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Role of Pel and Psl polysaccharides in the response of Pseudomonas aeruginosa to environmental challenges: oxidative stress agents (UVA, H(2)O(2), sodium hypochlorite) and its competitor Staphylococcus aureus

Pseudomonas aeruginosa is a versatile bacterium capable of adapting to a wide range of stress factors, including solar UVA radiation (400–315 nm). High UVA doses produce lethal effects due to the action of reactive oxygen species. Sublethal UVA doses also induces oxidative damage, but, in addition,...

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Autores principales: Grossich, Romina, Lemos Vilches, Martín, Costa, Cristina S., Pezzoni, Magdalena
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Microbiology Society 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10197878/
https://www.ncbi.nlm.nih.gov/pubmed/36757866
http://dx.doi.org/10.1099/mic.0.001301
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author Grossich, Romina
Lemos Vilches, Martín
Costa, Cristina S.
Pezzoni, Magdalena
author_facet Grossich, Romina
Lemos Vilches, Martín
Costa, Cristina S.
Pezzoni, Magdalena
author_sort Grossich, Romina
collection PubMed
description Pseudomonas aeruginosa is a versatile bacterium capable of adapting to a wide range of stress factors, including solar UVA radiation (400–315 nm). High UVA doses produce lethal effects due to the action of reactive oxygen species. Sublethal UVA doses also induces oxidative damage, but, in addition, it triggers a variety of adaptive responses, including the overexpression of pelA and pslA genes in P. aeruginosa . These genes encode the synthesis of Pel and Psl, which are essential polysaccharides in biofilm formation. The present study analysed the role of Pel and Psl in the adaptive responses generated by exposure to low UVA doses, and their importance in the response to lethal doses of UVA, hydrogen peroxide (H(2)O(2)), and sodium hypochlorite, in both planktonic cells and submerged and air–liquid interface (ALI) biofilms. It also studied the roles of Pel and Psl in P. aeruginosa–Staphylococcus aureus interaction. The results demonstrate that the capacity of sublethal UVA exposure to increase cell hydrophobicity and cell attachment and generate cross-protection phenomena in P. aeruginosa depends on the presence of Pel and Psl. The study also shows that Pel and Psl have a key role in the tolerance to lethal doses of UVA radiation, sodium hypochlorite and H(2)O(2), in both biofilms and planktonic cells. Finally, co-culture assays showed total inhibition of S. aureus growth in presence of P. aeruginosa . This phenomenon depends, at least in part, on the simultaneous presence of Pel and Psl in planktonic cells and biofilms, suggesting a relevant role of these polysaccharides in the interaction between these species.
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spelling pubmed-101978782023-05-20 Role of Pel and Psl polysaccharides in the response of Pseudomonas aeruginosa to environmental challenges: oxidative stress agents (UVA, H(2)O(2), sodium hypochlorite) and its competitor Staphylococcus aureus Grossich, Romina Lemos Vilches, Martín Costa, Cristina S. Pezzoni, Magdalena Microbiology (Reading) Microbial Physiology, Biochemistry and Metabolism Pseudomonas aeruginosa is a versatile bacterium capable of adapting to a wide range of stress factors, including solar UVA radiation (400–315 nm). High UVA doses produce lethal effects due to the action of reactive oxygen species. Sublethal UVA doses also induces oxidative damage, but, in addition, it triggers a variety of adaptive responses, including the overexpression of pelA and pslA genes in P. aeruginosa . These genes encode the synthesis of Pel and Psl, which are essential polysaccharides in biofilm formation. The present study analysed the role of Pel and Psl in the adaptive responses generated by exposure to low UVA doses, and their importance in the response to lethal doses of UVA, hydrogen peroxide (H(2)O(2)), and sodium hypochlorite, in both planktonic cells and submerged and air–liquid interface (ALI) biofilms. It also studied the roles of Pel and Psl in P. aeruginosa–Staphylococcus aureus interaction. The results demonstrate that the capacity of sublethal UVA exposure to increase cell hydrophobicity and cell attachment and generate cross-protection phenomena in P. aeruginosa depends on the presence of Pel and Psl. The study also shows that Pel and Psl have a key role in the tolerance to lethal doses of UVA radiation, sodium hypochlorite and H(2)O(2), in both biofilms and planktonic cells. Finally, co-culture assays showed total inhibition of S. aureus growth in presence of P. aeruginosa . This phenomenon depends, at least in part, on the simultaneous presence of Pel and Psl in planktonic cells and biofilms, suggesting a relevant role of these polysaccharides in the interaction between these species. Microbiology Society 2023-02-09 /pmc/articles/PMC10197878/ /pubmed/36757866 http://dx.doi.org/10.1099/mic.0.001301 Text en © 2023 The Authors https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License.
spellingShingle Microbial Physiology, Biochemistry and Metabolism
Grossich, Romina
Lemos Vilches, Martín
Costa, Cristina S.
Pezzoni, Magdalena
Role of Pel and Psl polysaccharides in the response of Pseudomonas aeruginosa to environmental challenges: oxidative stress agents (UVA, H(2)O(2), sodium hypochlorite) and its competitor Staphylococcus aureus
title Role of Pel and Psl polysaccharides in the response of Pseudomonas aeruginosa to environmental challenges: oxidative stress agents (UVA, H(2)O(2), sodium hypochlorite) and its competitor Staphylococcus aureus
title_full Role of Pel and Psl polysaccharides in the response of Pseudomonas aeruginosa to environmental challenges: oxidative stress agents (UVA, H(2)O(2), sodium hypochlorite) and its competitor Staphylococcus aureus
title_fullStr Role of Pel and Psl polysaccharides in the response of Pseudomonas aeruginosa to environmental challenges: oxidative stress agents (UVA, H(2)O(2), sodium hypochlorite) and its competitor Staphylococcus aureus
title_full_unstemmed Role of Pel and Psl polysaccharides in the response of Pseudomonas aeruginosa to environmental challenges: oxidative stress agents (UVA, H(2)O(2), sodium hypochlorite) and its competitor Staphylococcus aureus
title_short Role of Pel and Psl polysaccharides in the response of Pseudomonas aeruginosa to environmental challenges: oxidative stress agents (UVA, H(2)O(2), sodium hypochlorite) and its competitor Staphylococcus aureus
title_sort role of pel and psl polysaccharides in the response of pseudomonas aeruginosa to environmental challenges: oxidative stress agents (uva, h(2)o(2), sodium hypochlorite) and its competitor staphylococcus aureus
topic Microbial Physiology, Biochemistry and Metabolism
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10197878/
https://www.ncbi.nlm.nih.gov/pubmed/36757866
http://dx.doi.org/10.1099/mic.0.001301
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