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Molecular-electromechanical system for unamplified detection of trace analytes in biofluids

Biological research and diagnostic applications normally require analysis of trace analytes in biofluids. Although considerable advancements have been made in developing precise molecular assays, the trade-off between sensitivity and ability to resist non-specific adsorption remains a challenge. Her...

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Autores principales: Wang, Xuejun, Dai, Changhao, Wu, Yungeng, Liu, Yunqi, Wei, Dacheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10198040/
https://www.ncbi.nlm.nih.gov/pubmed/37208410
http://dx.doi.org/10.1038/s41596-023-00830-x
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author Wang, Xuejun
Dai, Changhao
Wu, Yungeng
Liu, Yunqi
Wei, Dacheng
author_facet Wang, Xuejun
Dai, Changhao
Wu, Yungeng
Liu, Yunqi
Wei, Dacheng
author_sort Wang, Xuejun
collection PubMed
description Biological research and diagnostic applications normally require analysis of trace analytes in biofluids. Although considerable advancements have been made in developing precise molecular assays, the trade-off between sensitivity and ability to resist non-specific adsorption remains a challenge. Here, we describe the implementation of a testing platform based on a molecular-electromechanical system (MolEMS) immobilized on graphene field-effect transistors. A MolEMS is a self-assembled DNA nanostructure, containing a stiff tetrahedral base and a flexible single-stranded DNA cantilever. Electromechanical actuation of the cantilever modulates sensing events close to the transistor channel, improving signal-transduction efficiency, while the stiff base prevents non-specific adsorption of background molecules present in biofluids. A MolEMS realizes unamplified detection of proteins, ions, small molecules and nucleic acids within minutes and has a limit of detection of several copies in 100 μl of testing solution, offering an assay methodology with wide-ranging applications. In this protocol, we provide step-by-step procedures for MolEMS design and assemblage, sensor manufacture and operation of a MolEMS in several applications. We also describe adaptations to construct a portable detection platform. It takes ~18 h to construct the device and ~4 min to finish the testing from sample addition to result.
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spelling pubmed-101980402023-05-23 Molecular-electromechanical system for unamplified detection of trace analytes in biofluids Wang, Xuejun Dai, Changhao Wu, Yungeng Liu, Yunqi Wei, Dacheng Nat Protoc Protocol Biological research and diagnostic applications normally require analysis of trace analytes in biofluids. Although considerable advancements have been made in developing precise molecular assays, the trade-off between sensitivity and ability to resist non-specific adsorption remains a challenge. Here, we describe the implementation of a testing platform based on a molecular-electromechanical system (MolEMS) immobilized on graphene field-effect transistors. A MolEMS is a self-assembled DNA nanostructure, containing a stiff tetrahedral base and a flexible single-stranded DNA cantilever. Electromechanical actuation of the cantilever modulates sensing events close to the transistor channel, improving signal-transduction efficiency, while the stiff base prevents non-specific adsorption of background molecules present in biofluids. A MolEMS realizes unamplified detection of proteins, ions, small molecules and nucleic acids within minutes and has a limit of detection of several copies in 100 μl of testing solution, offering an assay methodology with wide-ranging applications. In this protocol, we provide step-by-step procedures for MolEMS design and assemblage, sensor manufacture and operation of a MolEMS in several applications. We also describe adaptations to construct a portable detection platform. It takes ~18 h to construct the device and ~4 min to finish the testing from sample addition to result. Nature Publishing Group UK 2023-05-19 /pmc/articles/PMC10198040/ /pubmed/37208410 http://dx.doi.org/10.1038/s41596-023-00830-x Text en © Springer Nature Limited 2023, Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law. This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Protocol
Wang, Xuejun
Dai, Changhao
Wu, Yungeng
Liu, Yunqi
Wei, Dacheng
Molecular-electromechanical system for unamplified detection of trace analytes in biofluids
title Molecular-electromechanical system for unamplified detection of trace analytes in biofluids
title_full Molecular-electromechanical system for unamplified detection of trace analytes in biofluids
title_fullStr Molecular-electromechanical system for unamplified detection of trace analytes in biofluids
title_full_unstemmed Molecular-electromechanical system for unamplified detection of trace analytes in biofluids
title_short Molecular-electromechanical system for unamplified detection of trace analytes in biofluids
title_sort molecular-electromechanical system for unamplified detection of trace analytes in biofluids
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10198040/
https://www.ncbi.nlm.nih.gov/pubmed/37208410
http://dx.doi.org/10.1038/s41596-023-00830-x
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