Development of high affinity broadly reactive aptamers for spike protein of multiple SARS-CoV-2 variants

We have developed broadly reactive aptamers against multiple variants by alternating the target between spike proteins from different SARS-CoV-2 variants during the selection process. In this process we have developed aptamers which can recognise all variants, from the original wild-type ‘Wuhan’ str...

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Detalles Bibliográficos
Autores principales: Le, Thao T., Benton, Donald J., Wrobel, Antoni G., Gamblin, Steven J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2023
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10197177/
https://www.ncbi.nlm.nih.gov/pubmed/37213341
http://dx.doi.org/10.1039/d3ra01382k
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author Le, Thao T.
Benton, Donald J.
Wrobel, Antoni G.
Gamblin, Steven J.
author_facet Le, Thao T.
Benton, Donald J.
Wrobel, Antoni G.
Gamblin, Steven J.
author_sort Le, Thao T.
collection PubMed
description We have developed broadly reactive aptamers against multiple variants by alternating the target between spike proteins from different SARS-CoV-2 variants during the selection process. In this process we have developed aptamers which can recognise all variants, from the original wild-type ‘Wuhan’ strain to Omicron, with high affinity (K(d) values in the pM range).
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spelling pubmed-101971772023-05-20 Development of high affinity broadly reactive aptamers for spike protein of multiple SARS-CoV-2 variants Le, Thao T. Benton, Donald J. Wrobel, Antoni G. Gamblin, Steven J. RSC Adv Chemistry We have developed broadly reactive aptamers against multiple variants by alternating the target between spike proteins from different SARS-CoV-2 variants during the selection process. In this process we have developed aptamers which can recognise all variants, from the original wild-type ‘Wuhan’ strain to Omicron, with high affinity (K(d) values in the pM range). The Royal Society of Chemistry 2023-05-19 /pmc/articles/PMC10197177/ /pubmed/37213341 http://dx.doi.org/10.1039/d3ra01382k Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by/3.0/
spellingShingle Chemistry
Le, Thao T.
Benton, Donald J.
Wrobel, Antoni G.
Gamblin, Steven J.
Development of high affinity broadly reactive aptamers for spike protein of multiple SARS-CoV-2 variants
title Development of high affinity broadly reactive aptamers for spike protein of multiple SARS-CoV-2 variants
title_full Development of high affinity broadly reactive aptamers for spike protein of multiple SARS-CoV-2 variants
title_fullStr Development of high affinity broadly reactive aptamers for spike protein of multiple SARS-CoV-2 variants
title_full_unstemmed Development of high affinity broadly reactive aptamers for spike protein of multiple SARS-CoV-2 variants
title_short Development of high affinity broadly reactive aptamers for spike protein of multiple SARS-CoV-2 variants
title_sort development of high affinity broadly reactive aptamers for spike protein of multiple sars-cov-2 variants
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10197177/
https://www.ncbi.nlm.nih.gov/pubmed/37213341
http://dx.doi.org/10.1039/d3ra01382k
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AT wrobelantonig developmentofhighaffinitybroadlyreactiveaptamersforspikeproteinofmultiplesarscov2variants
AT gamblinstevenj developmentofhighaffinitybroadlyreactiveaptamersforspikeproteinofmultiplesarscov2variants

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