of decellularized extracellular matrix bioink
title_full Strategies for improving the 3D printability of decellularized extracellular matrix bioink
title_fullStr Strategies for improving the 3D printability of decellularized extracellular matrix bioink
title_full_unstemmed Strategies for improving the 3D printability of decellularized extracellular matrix bioink
title_short Strategies for improving the 3D printability of decellularized extracellular matrix bioink
title_sort strategies for improving the 3d printability of decellularized extracellular matrix bioink
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10196833/
https://www.ncbi.nlm.nih.gov/pubmed/37215563
http://dx.doi.org/10.7150/thno.81785
work_keys_str_mv AT zhanghuihui strategiesforimprovingthe3dprintabilityofdecellularizedextracellularmatrixbioink
AT wangyilin strategiesforimprovingthe3dprintabilityofdecellularizedextracellularmatrixbioink
AT zhengzijun strategiesforimprovingthe3dprintabilityofdecellularizedextracellularmatrixbioink
AT weixuerong strategiesforimprovingthe3dprintabilityofdecellularizedextracellularmatrixbioink
AT chenlianglong strategiesforimprovingthe3dprintabilityofdecellularizedextracellularmatrixbioink
AT wuyaobin strategiesforimprovingthe3dprintabilityofdecellularizedextracellularmatrixbioink
AT huangwenhua strategiesforimprovingthe3dprintabilityofdecellularizedextracellularmatrixbioink
AT yanglei strategiesforimprovingthe3dprintabilityofdecellularizedextracellularmatrixbioink