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Cross-species activation of hydrogen cyanide production by a promiscuous quorum-sensing receptor promotes Chromobacterium subtsugae competition in a dual-species model

Many saprophytic bacteria have LuxR-I-type acyl-homoserine lactone (AHL) quorum-sensing systems that may be important for competing with other bacteria in complex soil communities. LuxR AHL receptors specifically interact with cognate AHLs to cause changes in expression of target genes. Some LuxR-ty...

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Autores principales: Loo, Cheyenne, Koirala, Pratik, Smith, Nathan C., Evans, Kara C., Benomar, Saida, Parisi, Isabelle R., Oller, Anna, Chandler, Josephine R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Microbiology Society 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10197875/
https://www.ncbi.nlm.nih.gov/pubmed/36790401
http://dx.doi.org/10.1099/mic.0.001294
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author Loo, Cheyenne
Koirala, Pratik
Smith, Nathan C.
Evans, Kara C.
Benomar, Saida
Parisi, Isabelle R.
Oller, Anna
Chandler, Josephine R.
author_facet Loo, Cheyenne
Koirala, Pratik
Smith, Nathan C.
Evans, Kara C.
Benomar, Saida
Parisi, Isabelle R.
Oller, Anna
Chandler, Josephine R.
author_sort Loo, Cheyenne
collection PubMed
description Many saprophytic bacteria have LuxR-I-type acyl-homoserine lactone (AHL) quorum-sensing systems that may be important for competing with other bacteria in complex soil communities. LuxR AHL receptors specifically interact with cognate AHLs to cause changes in expression of target genes. Some LuxR-type AHL receptors have relaxed specificity and are responsive to non-cognate AHLs. These promiscuous receptors might be used to sense and respond to AHLs produced by other bacteria by eavesdropping. We are interested in understanding the role of eavesdropping during interspecies competition. The soil saprophyte Chromobacterium subtsugae has a single AHL circuit, CviR-I, which produces and responds to N-hexanoyl-HSL (C6-HSL). The AHL receptor CviR can respond to a variety of AHLs in addition to C6-HSL. In prior studies we have utilized a coculture model with C. subtsugae and another soil saprophyte, Burkholderia thailandensis . Using this model, we previously showed that promiscuous activation of CviR by B. thailandensis AHLs provides a competitive advantage to C. subtsugae . Here, we show that B. thailandensis AHLs activate transcription of dozens of genes in C. subtsugae, including the hcnABC genes coding for production of hydrogen cyanide. We show that hydrogen cyanide production is population density-dependent and demonstrate that the cross-induction of hydrogen cyanide by B. thailandensis AHLs provides a competitive advantage to C. subtsugae . Our results provide new information on C. subtsugae quorum sensing and are the basis for future studies aimed at understanding the role of eavesdropping in interspecies competition.
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spelling pubmed-101978752023-05-20 Cross-species activation of hydrogen cyanide production by a promiscuous quorum-sensing receptor promotes Chromobacterium subtsugae competition in a dual-species model Loo, Cheyenne Koirala, Pratik Smith, Nathan C. Evans, Kara C. Benomar, Saida Parisi, Isabelle R. Oller, Anna Chandler, Josephine R. Microbiology (Reading) Regulation, Sensing and Signalling (formerly Regulation) Many saprophytic bacteria have LuxR-I-type acyl-homoserine lactone (AHL) quorum-sensing systems that may be important for competing with other bacteria in complex soil communities. LuxR AHL receptors specifically interact with cognate AHLs to cause changes in expression of target genes. Some LuxR-type AHL receptors have relaxed specificity and are responsive to non-cognate AHLs. These promiscuous receptors might be used to sense and respond to AHLs produced by other bacteria by eavesdropping. We are interested in understanding the role of eavesdropping during interspecies competition. The soil saprophyte Chromobacterium subtsugae has a single AHL circuit, CviR-I, which produces and responds to N-hexanoyl-HSL (C6-HSL). The AHL receptor CviR can respond to a variety of AHLs in addition to C6-HSL. In prior studies we have utilized a coculture model with C. subtsugae and another soil saprophyte, Burkholderia thailandensis . Using this model, we previously showed that promiscuous activation of CviR by B. thailandensis AHLs provides a competitive advantage to C. subtsugae . Here, we show that B. thailandensis AHLs activate transcription of dozens of genes in C. subtsugae, including the hcnABC genes coding for production of hydrogen cyanide. We show that hydrogen cyanide production is population density-dependent and demonstrate that the cross-induction of hydrogen cyanide by B. thailandensis AHLs provides a competitive advantage to C. subtsugae . Our results provide new information on C. subtsugae quorum sensing and are the basis for future studies aimed at understanding the role of eavesdropping in interspecies competition. Microbiology Society 2023-02-15 /pmc/articles/PMC10197875/ /pubmed/36790401 http://dx.doi.org/10.1099/mic.0.001294 Text en © 2023 The Authors https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License.
spellingShingle Regulation, Sensing and Signalling (formerly Regulation)
Loo, Cheyenne
Koirala, Pratik
Smith, Nathan C.
Evans, Kara C.
Benomar, Saida
Parisi, Isabelle R.
Oller, Anna
Chandler, Josephine R.
Cross-species activation of hydrogen cyanide production by a promiscuous quorum-sensing receptor promotes Chromobacterium subtsugae competition in a dual-species model
title Cross-species activation of hydrogen cyanide production by a promiscuous quorum-sensing receptor promotes Chromobacterium subtsugae competition in a dual-species model
title_full Cross-species activation of hydrogen cyanide production by a promiscuous quorum-sensing receptor promotes Chromobacterium subtsugae competition in a dual-species model
title_fullStr Cross-species activation of hydrogen cyanide production by a promiscuous quorum-sensing receptor promotes Chromobacterium subtsugae competition in a dual-species model
title_full_unstemmed Cross-species activation of hydrogen cyanide production by a promiscuous quorum-sensing receptor promotes Chromobacterium subtsugae competition in a dual-species model
title_short Cross-species activation of hydrogen cyanide production by a promiscuous quorum-sensing receptor promotes Chromobacterium subtsugae competition in a dual-species model
title_sort cross-species activation of hydrogen cyanide production by a promiscuous quorum-sensing receptor promotes chromobacterium subtsugae competition in a dual-species model
topic Regulation, Sensing and Signalling (formerly Regulation)
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10197875/
https://www.ncbi.nlm.nih.gov/pubmed/36790401
http://dx.doi.org/10.1099/mic.0.001294
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