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Light-field tomographic fluorescence lifetime imaging microscopy
Fluorescence lifetime imaging microscopy (FLIM) is a powerful imaging technique that enables the visualization of biological samples at the molecular level by measuring the fluorescence decay rate of fluorescent probes. This provides critical information about molecular interactions, environmental c...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Journal Experts
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10197779/ https://www.ncbi.nlm.nih.gov/pubmed/37214842 http://dx.doi.org/10.21203/rs.3.rs-2883279/v1 |
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author | Ma, Yayao Huang, Luzhe Sen, Chandani Burri, Samuel Bruschini, Claudio Yang, Xilin Cameron, Robert B. Fishbein, Gregory A. Gomperts, Brigitte N. Ozcan, Aydogan Charbon, Edoardo Gao, Liang |
author_facet | Ma, Yayao Huang, Luzhe Sen, Chandani Burri, Samuel Bruschini, Claudio Yang, Xilin Cameron, Robert B. Fishbein, Gregory A. Gomperts, Brigitte N. Ozcan, Aydogan Charbon, Edoardo Gao, Liang |
author_sort | Ma, Yayao |
collection | PubMed |
description | Fluorescence lifetime imaging microscopy (FLIM) is a powerful imaging technique that enables the visualization of biological samples at the molecular level by measuring the fluorescence decay rate of fluorescent probes. This provides critical information about molecular interactions, environmental changes, and localization within biological systems. However, creating high-resolution lifetime maps using conventional FLIM systems can be challenging, as it often requires extensive scanning that can significantly lengthen acquisition times. This issue is further compounded in three-dimensional (3D) imaging because it demands additional scanning along the depth axis. To tackle this challenge, we developed a novel computational imaging technique called light field tomographic FLIM (LIFT-FLIM). Our approach allows for the acquisition of volumetric fluorescence lifetime images in a highly data-efficient manner, significantly reducing the number of scanning steps required compared to conventional point-scanning or line-scanning FLIM imagers. Moreover, LIFT-FLIM enables the measurement of high-dimensional data using low-dimensional detectors, which are typically low-cost and feature a higher temporal bandwidth. We demonstrated LIFT-FLIM using a linear single-photon avalanche diode array on various biological systems, showcasing unparalleled single-photon detection sensitivity. Additionally, we expanded the functionality of our method to spectral FLIM and demonstrated its application in high-content multiplexed imaging of lung organoids. LIFT-FLIM has the potential to open up new avenues in both basic and translational biomedical research. |
format | Online Article Text |
id | pubmed-10197779 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | American Journal Experts |
record_format | MEDLINE/PubMed |
spelling | pubmed-101977792023-05-20 Light-field tomographic fluorescence lifetime imaging microscopy Ma, Yayao Huang, Luzhe Sen, Chandani Burri, Samuel Bruschini, Claudio Yang, Xilin Cameron, Robert B. Fishbein, Gregory A. Gomperts, Brigitte N. Ozcan, Aydogan Charbon, Edoardo Gao, Liang Res Sq Article Fluorescence lifetime imaging microscopy (FLIM) is a powerful imaging technique that enables the visualization of biological samples at the molecular level by measuring the fluorescence decay rate of fluorescent probes. This provides critical information about molecular interactions, environmental changes, and localization within biological systems. However, creating high-resolution lifetime maps using conventional FLIM systems can be challenging, as it often requires extensive scanning that can significantly lengthen acquisition times. This issue is further compounded in three-dimensional (3D) imaging because it demands additional scanning along the depth axis. To tackle this challenge, we developed a novel computational imaging technique called light field tomographic FLIM (LIFT-FLIM). Our approach allows for the acquisition of volumetric fluorescence lifetime images in a highly data-efficient manner, significantly reducing the number of scanning steps required compared to conventional point-scanning or line-scanning FLIM imagers. Moreover, LIFT-FLIM enables the measurement of high-dimensional data using low-dimensional detectors, which are typically low-cost and feature a higher temporal bandwidth. We demonstrated LIFT-FLIM using a linear single-photon avalanche diode array on various biological systems, showcasing unparalleled single-photon detection sensitivity. Additionally, we expanded the functionality of our method to spectral FLIM and demonstrated its application in high-content multiplexed imaging of lung organoids. LIFT-FLIM has the potential to open up new avenues in both basic and translational biomedical research. American Journal Experts 2023-05-10 /pmc/articles/PMC10197779/ /pubmed/37214842 http://dx.doi.org/10.21203/rs.3.rs-2883279/v1 Text en https://creativecommons.org/licenses/by/4.0/This work is licensed under a Creative Commons Attribution 4.0 International License (https://creativecommons.org/licenses/by/4.0/) , which allows reusers to distribute, remix, adapt, and build upon the material in any medium or format, so long as attribution is given to the creator. The license allows for commercial use. |
spellingShingle | Article Ma, Yayao Huang, Luzhe Sen, Chandani Burri, Samuel Bruschini, Claudio Yang, Xilin Cameron, Robert B. Fishbein, Gregory A. Gomperts, Brigitte N. Ozcan, Aydogan Charbon, Edoardo Gao, Liang Light-field tomographic fluorescence lifetime imaging microscopy |
title | Light-field tomographic fluorescence lifetime imaging microscopy |
title_full | Light-field tomographic fluorescence lifetime imaging microscopy |
title_fullStr | Light-field tomographic fluorescence lifetime imaging microscopy |
title_full_unstemmed | Light-field tomographic fluorescence lifetime imaging microscopy |
title_short | Light-field tomographic fluorescence lifetime imaging microscopy |
title_sort | light-field tomographic fluorescence lifetime imaging microscopy |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10197779/ https://www.ncbi.nlm.nih.gov/pubmed/37214842 http://dx.doi.org/10.21203/rs.3.rs-2883279/v1 |
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