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Upregulation of SPINK2 in acute myeloid leukemia
OBJECTIVES: Acute myeloid leukemia (AML) is a highly heterogeneous disease. Although patients can be classified into risk groups based on their genetic changes, the prognosis of disease within these categories varies widely. This situation raises the need to search for new molecular markers related...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
De Gruyter
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10197194/ https://www.ncbi.nlm.nih.gov/pubmed/37359898 http://dx.doi.org/10.1515/almed-2022-0047 |
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author | Gezer, Sümbül Emrence, Zeliha Elverdi, Tuğrul Ar, Muhlis Cem Salman Yaylaz, Burcu Paçal, Ferda Ünüvar, Ayşegül Sarıman, Melda Eşkazan, Ahmet Emre Karaman, Serap Salihoğlu, Ayşe Karakaş, Zeynep Abacı, Neslihan Sırma-Ekmekci, Sema |
author_facet | Gezer, Sümbül Emrence, Zeliha Elverdi, Tuğrul Ar, Muhlis Cem Salman Yaylaz, Burcu Paçal, Ferda Ünüvar, Ayşegül Sarıman, Melda Eşkazan, Ahmet Emre Karaman, Serap Salihoğlu, Ayşe Karakaş, Zeynep Abacı, Neslihan Sırma-Ekmekci, Sema |
author_sort | Gezer, Sümbül |
collection | PubMed |
description | OBJECTIVES: Acute myeloid leukemia (AML) is a highly heterogeneous disease. Although patients can be classified into risk groups based on their genetic changes, the prognosis of disease within these categories varies widely. This situation raises the need to search for new molecular markers related to AML. Serine peptidase inhibitor Kazal type 2 (SPINK2) has recently been reported to be upregulated in AML and associated with poor outcomes by meta-analysis and in a limited number of AML patients. METHODS: We analyzed SPINK2 mRNA expression in 62 patients (45 adult and 17 pediatric) with AML and 11 cell lines using quantitative Real-Time PCR (qRT-PCR). SPINK2 protein level was determined using ELISA in cell lines. RESULTS: We found that the expression of SPINK2 mRNA and protein levels in AML cell lines (HL60 and NB4) have increased compared to other cell lines (K562, Jurkat and NALM6, MCF7, HeLa, HUVEC, hFOB, 293T, U87). SPINK2 mRNA expression was upregulated in patients with AML compared to controls (p=0.004) and significantly lower in t(8;21)-positive patients compared to negative patients (p=0.0006). CONCLUSIONS: Our results suggest that SPINK2 serves an important role in AML development. Further studies are needed to evaluate SPINK2 expression in AML patients with t(8.21) and investigate to clarify its prognostic value in various subgroups of AML. |
format | Online Article Text |
id | pubmed-10197194 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | De Gruyter |
record_format | MEDLINE/PubMed |
spelling | pubmed-101971942023-06-23 Upregulation of SPINK2 in acute myeloid leukemia Gezer, Sümbül Emrence, Zeliha Elverdi, Tuğrul Ar, Muhlis Cem Salman Yaylaz, Burcu Paçal, Ferda Ünüvar, Ayşegül Sarıman, Melda Eşkazan, Ahmet Emre Karaman, Serap Salihoğlu, Ayşe Karakaş, Zeynep Abacı, Neslihan Sırma-Ekmekci, Sema Adv Lab Med Article OBJECTIVES: Acute myeloid leukemia (AML) is a highly heterogeneous disease. Although patients can be classified into risk groups based on their genetic changes, the prognosis of disease within these categories varies widely. This situation raises the need to search for new molecular markers related to AML. Serine peptidase inhibitor Kazal type 2 (SPINK2) has recently been reported to be upregulated in AML and associated with poor outcomes by meta-analysis and in a limited number of AML patients. METHODS: We analyzed SPINK2 mRNA expression in 62 patients (45 adult and 17 pediatric) with AML and 11 cell lines using quantitative Real-Time PCR (qRT-PCR). SPINK2 protein level was determined using ELISA in cell lines. RESULTS: We found that the expression of SPINK2 mRNA and protein levels in AML cell lines (HL60 and NB4) have increased compared to other cell lines (K562, Jurkat and NALM6, MCF7, HeLa, HUVEC, hFOB, 293T, U87). SPINK2 mRNA expression was upregulated in patients with AML compared to controls (p=0.004) and significantly lower in t(8;21)-positive patients compared to negative patients (p=0.0006). CONCLUSIONS: Our results suggest that SPINK2 serves an important role in AML development. Further studies are needed to evaluate SPINK2 expression in AML patients with t(8.21) and investigate to clarify its prognostic value in various subgroups of AML. De Gruyter 2023-02-20 /pmc/articles/PMC10197194/ /pubmed/37359898 http://dx.doi.org/10.1515/almed-2022-0047 Text en © 2023 the author(s), published by De Gruyter, Berlin/Boston https://creativecommons.org/licenses/by/4.0/This work is licensed under the Creative Commons Attribution 4.0 International License. |
spellingShingle | Article Gezer, Sümbül Emrence, Zeliha Elverdi, Tuğrul Ar, Muhlis Cem Salman Yaylaz, Burcu Paçal, Ferda Ünüvar, Ayşegül Sarıman, Melda Eşkazan, Ahmet Emre Karaman, Serap Salihoğlu, Ayşe Karakaş, Zeynep Abacı, Neslihan Sırma-Ekmekci, Sema Upregulation of SPINK2 in acute myeloid leukemia |
title | Upregulation of SPINK2 in acute myeloid leukemia |
title_full | Upregulation of SPINK2 in acute myeloid leukemia |
title_fullStr | Upregulation of SPINK2 in acute myeloid leukemia |
title_full_unstemmed | Upregulation of SPINK2 in acute myeloid leukemia |
title_short | Upregulation of SPINK2 in acute myeloid leukemia |
title_sort | upregulation of spink2 in acute myeloid leukemia |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10197194/ https://www.ncbi.nlm.nih.gov/pubmed/37359898 http://dx.doi.org/10.1515/almed-2022-0047 |
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