Cargando…
The Upstream Sequence Transcription Complex Dictates Nucleosome Positioning and Promoter Accessibility at piRNA Genes in the C. elegans Germ Line
The piRNA pathway is a conserved germline-specific small RNA pathway that ensures genomic integrity and continued fertility. In C. elegans and other nematodes, Type-I piRNA precursor transcripts are expressed from over 10,000 small, independently regulated genes clustered within two discrete domains...
Autores principales: | , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Cold Spring Harbor Laboratory
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10197682/ https://www.ncbi.nlm.nih.gov/pubmed/37215016 http://dx.doi.org/10.1101/2023.05.10.540274 |
_version_ | 1785044593291558912 |
---|---|
author | Sanchez, Nancy Gonzalez, Lauren E Reinke, Valerie |
author_facet | Sanchez, Nancy Gonzalez, Lauren E Reinke, Valerie |
author_sort | Sanchez, Nancy |
collection | PubMed |
description | The piRNA pathway is a conserved germline-specific small RNA pathway that ensures genomic integrity and continued fertility. In C. elegans and other nematodes, Type-I piRNA precursor transcripts are expressed from over 10,000 small, independently regulated genes clustered within two discrete domains of 1.5 and 3.5 MB on Chromosome IV. These large clusters likely play a significant role in promoting germline-specific expression of piRNAs, but the underlying mechanisms are unclear. By examining the chromatin environment specifically in isolated germ nuclei, we demonstrate that piRNA clusters are located in closed chromatin, and confirm the enrichment for the inactive histone modification H3K27me3. We further show that the piRNA biogenesis factor USTC (Upstream Sequence Transcription Complex) plays two roles – it promotes a strong association of nucleosomes throughout the piRNA clusters, and it organizes the local nucleosome environment to direct the exposure of individual piRNA genes. Overall, this work reveals new insight into how chromatin state coordinates transcriptional regulation over large genomic domains, which has implications for understanding global genome organization in the germ line. |
format | Online Article Text |
id | pubmed-10197682 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Cold Spring Harbor Laboratory |
record_format | MEDLINE/PubMed |
spelling | pubmed-101976822023-05-20 The Upstream Sequence Transcription Complex Dictates Nucleosome Positioning and Promoter Accessibility at piRNA Genes in the C. elegans Germ Line Sanchez, Nancy Gonzalez, Lauren E Reinke, Valerie bioRxiv Article The piRNA pathway is a conserved germline-specific small RNA pathway that ensures genomic integrity and continued fertility. In C. elegans and other nematodes, Type-I piRNA precursor transcripts are expressed from over 10,000 small, independently regulated genes clustered within two discrete domains of 1.5 and 3.5 MB on Chromosome IV. These large clusters likely play a significant role in promoting germline-specific expression of piRNAs, but the underlying mechanisms are unclear. By examining the chromatin environment specifically in isolated germ nuclei, we demonstrate that piRNA clusters are located in closed chromatin, and confirm the enrichment for the inactive histone modification H3K27me3. We further show that the piRNA biogenesis factor USTC (Upstream Sequence Transcription Complex) plays two roles – it promotes a strong association of nucleosomes throughout the piRNA clusters, and it organizes the local nucleosome environment to direct the exposure of individual piRNA genes. Overall, this work reveals new insight into how chromatin state coordinates transcriptional regulation over large genomic domains, which has implications for understanding global genome organization in the germ line. Cold Spring Harbor Laboratory 2023-05-12 /pmc/articles/PMC10197682/ /pubmed/37215016 http://dx.doi.org/10.1101/2023.05.10.540274 Text en https://creativecommons.org/licenses/by-nc-nd/4.0/This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which allows reusers to copy and distribute the material in any medium or format in unadapted form only, for noncommercial purposes only, and only so long as attribution is given to the creator. |
spellingShingle | Article Sanchez, Nancy Gonzalez, Lauren E Reinke, Valerie The Upstream Sequence Transcription Complex Dictates Nucleosome Positioning and Promoter Accessibility at piRNA Genes in the C. elegans Germ Line |
title | The Upstream Sequence Transcription Complex Dictates Nucleosome Positioning and Promoter Accessibility at piRNA Genes in the C. elegans Germ Line |
title_full | The Upstream Sequence Transcription Complex Dictates Nucleosome Positioning and Promoter Accessibility at piRNA Genes in the C. elegans Germ Line |
title_fullStr | The Upstream Sequence Transcription Complex Dictates Nucleosome Positioning and Promoter Accessibility at piRNA Genes in the C. elegans Germ Line |
title_full_unstemmed | The Upstream Sequence Transcription Complex Dictates Nucleosome Positioning and Promoter Accessibility at piRNA Genes in the C. elegans Germ Line |
title_short | The Upstream Sequence Transcription Complex Dictates Nucleosome Positioning and Promoter Accessibility at piRNA Genes in the C. elegans Germ Line |
title_sort | upstream sequence transcription complex dictates nucleosome positioning and promoter accessibility at pirna genes in the c. elegans germ line |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10197682/ https://www.ncbi.nlm.nih.gov/pubmed/37215016 http://dx.doi.org/10.1101/2023.05.10.540274 |
work_keys_str_mv | AT sancheznancy theupstreamsequencetranscriptioncomplexdictatesnucleosomepositioningandpromoteraccessibilityatpirnagenesinthecelegansgermline AT gonzalezlaurene theupstreamsequencetranscriptioncomplexdictatesnucleosomepositioningandpromoteraccessibilityatpirnagenesinthecelegansgermline AT reinkevalerie theupstreamsequencetranscriptioncomplexdictatesnucleosomepositioningandpromoteraccessibilityatpirnagenesinthecelegansgermline AT sancheznancy upstreamsequencetranscriptioncomplexdictatesnucleosomepositioningandpromoteraccessibilityatpirnagenesinthecelegansgermline AT gonzalezlaurene upstreamsequencetranscriptioncomplexdictatesnucleosomepositioningandpromoteraccessibilityatpirnagenesinthecelegansgermline AT reinkevalerie upstreamsequencetranscriptioncomplexdictatesnucleosomepositioningandpromoteraccessibilityatpirnagenesinthecelegansgermline |